Serum ras (p21) as a Marker for Occupationally Derived Lung Cancer?
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Joachim Schneider
Abstract
Certain subsets of the population are especially sensitive to carcinogens, and this can be determined using molecular biological methods. In the literature there has been evidence presented for the use of p21ras (ras) as a tumor marker for human carcinogenic substances such as asbestos, polycyclic aromatic hydrocarbons, and vinyl chloride in the workplace. In this study we have examined whether serum ras could serve as a biomarker for the early detection of occupationally derived lung cancer, with an emphasis on Schneeberger (radon-induced) lung cancer.
Sera were taken from 65 male tumor patients. Fifty-nine patients suffered from primary lung cancer (including 18 patients with Schneeberger lung cancer and 12 patients with asbestos-related lung cancer). Additionally, 29 patients with non-malignant lung disease, and a healthy control group (44) including 32 former uranium miners of SDAG Wismut exposed to ionizing radiation (radon and its decay products) were examined. Ras protein was determined via three different methods: 1) immunoprecipitation followed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting; 2) SDS-PAGE using 5–17% gradient gels followed by Western blotting; 3) pre-incubation with Blue Sepharose, SDS-PAGE on 5–17% gradient gels, and Western blotting.
The results show that 1 ng ras protein was measurable in serum standards. This protein could not be detected in patient sera or in sera from any of the study groups. Thus, ras cannot be considered useful as a marker for the early detection of asbestos-induced or Schneeberger lung
Copyright (c) 2000 by Walter de Gruyter GmbH & Co. KG
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- The Clinical Usefulness of the Measurement of Cytokines
- Measurement of Cholesterol and other Lipoprotein Constituents in the Clinical Laboratory
- Serum ras (p21) as a Marker for Occupationally Derived Lung Cancer?
- Macro Lactate Dehydrogenase in a Patient with Myocarditis
- Elimination of Serum Complexed Prostate-Specific Antigen after Radical Retropubic Prostatectomy
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- Comparison of the Abbott IMx® and a High-Performance Liquid Chromatography Method for Measuring Total Plasma Homocysteine
- Evaluation of the ADVIA® Centaur™ TSH-3 Assay
- Biochemical Composition and Electrolyte Balance of "Unstimulated" Whole Human Saliva
- Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination
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