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Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis

  • Masahiko Shiraishi , Adam J. Oates , Xu Li , Ying H. Chuu and Takao Sekiya
Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 385 Issue 10

Abstract

The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed.

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Published Online: 2005-06-01
Published in Print: 2004-10-01

© Walter de Gruyter

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