Evidence for attenuated cellular 8-oxo-7,8-dihydro-2′-deoxyguanosine removal in cancer patients
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Marcus S. Cooke
Abstract
Measurement of the products of oxidatively damaged DNA in urine is a frequently used means by which oxidative stress may be assessed non-invasively. We believe that urinary DNA lesions, in addition to being biomarkers of oxidative stress, can potentially provide more specific information, for example, a reflection of repair activity. We used high-performance liquid chromatography prepurification, with gas chromatography-mass spectrometry (LC-GC-MS) and ELISA to the analysis of a number of oxidative [e.g., 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydro-guanine, 5-(hydroxymethyl)uracil], non-oxidative (cyclobutane thymine dimers) and oligomeric DNA products in urine. We analysed spot urine samples from 20 healthy subjects, and 20 age- and sex-matched cancer patients. Mononuclear cell DNA 8-oxodG levels were assessed by LC-EC. The data support our proposal that urinary DNA lesion products are predominantly derived from DNA repair. Furthermore, analysis of DNA and urinary 8-oxodG in cancer patients and controls suggested reduced repair activity towards this lesion marker in these patients.
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
- Highlight: chronic oxidative stress and cancer
- Risk factors and mechanisms of hepatocarcinogenesis with special emphasis on alcohol and oxidative stress
- Does Helicobacter pylori cause gastric cancer via oxidative stress?
- Oxidative and nitrative DNA damage in animals and patients with inflammatory diseases in relation to inflammation-related carcinogenesis
- Mutagenesis and carcinogenesis caused by the oxidation of nucleic acids
- Concomitant suppression of hyperlipidemia and intestinal polyp formation by increasing lipoprotein lipase activity in Apc-deficient mice
- Cancer-preventive anti-oxidants that attenuate free radical generation by inflammatory cells
- Evidence for attenuated cellular 8-oxo-7,8-dihydro-2′-deoxyguanosine removal in cancer patients
- The roles of ATP in the dynamics of the actin filaments of the cytoskeleton
- Chiral distinction between the enantiomers of bicyclic alcohols by UDP-glucuronosyltransferases 2B7 and 2B17
- A structural model of 20S immunoproteasomes: effect of LMP2 codon 60 polymorphism on expression, activity, intracellular localisation and insight into the regulatory mechanisms
- Role of the kinin B1 receptor in insulin homeostasis and pancreatic islet function
- Comparative proteomic analysis of neoplastic and non-neoplastic germ cell tissue
- BID, an interaction partner of protein kinase CK2α
- Monomeric and dimeric GDF-5 show equal type I receptor binding and oligomerization capability and have the same biological activity
- Novel ketomethylene inhibitors of angiotensin I-converting enzyme (ACE): inhibition and molecular modelling
- Identification of trypsin I as a candidate for influenza A virus and Sendai virus envelope glycoprotein processing protease in rat brain
- A fluorescence assay for rapid detection of ligand binding affinity to HIV-1 gp41
Articles in the same Issue
- Highlight: chronic oxidative stress and cancer
- Risk factors and mechanisms of hepatocarcinogenesis with special emphasis on alcohol and oxidative stress
- Does Helicobacter pylori cause gastric cancer via oxidative stress?
- Oxidative and nitrative DNA damage in animals and patients with inflammatory diseases in relation to inflammation-related carcinogenesis
- Mutagenesis and carcinogenesis caused by the oxidation of nucleic acids
- Concomitant suppression of hyperlipidemia and intestinal polyp formation by increasing lipoprotein lipase activity in Apc-deficient mice
- Cancer-preventive anti-oxidants that attenuate free radical generation by inflammatory cells
- Evidence for attenuated cellular 8-oxo-7,8-dihydro-2′-deoxyguanosine removal in cancer patients
- The roles of ATP in the dynamics of the actin filaments of the cytoskeleton
- Chiral distinction between the enantiomers of bicyclic alcohols by UDP-glucuronosyltransferases 2B7 and 2B17
- A structural model of 20S immunoproteasomes: effect of LMP2 codon 60 polymorphism on expression, activity, intracellular localisation and insight into the regulatory mechanisms
- Role of the kinin B1 receptor in insulin homeostasis and pancreatic islet function
- Comparative proteomic analysis of neoplastic and non-neoplastic germ cell tissue
- BID, an interaction partner of protein kinase CK2α
- Monomeric and dimeric GDF-5 show equal type I receptor binding and oligomerization capability and have the same biological activity
- Novel ketomethylene inhibitors of angiotensin I-converting enzyme (ACE): inhibition and molecular modelling
- Identification of trypsin I as a candidate for influenza A virus and Sendai virus envelope glycoprotein processing protease in rat brain
- A fluorescence assay for rapid detection of ligand binding affinity to HIV-1 gp41