Separation of Erythrocytes into Age-Related Fractions by Density or Size? Counterflow Centrifugation
-
Abstract
During the process of aging red blood cells become denser and smaller. Counterflow centrifugation separates particles of lower density and smaller diameter from those of higher density and bigger diameter. Thus, the question arises: which property of the red cells, density or size, governs the age-related separation by counterflow centrifugation? It is shown that it is the size which dominates the balance between sedimentation and streaming.
Age-related separation of human red blood cells by counterflow centrifugation (elutriation) was analysed by the standard hematological parameters (hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration), hemoglobin A1c and the membrane protein ratio 4.1a/(4.1a+4.1b). Red blood cells with a high hemoglobin A1c content and a high 4.1a/(4.1a+4.1b) ratio were found in the early fractions of the elutriation. This proves that old cells make up early fractions, while the “youngsters” constitute later ones.
The elutriation technique used (yielding human red blood cells in a “healthy state”) and the age parameters studied show that the membrane protein ratio 4.1a/(4.1a+4.1b) is another reliable age parameter for the assessment of red blood cell age.
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- SIBioC 2000 32nd National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology
- The Science of Systematic Reviewing Studies of Diagnostic Tests
- Need for Revisiting the Concept of Reference Values
- Purification of Anti-Thyroglobulin IgG from Human Serum
- Preeclampsia Is Associated with Alterations in Insulin-Like Growth Factor (IGF)-1 and IGF-Binding Proteins in Wharton's Jelly of the Umbilical Cord
- Sequential C3 and C4 Levels in Human Milk in Relation to Prematurity and Parity
- Evaluation of Emit® Tox Benzodiazepine and Barbiturate Assays on the Vitalab Viva Analyser and FPIA on the Abbott ADx Analyser
- Serum Eosinophil Cationic Protein in Active and Quiescent Ulcerative Colitis
- Measurement of Purines in Urine by Capillary Electrophoresis for Estimating the Degree of Hypoxia in Infants
- Separation of Erythrocytes into Age-Related Fractions by Density or Size? Counterflow Centrifugation
- Can Age and Sex Related Reference Intervals Be Derived for Non-Healthy and Non-Diseased Individuals from Results of Measurements in Primary Health Care?
- Associations of Apolipoprotein E Concentration and Polymorphism with Lipids and Apolipoprotein Levels in Chinese from Beijing and Shanghai
- Diagnostic Value of Uric Acid to Differentiate Transudates and Exudates
- Evaluation of the Roche Diagnostics LightCycler-Apo B 3500 Mutation Detection Kit
- HIV Infection Does Not Explain Elevation of Glycated Hemoglobin among Non-Diabetic Patients in Gabon
- Plasma and Serum total Homocysteine Concentrations in Paediatric Patients, Evaluated by High-Performance Liquid Chromatography with Fluorescence
- Lipoproteins in Health & Disease. By D. J. Betteridge, R. Illingworth and J. Shepherd
- Viral Hepatitis – Diagnosis, Therapy and Prevention. By Steven Specter, editor
Articles in the same Issue
- SIBioC 2000 32nd National Congress of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology
- The Science of Systematic Reviewing Studies of Diagnostic Tests
- Need for Revisiting the Concept of Reference Values
- Purification of Anti-Thyroglobulin IgG from Human Serum
- Preeclampsia Is Associated with Alterations in Insulin-Like Growth Factor (IGF)-1 and IGF-Binding Proteins in Wharton's Jelly of the Umbilical Cord
- Sequential C3 and C4 Levels in Human Milk in Relation to Prematurity and Parity
- Evaluation of Emit® Tox Benzodiazepine and Barbiturate Assays on the Vitalab Viva Analyser and FPIA on the Abbott ADx Analyser
- Serum Eosinophil Cationic Protein in Active and Quiescent Ulcerative Colitis
- Measurement of Purines in Urine by Capillary Electrophoresis for Estimating the Degree of Hypoxia in Infants
- Separation of Erythrocytes into Age-Related Fractions by Density or Size? Counterflow Centrifugation
- Can Age and Sex Related Reference Intervals Be Derived for Non-Healthy and Non-Diseased Individuals from Results of Measurements in Primary Health Care?
- Associations of Apolipoprotein E Concentration and Polymorphism with Lipids and Apolipoprotein Levels in Chinese from Beijing and Shanghai
- Diagnostic Value of Uric Acid to Differentiate Transudates and Exudates
- Evaluation of the Roche Diagnostics LightCycler-Apo B 3500 Mutation Detection Kit
- HIV Infection Does Not Explain Elevation of Glycated Hemoglobin among Non-Diabetic Patients in Gabon
- Plasma and Serum total Homocysteine Concentrations in Paediatric Patients, Evaluated by High-Performance Liquid Chromatography with Fluorescence
- Lipoproteins in Health & Disease. By D. J. Betteridge, R. Illingworth and J. Shepherd
- Viral Hepatitis – Diagnosis, Therapy and Prevention. By Steven Specter, editor
