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Evidence for attenuated cellular 8-oxo-7,8-dihydro-2′-deoxyguanosine removal in cancer patients

  • Marcus S. Cooke , Rafal Rozalski , Rosamund Dove , Daniel Gackowski , Agnieszka Siomek , Mark D. Evans and Ryszard Olinski
Published/Copyright: April 11, 2006
Biological Chemistry
From the journal Volume 387 Issue 4

Abstract

Measurement of the products of oxidatively damaged DNA in urine is a frequently used means by which oxidative stress may be assessed non-invasively. We believe that urinary DNA lesions, in addition to being biomarkers of oxidative stress, can potentially provide more specific information, for example, a reflection of repair activity. We used high-performance liquid chromatography prepurification, with gas chromatography-mass spectrometry (LC-GC-MS) and ELISA to the analysis of a number of oxidative [e.g., 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-guanine, 5-(hydroxymethyl)uracil], non-oxidative (cyclobutane thymine dimers) and oligomeric DNA products in urine. We analysed spot urine samples from 20 healthy subjects, and 20 age- and sex-matched cancer patients. Mononuclear cell DNA 8-oxodG levels were assessed by LC-EC. The data support our proposal that urinary DNA lesion products are predominantly derived from DNA repair. Furthermore, analysis of DNA and urinary 8-oxodG in cancer patients and controls suggested reduced repair activity towards this lesion marker in these patients.

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References

Ahmad, J., Cooke, M.S., Hussieni, A., Evans, M.D., Patel, K., Burd, R.M., et al. (1999). Urinary thymine dimers and 8-oxo-2′-deoxyguanosine in psoriasis. FEBS Lett.460, 549–553.10.1016/S0014-5793(99)01402-7Search in Google Scholar

Baron, D.N., Wicher, J.T., and Lee, K.E. (1989). A New Short Textbook of Chemical Pathology, 4th Edition (London, UK: Edward Arnold).Search in Google Scholar

Bessho, T., Tano, K., Kasai, H., Ohtsuka, E., and Nishimura, S. (1993). Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in human cells. J. Biol. Chem.268, 19416–19421.10.1016/S0021-9258(19)36531-7Search in Google Scholar

Bialkowski, K., Cysewski, P., and Olinski, R. (1996). Effect of 2′-deoxyguanosine oxidation at C8 position on N-glycosidic bond stability. Z. Naturforsch. C51, 119–122.10.1515/znc-1996-1-219Search in Google Scholar

Bianchini, F., Donato, F., Faure, H., Ravanat, J.L., Hall, J., and Cadet, J. (1998). Urinary excretion of 5-(hydroxymethyl) uracil in healthy volunteers: effect of active and passive tobacco smoke. Int. J. Cancer77, 40–46.10.1002/(SICI)1097-0215(19980703)77:1<40::AID-IJC8>3.0.CO;2-#Search in Google Scholar

Bogdanov, M.B., Beal, M.F., McCabe, D.R., Griffin, R.M., and Matson, W.R. (1999). A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2′-deoxyguanosine measurements in urine and other biologic matrices: a one-year evaluation of methods. Free Radic. Biol. Med.27, 647–666.10.1016/S0891-5849(99)00113-6Search in Google Scholar

Cathcart, R., Schwiers, E., Saul, R.L., and Ames, B.N. (1984). Thymine glycol and thymidine glycol in human and rat urine: a possible assay for oxidative DNA damage. Proc. Natl. Acad. Sci. USA81, 5633–5637.10.1073/pnas.81.18.5633Search in Google Scholar

Cleaver, J.E. (2005) Cancer in xeroderma pigmentosum and related disorders of DNA repair. Nat. Rev. Cancer5, 564–573.10.1038/nrc1652Search in Google Scholar

Cooke, M.S., Evans, M.D., Podmore, I.D., Herbert, K.E., Mistry, N., Mistry, P., et al. (1998). Novel repair action of vitamin C upon in vivo oxidative DNA damage. FEBS Lett.439, 363–367.10.1016/S0014-5793(98)01403-3Search in Google Scholar

Cooke, M.S., Evans, M.D., Herbert, K.E., and Lunec, J. (2000). Urinary 8-oxo-2′-deoxyguanosine – source, significance and supplements. Free Radic. Res.32, 381–397.10.1080/10715760000300391Search in Google Scholar

Cooke, M.S., Evans, M.D., Burd, R.M., Patel, K., Barnard, A., Lunec, J., et al. (2001a). Induction and excretion of ultraviolet-induced 8-oxo-2′-deoxyguanosine and thymine dimers in vivo: implications for PUVA. J. Invest. Dermatol.116, 281–285.10.1046/j.1523-1747.2001.01251.xSearch in Google Scholar

Cooke, M.S., Patel, K., Ahmad, J., Holloway, K., Evans, M.D., and Lunec, J. (2001b). Monoclonal antibody to single-stranded DNA: a potential tool for DNA repair studies. Biochem. Biophys. Res. Commun.284, 232–238.10.1006/bbrc.2001.4954Search in Google Scholar

Cooke, M.S., Evans, M.D., and Lunec, J. (2002a). DNA repair: insights from urinary lesion analysis. Free Radic. Res.36, 929–932.10.1080/1071576021000006635Search in Google Scholar

Cooke, M.S., Lunec, J., and Evans, M.D. (2002b). Progress in the analysis of urinary oxidative DNA damage. Free Radic. Biol. Med.33, 1601–1614.10.1016/S0891-5849(02)01146-2Search in Google Scholar

Cooke, M.S., Evans, M.D., Dizdaroglu, M., and Lunec, J. (2003a). Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J.17, 1195–1214.10.1096/fj.02-0752revSearch in Google Scholar

Cooke, M.S., Mistry, N., Ahmad, J., Waller, H., Langford, L., Bevan, R.J., et al. (2003b). Deoxycytidine glyoxal: lesion induction and evidence of repair following vitamin C supplementation in vivo. Free Radic. Biol. Med.34, 218–225.10.1016/S0891-5849(02)01240-6Search in Google Scholar

Cooke, M.S., Evans, M.D., Dove, R., Rozalski, R., Gackowski, D., Siomek, A., et al. (2005). DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine. Mutat. Res.574, 58–66.10.1016/j.mrfmmm.2005.01.022Search in Google Scholar

Dianov, G.L., Thybo, T., Dianova, I.I., Lipinski, L.J., and Bohr, V.A. (2000). Single nucleotide patch base excision repair is the major pathway for removal of thymine glycol from DNA in human cell extracts. J. Biol. Chem.275, 11809–11813.10.1074/jbc.275.16.11809Search in Google Scholar

Dizdaroglu, M. (1994). Chemical determination of oxidative DNA damage by gas chromatography-mass spectrometry. Methods Enzymol.234, 3–16.10.1016/0076-6879(94)34072-2Search in Google Scholar

Erhola, M., Toyokuni, S., Okada, K., Tanaka, T., Hiai, H., Ochi, H., et al. (1997). Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2′-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett.409, 287–291.10.1016/S0014-5793(97)00523-1Search in Google Scholar

Foksinski, M., Bialkowski, K., Skiba, M., Ponikowska, I., Szmurlo, W., and Olinski, R. (1999) Evaluation of 8-oxodeoxyguanosine, typical oxidative DNA damage, in lymphocytes of ozone-treated arteriosclerotic patients. Mutat. Res.438, 23–27.10.1016/S1383-5718(98)00155-7Search in Google Scholar

Foksinski, M., Rozalski, R., Guz, J., Ruszkowska, B., Sztukowska, P., Piwowarski, M., et al. (2004). Urinary excretion of DNA repair products correlates with metabolic rates as well as with maximum life spans of different mammalian species. Free Radic. Biol. Med.37, 1449–1454.10.1016/j.freeradbiomed.2004.07.014Search in Google Scholar PubMed

Fraga, C.G., Shigenaga, M.K., Park, J.W., Degan, P., and Ames, B.N. (1990). Oxidative damage to DNA during aging: 8-hydroxy-2′-deoxyguanosine in rat organ DNA and urine. Proc. Natl. Acad. Sci. USA87, 4533–4537.10.1073/pnas.87.12.4533Search in Google Scholar PubMed PubMed Central

Frank, S.A. (2004) Genetic predisposition to cancer – insights from population genetics. Nat. Rev. Genet.5, 764–772.10.1038/nrg1450Search in Google Scholar PubMed

Frosina, G. (2004) DNA base excision repair defects in human pathologies. Free Radic. Res.38, 1037–1054.10.1080/10715760400011445Search in Google Scholar PubMed

Gackowski, D., Rozalski, R., Roszkowski, K., Jawien, A., Foksinski, M., and Olinski, R. (2001). 8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in human urine do not depend on diet. Free Radic. Res.35, 825–832.10.1080/10715760100301321Search in Google Scholar PubMed

Gackowski, D., Kruszewski, M., Bartlomiejczyk, T., Jawien, A., Ciecierski, M., and Olinski, R. (2002). The level of 8-oxo-7,8-dihydro-2′-deoxyguanosine is positively correlated with the size of the labile iron pool in human lymphocytes. J. Biol. Inorg. Chem.7, 548–550.10.1007/s00775-001-0335-xSearch in Google Scholar PubMed

Gackowski, D., Speina, E., Zielinska, M., Kowalewski, J., Rozalski, R., Siomek, A., et al. (2003). Products of oxidative DNA damage and repair as possible biomarkers of susceptibility to lung cancer. Cancer Res.63, 4899–4902.Search in Google Scholar

Galloway, A.M., Liuzzi, M., and Paterson, M.C. (1994). Metabolic processing of cyclobutyl pyrimidine dimers and (6-4) photoproducts in UV-treated human cells. Evidence for distinct excision-repair pathways. J. Biol. Chem.269, 974–980.Search in Google Scholar

Helbock, H.J., Beckman, K.B., Shigenaga, M.K., Walter, P.B., Woodall, A.A., Yeo, H.C., et al. (1998). DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxodeoxyguanosine and 8-oxoguanine. Proc. Natl. Acad. Sci. USA95, 288–293.10.1073/pnas.95.1.288Search in Google Scholar PubMed PubMed Central

Lindahl, T. (1993). Instability and decay of the primary structure of DNA. Nature362, 709–715.10.1038/362709a0Search in Google Scholar PubMed

Loft, S. and Poulsen, H.E. (1998). Estimation of oxidative DNA damage in man from urinary excretion of repair products. Acta Biochim. Pol.45, 133–144.10.18388/abp.1998_4328Search in Google Scholar

Loft, S., Vistisen, K., Ewertz, M., Tjonneland, A., Overvad, K., and Poulsen, H.E. (1992). Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index. Carcinogenesis13, 2241–2247.10.1093/carcin/13.12.2241Search in Google Scholar PubMed

Lunec, J., Holloway, K.A., Cooke, M.S., Faux, S., Griffiths, H.R., and Evans, M.D. (2002). Urinary 8-oxo-2′-deoxyguanosine: redox regulation of DNA repair in vivo? Free Radic. Biol. Med.33, 875–885.Search in Google Scholar

Maki, H. and Sekiguchi, M. (1992). MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis. Nature355, 273–275.10.1038/355273a0Search in Google Scholar

Olinski, R., Foksinski, M., and Tudek, B. (2006). Oxidative DNA damage and carcinogenesis. In: Oxidative Damage to Nucleic Acids, M.D. Evans and M.S. Cooke, eds. (Georgetown, TX, USA: Landes Bioscience).Search in Google Scholar

Park, E.M., Shigenaga, M.K., Degan, P., Korn, T.S., Kitzler, J.W., Wehr, C.M., et al. (1992). Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc. Natl. Acad. Sci. USA89, 3375–3379.10.1073/pnas.89.8.3375Search in Google Scholar

Ravanat, J.L., Guicherd, P., Tuce, Z., and Cadet, J. (1999). Simultaneous determination of five oxidative DNA lesions in human urine. Chem. Res. Toxicol.12, 802–808.10.1021/tx980194kSearch in Google Scholar

Reardon, J.T., Bessho, T., Kung, H.C., Bolton, P.H., and Sancar, A. (1997). In vitro repair of oxidative DNA damage by human nucleotide excision repair system: possible explanation for neurodegeneration in xeroderma pigmentosum patients. Proc. Natl. Acad. Sci. USA94, 9463–9468.10.1073/pnas.94.17.9463Search in Google Scholar

Rosenquist, T.A., Zharkov, D.O., and Grollman, A.P. (1997). Cloning and characterization of a mammalian 8-oxoguanine DNA glycosylase. Proc. Natl. Acad. Sci. USA94, 7429–7434.10.1073/pnas.94.14.7429Search in Google Scholar

Rozalski, R., Gackowski, D., Roszkowski, K., Foksinski, M., and Olinski, R. (2002). The level of 8-hydroxyguanine, a possible repair product of oxidative DNA damage, is higher in urine of cancer patients than in control subjects. Cancer Epidemiol. Biomarkers Prev.11, 1072–1075.Search in Google Scholar

Rozalski, R., Siomek, A., Gackowski, D., Foksinski, M., Gran, C., Klungland, A., et al. (2004). Diet is not responsible for the presence of several oxidatively damaged DNA lesions in mouse urine. Free Radic. Res.38, 1201–1205.10.1080/10715760400017350Search in Google Scholar

Shigenaga, M.K., Gimeno, C.J., and Ames, B.N. (1989). Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of in vivo oxidative DNA damage. Proc. Natl. Acad. Sci. USA86, 9697–9701.10.1073/pnas.86.24.9697Search in Google Scholar

Shigenaga, M.K., Park, J.W., Cundy, K.C., Gimeno, C.J., and Ames, B.N. (1990). In vivo oxidative DNA damage: measurement of 8-hydroxy-2′-deoxyguanosine in DNA and urine by high-performance liquid chromatography with electrochemical detection. Methods Enzymol.186, 521–530.10.1016/0076-6879(90)86146-MSearch in Google Scholar

Shimoi, K., Kasai, H., Yokota, N., Toyokuni, S., and Kinae, N. (2002). Comparison between high-performance liquid chromatography and enzyme-linked immunosorbent assay for the determination of 8-hydroxy-2′-deoxyguanosine in human urine. Cancer Epidemiol. Biomarkers Prev.11, 767–770.Search in Google Scholar

Sokhansanj, B.A., Rodrigue, G.R., Fitch, J.P., and Wilson, D.M. III (2002). A quantitative model of human DNA base excision repair. I. Mechanistic insights. Nucleic Acids Res.30, 1817–1825.10.1093/nar/30.8.1817Search in Google Scholar PubMed PubMed Central

Toyokuni, S., Tanaka, T., Hattori, Y., Nishiyama, Y., Yoshida, A., Uchida, K., et al. (1997). Quantitative immunohistochemical determination of 8-hydroxy-2′-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Lab. Invest.76, 365–374.Search in Google Scholar

Tsuzuki, T., Egashira, A., Igarashi, H., Iwakuma, T., Nakatsuru, Y., Tominaga, Y., et al. (2001). Spontaneous tumorigenesis in mice defective in the MTH1 gene encoding 8-oxo-dGTPase. Proc. Natl. Acad. Sci. USA98, 11456–11461.10.1073/pnas.191086798Search in Google Scholar PubMed PubMed Central

Weimann, A., Belling, D., and Poulsen, H.E. (2002). Quantification of 8-oxo-guanine and guanine as the nucleobase, nucleoside and deoxynucleoside forms in human urine by high-performance liquid chromatography-electrospray tandem mass spectrometry. Nucleic Acids Res.30, E7.10.1093/nar/30.2.e7Search in Google Scholar PubMed PubMed Central

Published Online: 2006-04-11
Published in Print: 2006-04-01

©2006 by Walter de Gruyter Berlin New York

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