A fluorescence assay for rapid detection of ligand binding affinity to HIV-1 gp41
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Miriam Gochin
Abstract
The fusion-active conformation of the envelope protein gp41 of HIV-1 consists of an N-terminal trimeric α-helical coiled-coil domain and three anti-parallel C-terminal helices that fold down the grooves of the coiled-coil to form a six-helix bundle. Disruption of the six-helix bundle is considered to be a key component of an effective non-peptide fusion inhibitor. In the current study, a fluorescence resonance energy transfer (FRET) experiment for the detection of inhibitor binding to the gp41 N-peptide coiled-coil of HIV-1 was performed, utilizing peptide inhibitors derived from the gp41 C-terminal helical region. The FRET acceptor is a 31-residue N-peptide containing a known deep hydrophobic pocket, stabilized into a trimer by ferrous ion ligation. The FRET donor is a 16–18-residue fluorophore-labeled C-peptide, designed to test the specificity of the N-C interaction. Low μM dissociation constants were observed, correlated to the correct sequence and helical propensity of the C-peptides. Competitive inhibition was demonstrated using the assay, allowing for rank ordering of peptide inhibitors according to their affinity in the 1–20 μM range. The assay was conducted by measuring fluorescence intensity in 384-well plates. The rapid detection of inhibitor binding may permit identification of novel drug classes from a library.
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©2006 by Walter de Gruyter Berlin New York
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- Comparative proteomic analysis of neoplastic and non-neoplastic germ cell tissue
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- Identification of trypsin I as a candidate for influenza A virus and Sendai virus envelope glycoprotein processing protease in rat brain
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