Evaluation of Similarities in the cis/trans Isomerase Function of Trigger Factor and DnaK
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C. Schiene-Fischer
Abstract
* Corresponding author Two functionally redundant enzymes, trigger and the hsp70 chaperone DnaK, have been assist de novo protein folding in E. coli. Trigger is a peripheral peptidyl prolyl cis/trans isomerase (PPIase) of the large subunit of the ribosome. contrast, DnaK displays two catalytic features: secondary amide peptide bond cis/trans isomerase (APIase) function supplemented by the ATPase APIases accelerate the cis/trans isomerization nonprolyl peptide bonds. Both enzymes have for an unfolded polypeptide chain. The diminished temperature cell viability in the presence factor variants with impaired PPIase activity that the enhancement of folding rates plays role in protein folding in vivo. For the DnaK increase in the folding yield in vitro, the minimal model for APIase catalysis involves the catalyzed partitioning of a rapidly formed folding intermediate could be inferred from the DnaK/DnaJ/GrpE/ assisted refolding of GdmCldenatured luciferase. Using three different peptide bond cis/trans isomerization assays in vitro, we could show that no overlapping substrate specificity of trigger and DnaK. We propose that only if trigger recruits supplementing molecules is it capable exhibiting functional complementarity with DnaK protein folding.
Copyright © 2002 by Walter de Gruyter GmbH & Co. KG
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- Properties of Baculovirus Particles Displaying GFP Analyzed by Fluorescence Correlation Spectroscopy
- The Promoter Context Determines Mutual Repression or Synergism between NF-κB and the Glucocorticoid Receptor
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- Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland
- Characterization of Different Reactive Lysines in Bovine Heart Mitochondrial Porin
- Determination of the mRNA Sequence of Cathepsin Y, a Cysteine Endopeptidase from Rat Spleen, and Confirmation of Its Ubiquitous
- Acknowledgement
- Content Index
- Author Index
- Subject Index
Articles in the same Issue
- Processing and Activation of Lysosomal Proteinases
- Evidence That E-Box Promoter Elements and MyoD Transcription Factors Play a Role in the Induction of Cathepsin B Gene Expression during Human Myoblast Differentiation
- Transcription Factor Egr-1 Activates Collagen Expression in Immortalized Fibroblasts or Fibrosarcoma Cells
- The Receptor-Bound N-Terminal Ectodomain of the Amyloid Precursor Protein Is Associated with Membrane Rafts
- Evaluation of Similarities in the cis/trans Isomerase Function of Trigger Factor and DnaK
- The Chloroplast Import Receptor Toc34 Functions as Preprotein-Regulated GTPase
- cis-4-Methylsphingosine Phosphate Induces Apoptosis in Neuroblastoma Cells by Opposite Effects on p38 and ERK Mitogen-Activated Protein Kinases
- Evidence for the Involvement of the Unique C-Tail of S100A9 in the Binding of Arachidonic Acid to the Heterocomplex S100A8/A9
- The Primary Structure of Three Hemoglobin Chains from the Indigo Snake (Drymarchon corais erebennus, Serpentes): First Evidence for αD Chains and Two β Chain Types in Snakes
- Expression of the Tissue Kallikrein-Kinin Cascade in Granulosa Cells of the Ovary
- Chondroitin Sulfate Proteoglycan Is a Potent Enhancer in the Processing of Procathepsin L
- Tyrosinase-Mediated Oxidation of Acetaminophen to 4-Acetamido-o- Benzoquinone
- Properties of Baculovirus Particles Displaying GFP Analyzed by Fluorescence Correlation Spectroscopy
- The Promoter Context Determines Mutual Repression or Synergism between NF-κB and the Glucocorticoid Receptor
- Expression of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2) in Human Testis: Identification of a Distinct Transcription Start Site for the HAI-2 Gene in Testis
- Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland
- Characterization of Different Reactive Lysines in Bovine Heart Mitochondrial Porin
- Determination of the mRNA Sequence of Cathepsin Y, a Cysteine Endopeptidase from Rat Spleen, and Confirmation of Its Ubiquitous
- Acknowledgement
- Content Index
- Author Index
- Subject Index
