Home Comparison of the Abbott IMx® and a High-Performance Liquid Chromatography Method for Measuring Total Plasma Homocysteine
Article
Licensed
Unlicensed Requires Authentication

Comparison of the Abbott IMx® and a High-Performance Liquid Chromatography Method for Measuring Total Plasma Homocysteine

  • Francisco Blanco-Vaca , Rosa Arcelús , Francesc González-Sastre , Jordi Ordóñez-Llanos and Josep M. Queraltó-Compañó
Published/Copyright: June 1, 2005
Become an author with De Gruyter Brill
Submit Manuscript Author Information
Clinical Chemistry and Laboratory Medicine (CCLM)
From the journal Volume 38 Issue 4

Abstract

The determination of the total concentration of plasma homocysteine is of interest in a variety of clinical circumstances, especially, in the evaluation of the risk of cardiovascular disease. However, most of the methods available to date, many of them chromatographic, are not well suited for the majority of clinical laboratories. Several automated methods are now or will be, shortly, commercially available. We have compared one of them, the fluorescence polarization immunoassay (FPIA) adapted to the IMx® analyzer (Abbott Laboratories), with the high-performance liquid chromatography (HPLC) method with fluorescent detection currently used in our laboratory. The results show that the FPIA-IMx® method is less imprecise and slightly more sensitive than the HPLC. The comparison of 67 clinical plasma specimens indicated that there is a proportional error disagreement between FPIA-IMx® and HPLC (FPIA=1.19 HPLC + 0.92; confidence region for slope and y-intercept were, respectively, from 1.06 to 1.31 and from−0.06 to 2.32). The nature of this error is not explained by the experiments performed to study the inaccuracy of both methods, which included the investigation of dilution parallelism, analytical recovery and cross-reactivity. The different results of homocysteine concentration obtained with FPIA-IMx® and HPLC must be taken into account when a change of methodology is under consideration.

:
Published Online: 2005-06-01
Published in Print: 2000-04-30

Copyright © 2000 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. The Clinical Usefulness of the Measurement of Cytokines
  2. Measurement of Cholesterol and other Lipoprotein Constituents in the Clinical Laboratory
  3. Serum ras (p21) as a Marker for Occupationally Derived Lung Cancer?
  4. Macro Lactate Dehydrogenase in a Patient with Myocarditis
  5. Elimination of Serum Complexed Prostate-Specific Antigen after Radical Retropubic Prostatectomy
  6. Suitability of Collection Tubes with Separator Gels for Collecting and Storing Blood Samples for Therapeutic Drug Monitoring (TDM)
  7. Influence of in vivo Hemoglobin Carbamylation on HbA1c Measurements by Various Methods
  8. Comparison of the Abbott IMx® and a High-Performance Liquid Chromatography Method for Measuring Total Plasma Homocysteine
  9. Evaluation of the ADVIA® Centaur™ TSH-3 Assay
  10. Biochemical Composition and Electrolyte Balance of "Unstimulated" Whole Human Saliva
  11. Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination
  12. Analytical and Clinical Evaluation of Troponin I Determination on Dimension RXL-HM
  13. Use of Ion-Selective Electrodes for Blood-Electrolyte Analysis. Recommendations for Nomenclature, Definitions and Conventions
  14. FESCC Survey on Accreditation and Post-Graduate Training in Clinical Chemistry in European Countries
  15. Clinical Use of Soluble Transferrin Receptor
  16. Macro Creatine Kinase Type 1: a Clinically Significant Marker?
  17. Vitamin B12 and B12-Proteins. By Bernhard Kräutler, Duilio Arigoni and Bernard T. Golding, editors
  18. The Bone and Mineral Manual – a Practical Guide. By Michael Kleerekoper, Ethel Sins, Michael McClung, editors
https://doi.org/10.1515/CCLM.2000.047

Articles in the same Issue

  1. The Clinical Usefulness of the Measurement of Cytokines
  2. Measurement of Cholesterol and other Lipoprotein Constituents in the Clinical Laboratory
  3. Serum ras (p21) as a Marker for Occupationally Derived Lung Cancer?
  4. Macro Lactate Dehydrogenase in a Patient with Myocarditis
  5. Elimination of Serum Complexed Prostate-Specific Antigen after Radical Retropubic Prostatectomy
  6. Suitability of Collection Tubes with Separator Gels for Collecting and Storing Blood Samples for Therapeutic Drug Monitoring (TDM)
  7. Influence of in vivo Hemoglobin Carbamylation on HbA1c Measurements by Various Methods
  8. Comparison of the Abbott IMx® and a High-Performance Liquid Chromatography Method for Measuring Total Plasma Homocysteine
  9. Evaluation of the ADVIA® Centaur™ TSH-3 Assay
  10. Biochemical Composition and Electrolyte Balance of "Unstimulated" Whole Human Saliva
  11. Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination
  12. Analytical and Clinical Evaluation of Troponin I Determination on Dimension RXL-HM
  13. Use of Ion-Selective Electrodes for Blood-Electrolyte Analysis. Recommendations for Nomenclature, Definitions and Conventions
  14. FESCC Survey on Accreditation and Post-Graduate Training in Clinical Chemistry in European Countries
  15. Clinical Use of Soluble Transferrin Receptor
  16. Macro Creatine Kinase Type 1: a Clinically Significant Marker?
  17. Vitamin B12 and B12-Proteins. By Bernhard Kräutler, Duilio Arigoni and Bernard T. Golding, editors
  18. The Bone and Mineral Manual – a Practical Guide. By Michael Kleerekoper, Ethel Sins, Michael McClung, editors
Sign up now to receive a 20% welcome discount
Institutional Access
How does access work?
Have an idea on how to improve our website?
Please write us.
© 2025 De Gruyter Brill
Downloaded on 11.10.2025 from https://www.degruyterbrill.com/document/doi/10.1515/CCLM.2000.047/html
Scroll to top button