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Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination
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Jürgen Westermann
Published/Copyright:
June 1, 2005
Abstract
A new commercially available enzyme-linked immunosorbent assay (ELISA) kit has been evaluated for the measurement of neopterin concentrations in serum, plasma and urine. This competitive ELISA is technically simple, requires only small sample volume and is rapid to perform. The assay procedure consists of sequential 1.5 h and 10 min room temperature incubation steps. The ELISA is accurate, sensitive, specific, and precise. Linear regression analysis of neopterin concentrations measured with the new ELISA and with an established method yielded a highly singificant correlation (r = 0.99). The new assay is applicable to ELISA workstations, thus enabling determination of neopterin in large series of samples. The neopterin ELISA kit has been used in routine laboratory testing of blood donations in a blood bank.
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Published Online: 2005-06-01
Published in Print: 2000-04-30
Copyright © 2000 by Walter de Gruyter GmbH & Co. KG
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Articles in the same Issue
- The Clinical Usefulness of the Measurement of Cytokines
- Measurement of Cholesterol and other Lipoprotein Constituents in the Clinical Laboratory
- Serum ras (p21) as a Marker for Occupationally Derived Lung Cancer?
- Macro Lactate Dehydrogenase in a Patient with Myocarditis
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- Suitability of Collection Tubes with Separator Gels for Collecting and Storing Blood Samples for Therapeutic Drug Monitoring (TDM)
- Influence of in vivo Hemoglobin Carbamylation on HbA1c Measurements by Various Methods
- Comparison of the Abbott IMx® and a High-Performance Liquid Chromatography Method for Measuring Total Plasma Homocysteine
- Evaluation of the ADVIA® Centaur™ TSH-3 Assay
- Biochemical Composition and Electrolyte Balance of "Unstimulated" Whole Human Saliva
- Evaluation of a New Simple and Rapid Enzyme-Linked Immunosorbent Assay Kit for Neopterin Determination
- Analytical and Clinical Evaluation of Troponin I Determination on Dimension RXL-HM
- Use of Ion-Selective Electrodes for Blood-Electrolyte Analysis. Recommendations for Nomenclature, Definitions and Conventions
- FESCC Survey on Accreditation and Post-Graduate Training in Clinical Chemistry in European Countries
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- Macro Creatine Kinase Type 1: a Clinically Significant Marker?
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