Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
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Kristina Stefansson
, Maria Brattsand , Annelii Ny , Bo Glas and Torbjörn Egelrud
Abstract
We have previously presented evidence that two human kallikrein-related peptidases, KLK5 (hK5, stratum corneum tryptic enzyme, SCTE) and KLK7 (hK7, stratum corneum chymotryptic enzyme, SCCE), which are abundant in the stratum corneum, may be involved in desquamation. Since we had noted that not all trypsin-like activity in the plantar stratum corneum could be ascribed to KLK5, we set out to identify other skin proteases with similar primary substrate specificity. Here we describe purification of a protease identified as KLK14 from plantar stratum corneum, and show that this enzyme may be responsible for as much as 50% of the total trypsin-like activity in this tissue, measured as activity towards a chromogenic substrate cleaved by a wide variety of enzymes with trypsin-like specificity. This was in spite of very low levels of KLK14 protein compared to KLK5 and KLK7. KLK14 could be detected by immunoblotting in normal superficial stratum corneum of all individuals examined. The majority of KLK14 in the plantar stratum corneum is present in its catalytically active form. KLK14 could be immunohistochemically detected in sweat ducts, preferentially in the intraepidermal parts (the acrosyringium), and in sweat glands. The role played by this very efficient protease under normal and disease conditions in the skin remains to be elucidated.
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
- The First International Symposium on Kallikreins
- A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
- The kallikrein world: an update on the human tissue kallikreins
- Cellular distribution of human tissue kallikreins: immunohistochemical localization
- The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
- Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
- Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
- Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
- The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
- Human kallikrein 10, a predictive marker for breast cancer
- Activation and enzymatic characterization of recombinant human kallikrein 8
- Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
- The human kallikrein 10 promoter contains a functional retinoid response element
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
- Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
- A sensitive proximity ligation assay for active PSA
- Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
- Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
- mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
- The epigenetic basis for the aberrant expression of kallikreins in human cancers
- Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
- Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
- Inhibition profiles of human tissue kallikreins by serine protease inhibitors
- Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)
Articles in the same Issue
- The First International Symposium on Kallikreins
- A comprehensive nomenclature for serine proteases with homology to tissue kallikreins
- The kallikrein world: an update on the human tissue kallikreins
- Cellular distribution of human tissue kallikreins: immunohistochemical localization
- The tissue kallikrein-kinin system protects against cardiovascular and renal diseases and ischemic stroke independently of blood pressure reduction
- Proteinase-mediated cell signalling: targeting proteinase-activated receptors (PARs) by kallikreins and more
- Recombinant kallikrein expression: site-specific integration for hK6 production in human cells
- Kallikrein-related peptidase (KLK) family mRNA variants and protein isoforms in hormone-related cancers: do they have a function?
- The role of kallikrein-related peptidases in prostate cancer: potential involvement in an epithelial to mesenchymal transition
- Human kallikrein 10, a predictive marker for breast cancer
- Activation and enzymatic characterization of recombinant human kallikrein 8
- Human tissue kallikrein 9: production of recombinant proteins and specific antibodies
- The human kallikrein 10 promoter contains a functional retinoid response element
- Human kallikrein 4: enzymatic activity, inhibition, and degradation of extracellular matrix proteins
- Kallikrein-related peptidase 14 may be a major contributor to trypsin-like proteolytic activity in human stratum corneum
- A sensitive proximity ligation assay for active PSA
- Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer
- Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues
- mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy
- The epigenetic basis for the aberrant expression of kallikreins in human cancers
- Improved prostate cancer detection with a human kallikrein 11 and percentage free PSA-based artificial neural network
- Overexpression of the human tissue kallikrein genes KLK4, 5, 6, and 7 increases the malignant phenotype of ovarian cancer cells
- Inhibition profiles of human tissue kallikreins by serine protease inhibitors
- Kallikrein-mediated cell signalling: targeting proteinase-activated receptors (PARs)