One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase
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Konstantin E. Komolov
Abstract
Recoverin is a neuronal calcium sensor protein that controls the activity of rhodopsin kinase in a Ca2+-dependent manner. Mutations in the EF-hand Ca2+ binding sites are valuable tools for investigating the functional properties of recoverin. In the recoverin mutant E121Q (RecE121Q) the high-affinity Ca2+ binding site is disabled. The non-myristoylated form of RecE121Q binds one Ca2+ via its second Ca2+-binding site (EF-hand 2), whereas the myristoylated variant does not bind Ca2+ at all. Binding of Ca2+ to non-myristoylated RecE121Q apparently triggers exposure of apolar side chains, allowing for association with hydrophobic matrices. Likewise, an interaction surface for the recoverin target rhodopsin kinase is constituted upon Ca2+ binding to the non-acylated mutant. Structural changes resulting from Ca2+-occupation of EF-hand 2 in myristoylated and non-myristoylated recoverin variants are discussed in terms of critical conditions required for biological activity.
References
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Artikel in diesem Heft
- Signaling in Biochemical Pharmacology and Toxicology
- Oncogenic Ras in tumour progression and metastasis
- Phosphoinositide 3-kinase signaling in the cellular response to oxidative stress
- Doxorubicin induces EGF receptor-dependent downregulation of gap junctional intercellular communication in rat liver epithelial cells
- TGFβ-induced focal complex formation in epithelial cells is mediated by activated ERK and JNK MAP kinases and is independent of Smad4
- On the mechanism of alkylphosphocholine (APC)-induced apoptosis in tumour cells
- Self-organization versus Watchmaker: stochastic dynamics of cellular organization
- Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex
- Trehalose and 6-aminohexanoic acid stabilize and renature glucose-6-phosphate dehydrogenase inactivated by glycation and by guanidinium hydrochloride
- Quercetin metabolism in vital and apoptotic human leukaemia cells
- One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase
- Enzymatic profiling of human kallikrein 14 using phage-display substrate technology
- A new selective substrate for cathepsin E based on the cleavage site sequence of α2-macroglobulin