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Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex

  • Emmanuel Di Valentin , Sébastien Bontems , Lionel Habran , Olivier Jolois , Nicolas Markine-Goriaynoff , Alain Vanderplasschen , Catherine Sadzot-Delvaux and Jacques Piette
Published/Copyright: July 5, 2005
Biological Chemistry
From the journal Volume 386 Issue 3

Abstract

Using transient transfection assays, regulation properties of varicella-zoster virus (VZV)-encoded IE63 protein were analyzed on several VZV immediate early (ORF4), early (ORF28) and late (ORF67) promoters. IE63 was shown to repress the basal activity of most of the promoters tested in epithelial (Vero) and neuronal (ND7) cells to various extents. Trans-repressing activities were also observed on heterologous viral and cellular promoters. Since a construct carrying only a TATA box sequence and a series of wild-type or mutated interleukin (IL)-8 promoters was also repressed by IE63, the role of upstream regulatory elements was ruled out. Importantly, the basal activity of a TATA-less promoter was not affected by IE63. Using a series of IE63 deletion constructs, amino acids 151–213 were shown to be essential to the trans-repressing activity in Vero cells, while in ND7 cells the essential region extended to a much larger carboxy-terminal part of the protein. We also demonstrate that IE63 is capable of disrupting the transcriptional pre-initiation complex and of interacting with several general transcription factors. The central and carboxy-terminal domains of IE63 are important for these effects. Altogether, these results demonstrate that IE63 protein is a transcriptional repressor whose activity is directed towards general transcription factors.

Keywords: down-regulation; general transcription factors; repression; transcription; varicella-zoster
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Published Online: 2005-07-05
Published in Print: 2005-03-01

©2004 by Walter de Gruyter Berlin New York

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https://doi.org/10.1515/BC.2005.031
Keywords for this article
down-regulation; general transcription factors; repression; transcription; varicella-zoster

Articles in the same Issue

  1. Signaling in Biochemical Pharmacology and Toxicology
  2. Oncogenic Ras in tumour progression and metastasis
  3. Phosphoinositide 3-kinase signaling in the cellular response to oxidative stress
  4. Doxorubicin induces EGF receptor-dependent downregulation of gap junctional intercellular communication in rat liver epithelial cells
  5. TGFβ-induced focal complex formation in epithelial cells is mediated by activated ERK and JNK MAP kinases and is independent of Smad4
  6. On the mechanism of alkylphosphocholine (APC)-induced apoptosis in tumour cells
  7. Self-organization versus Watchmaker: stochastic dynamics of cellular organization
  8. Varicella-zoster virus IE63 protein represses the basal transcription machinery by disorganizing the pre-initiation complex
  9. Trehalose and 6-aminohexanoic acid stabilize and renature glucose-6-phosphate dehydrogenase inactivated by glycation and by guanidinium hydrochloride
  10. Quercetin metabolism in vital and apoptotic human leukaemia cells
  11. One of the Ca2+ binding sites of recoverin exclusively controls interaction with rhodopsin kinase
  12. Enzymatic profiling of human kallikrein 14 using phage-display substrate technology
  13. A new selective substrate for cathepsin E based on the cleavage site sequence of α2-macroglobulin
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