Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis
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Masahiko Shiraishi
, Adam J. Oates , Xu Li , Ying H. Chuu and Takao Sekiya
Abstract
The technique of segregation of partly melted molecules (SPM) is a convenient and efficient method to isolate DNA fragments associated with CpG islands. The approach is conceptually simple and uses denaturant gradient gel electrophoresis to separate DNA molecules digested with restriction endonucleases. The SPM methodology has successfully been applied to the identification of genes from anonymous, unsequenced DNA fragments and CpG islands methylated in human cancer. In this article the theoretical background and practical application of the SPM method is reviewed.
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Articles in the same Issue
- Wolfgang Baumeister – Felix Hoppe-Seyler Lecturer 2004
- Mapping molecular landscapes inside cells
- Paper of the Year 2003: Award to Dieter Hoffmann
- Structural basis of denitrification
- Roles of nectins in cell adhesion, migration and polarization
- Designing novel spectral classes of proteins with a tryptophan-expanded genetic code
- Imprinted small RNA genes
- Inter- and intra-molecular distances determined by EPR spectroscopy and site-directed spin labeling reveal protein-protein and protein-oligonucleotide interaction
- Combined transport of water and ions through membrane channels
- How do ABC transporters drive transport?
- X-ray structure of fumarylacetoacetate hydrolase family member Homo sapiens FLJ36880
- Generation of glycogen- and albumin-producing hepatocyte-like cells from embryonic stem cells
- Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM
- A database search for double-strand containing RNAs in Dictyostelium discoideum
- Segregation of partly melted molecules: isolation of CpG islands by polyacrylamide gel electrophoresis
- A monomeric mutant of restriction endonuclease EcoRI nicks DNA without sequence specificity