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Evidence for the Involvement of the Unique C-Tail of S100A9 in the Binding of Arachidonic Acid to the Heterocomplex S100A8/A9

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 383 Issue 12

Abstract

Protein complexes formed by S100A8 and S100A9 represent the only AAbinding capacity in the human neutrophilic cytosol and are involved in the intracellular arachidonic acid metabolism. The formation of S100A8/A9 protein complexes and the binding of calcium to the complexes are prerequisites for the specific binding of polyunsaturated fatty acids. The present study was undertaken to characterize the fatty acid binding site within the protein complex. Deletions at both termini and point mutations of different basic amino acids especially within the extended Cterminal tail of human S100A9 were introduced. The S100A9 mutant proteins were then analyzed with respect to proteinprotein interaction (GST pull downassay and yeast twohybrid system) and functional properties (arachidonic acid and calcium binding). The data give strong evidence that the unique Ctail of S100A9 containing the three consecutive histidine residues (His103-His105) represents the region to which the fatty acid carboxygroup is bound to the protein complex. The localization of the AAbinding site within the unique Ctail of S100A9 correlates with the fact that fatty acid binding has not yet been reported for other S100 proteins.

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Published Online: 2005-06-01
Published in Print: 2002-12-09

Copyright © 2002 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Processing and Activation of Lysosomal Proteinases
  2. Evidence That E-Box Promoter Elements and MyoD Transcription Factors Play a Role in the Induction of Cathepsin B Gene Expression during Human Myoblast Differentiation
  3. Transcription Factor Egr-1 Activates Collagen Expression in Immortalized Fibroblasts or Fibrosarcoma Cells
  4. The Receptor-Bound N-Terminal Ectodomain of the Amyloid Precursor Protein Is Associated with Membrane Rafts
  5. Evaluation of Similarities in the cis/trans Isomerase Function of Trigger Factor and DnaK
  6. The Chloroplast Import Receptor Toc34 Functions as Preprotein-Regulated GTPase
  7. cis-4-Methylsphingosine Phosphate Induces Apoptosis in Neuroblastoma Cells by Opposite Effects on p38 and ERK Mitogen-Activated Protein Kinases
  8. Evidence for the Involvement of the Unique C-Tail of S100A9 in the Binding of Arachidonic Acid to the Heterocomplex S100A8/A9
  9. The Primary Structure of Three Hemoglobin Chains from the Indigo Snake (Drymarchon corais erebennus, Serpentes): First Evidence for αD Chains and Two β Chain Types in Snakes
  10. Expression of the Tissue Kallikrein-Kinin Cascade in Granulosa Cells of the Ovary
  11. Chondroitin Sulfate Proteoglycan Is a Potent Enhancer in the Processing of Procathepsin L
  12. Tyrosinase-Mediated Oxidation of Acetaminophen to 4-Acetamido-o- Benzoquinone
  13. Properties of Baculovirus Particles Displaying GFP Analyzed by Fluorescence Correlation Spectroscopy
  14. The Promoter Context Determines Mutual Repression or Synergism between NF-κB and the Glucocorticoid Receptor
  15. Expression of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2) in Human Testis: Identification of a Distinct Transcription Start Site for the HAI-2 Gene in Testis
  16. Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland
  17. Characterization of Different Reactive Lysines in Bovine Heart Mitochondrial Porin
  18. Determination of the mRNA Sequence of Cathepsin Y, a Cysteine Endopeptidase from Rat Spleen, and Confirmation of Its Ubiquitous
  19. Acknowledgement
  20. Content Index
  21. Author Index
  22. Subject Index
https://doi.org/10.1515/BC.2002.213

Articles in the same Issue

  1. Processing and Activation of Lysosomal Proteinases
  2. Evidence That E-Box Promoter Elements and MyoD Transcription Factors Play a Role in the Induction of Cathepsin B Gene Expression during Human Myoblast Differentiation
  3. Transcription Factor Egr-1 Activates Collagen Expression in Immortalized Fibroblasts or Fibrosarcoma Cells
  4. The Receptor-Bound N-Terminal Ectodomain of the Amyloid Precursor Protein Is Associated with Membrane Rafts
  5. Evaluation of Similarities in the cis/trans Isomerase Function of Trigger Factor and DnaK
  6. The Chloroplast Import Receptor Toc34 Functions as Preprotein-Regulated GTPase
  7. cis-4-Methylsphingosine Phosphate Induces Apoptosis in Neuroblastoma Cells by Opposite Effects on p38 and ERK Mitogen-Activated Protein Kinases
  8. Evidence for the Involvement of the Unique C-Tail of S100A9 in the Binding of Arachidonic Acid to the Heterocomplex S100A8/A9
  9. The Primary Structure of Three Hemoglobin Chains from the Indigo Snake (Drymarchon corais erebennus, Serpentes): First Evidence for αD Chains and Two β Chain Types in Snakes
  10. Expression of the Tissue Kallikrein-Kinin Cascade in Granulosa Cells of the Ovary
  11. Chondroitin Sulfate Proteoglycan Is a Potent Enhancer in the Processing of Procathepsin L
  12. Tyrosinase-Mediated Oxidation of Acetaminophen to 4-Acetamido-o- Benzoquinone
  13. Properties of Baculovirus Particles Displaying GFP Analyzed by Fluorescence Correlation Spectroscopy
  14. The Promoter Context Determines Mutual Repression or Synergism between NF-κB and the Glucocorticoid Receptor
  15. Expression of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2) in Human Testis: Identification of a Distinct Transcription Start Site for the HAI-2 Gene in Testis
  16. Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland
  17. Characterization of Different Reactive Lysines in Bovine Heart Mitochondrial Porin
  18. Determination of the mRNA Sequence of Cathepsin Y, a Cysteine Endopeptidase from Rat Spleen, and Confirmation of Its Ubiquitous
  19. Acknowledgement
  20. Content Index
  21. Author Index
  22. Subject Index
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