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Transcription Factor Egr-1 Activates Collagen Expression in Immortalized Fibroblasts or Fibrosarcoma Cells

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Published/Copyright: June 1, 2005
Biological Chemistry
From the journal Volume 383 Issue 12

Abstract

Synovial fibroblasts from rheumatoid arthritis patients express elevated levels of the transcription factor Egr 1. The metabolic consequences of Egr-1 overexpression in fibroblasts are not known in detail. Therefore we searched for gene products that are differentially expressed in Egr-1high versus Egr-1low fibroblasts. Immortalized synovial fibroblasts were transfected with two different Egr-1 expression vectors. Expression of recombinant Egr-1 was confirmed by RTPCR and immunoblots. Random arbitrarily primed PCR revealed that Egr-1 induces enhanced transcription levels of the α1 chain of type I collagen. Increased expression of the α2 (I) chain could also be observed. We found enhanced levels of type I collagen propeptide in supernatants and stronger signals of α2 (I) protein in extracts of the Egr 1high expressing clone versus controls. Additionally, Egr-1 was transiently expressed in fibrosarcoma cells. These cells showed a pronounced elevation of type I collagen (α1) transcripts as well. Moreover, we could demonstrate that Egr-1 induces transcription of other genes including type II collagen (α1) and plateledderived growth factor β1. These data suggest that upregulation of Egr-1 might contribute to fibrosis observed in rheumatoid arthritis synovium by activation of genes encoding the α1 and α2 chains of type I collagen.

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Published Online: 2005-06-01
Published in Print: 2002-12-09

Copyright © 2002 by Walter de Gruyter GmbH & Co. KG

Articles in the same Issue

  1. Processing and Activation of Lysosomal Proteinases
  2. Evidence That E-Box Promoter Elements and MyoD Transcription Factors Play a Role in the Induction of Cathepsin B Gene Expression during Human Myoblast Differentiation
  3. Transcription Factor Egr-1 Activates Collagen Expression in Immortalized Fibroblasts or Fibrosarcoma Cells
  4. The Receptor-Bound N-Terminal Ectodomain of the Amyloid Precursor Protein Is Associated with Membrane Rafts
  5. Evaluation of Similarities in the cis/trans Isomerase Function of Trigger Factor and DnaK
  6. The Chloroplast Import Receptor Toc34 Functions as Preprotein-Regulated GTPase
  7. cis-4-Methylsphingosine Phosphate Induces Apoptosis in Neuroblastoma Cells by Opposite Effects on p38 and ERK Mitogen-Activated Protein Kinases
  8. Evidence for the Involvement of the Unique C-Tail of S100A9 in the Binding of Arachidonic Acid to the Heterocomplex S100A8/A9
  9. The Primary Structure of Three Hemoglobin Chains from the Indigo Snake (Drymarchon corais erebennus, Serpentes): First Evidence for αD Chains and Two β Chain Types in Snakes
  10. Expression of the Tissue Kallikrein-Kinin Cascade in Granulosa Cells of the Ovary
  11. Chondroitin Sulfate Proteoglycan Is a Potent Enhancer in the Processing of Procathepsin L
  12. Tyrosinase-Mediated Oxidation of Acetaminophen to 4-Acetamido-o- Benzoquinone
  13. Properties of Baculovirus Particles Displaying GFP Analyzed by Fluorescence Correlation Spectroscopy
  14. The Promoter Context Determines Mutual Repression or Synergism between NF-κB and the Glucocorticoid Receptor
  15. Expression of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2) in Human Testis: Identification of a Distinct Transcription Start Site for the HAI-2 Gene in Testis
  16. Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland
  17. Characterization of Different Reactive Lysines in Bovine Heart Mitochondrial Porin
  18. Determination of the mRNA Sequence of Cathepsin Y, a Cysteine Endopeptidase from Rat Spleen, and Confirmation of Its Ubiquitous
  19. Acknowledgement
  20. Content Index
  21. Author Index
  22. Subject Index
https://doi.org/10.1515/BC.2002.208

Articles in the same Issue

  1. Processing and Activation of Lysosomal Proteinases
  2. Evidence That E-Box Promoter Elements and MyoD Transcription Factors Play a Role in the Induction of Cathepsin B Gene Expression during Human Myoblast Differentiation
  3. Transcription Factor Egr-1 Activates Collagen Expression in Immortalized Fibroblasts or Fibrosarcoma Cells
  4. The Receptor-Bound N-Terminal Ectodomain of the Amyloid Precursor Protein Is Associated with Membrane Rafts
  5. Evaluation of Similarities in the cis/trans Isomerase Function of Trigger Factor and DnaK
  6. The Chloroplast Import Receptor Toc34 Functions as Preprotein-Regulated GTPase
  7. cis-4-Methylsphingosine Phosphate Induces Apoptosis in Neuroblastoma Cells by Opposite Effects on p38 and ERK Mitogen-Activated Protein Kinases
  8. Evidence for the Involvement of the Unique C-Tail of S100A9 in the Binding of Arachidonic Acid to the Heterocomplex S100A8/A9
  9. The Primary Structure of Three Hemoglobin Chains from the Indigo Snake (Drymarchon corais erebennus, Serpentes): First Evidence for αD Chains and Two β Chain Types in Snakes
  10. Expression of the Tissue Kallikrein-Kinin Cascade in Granulosa Cells of the Ovary
  11. Chondroitin Sulfate Proteoglycan Is a Potent Enhancer in the Processing of Procathepsin L
  12. Tyrosinase-Mediated Oxidation of Acetaminophen to 4-Acetamido-o- Benzoquinone
  13. Properties of Baculovirus Particles Displaying GFP Analyzed by Fluorescence Correlation Spectroscopy
  14. The Promoter Context Determines Mutual Repression or Synergism between NF-κB and the Glucocorticoid Receptor
  15. Expression of Hepatocyte Growth Factor Activator Inhibitor Type 2 (HAI-2) in Human Testis: Identification of a Distinct Transcription Start Site for the HAI-2 Gene in Testis
  16. Identification and Characterization of an IgG Binding Protein in the Secretion of the Rat Coagulating Gland
  17. Characterization of Different Reactive Lysines in Bovine Heart Mitochondrial Porin
  18. Determination of the mRNA Sequence of Cathepsin Y, a Cysteine Endopeptidase from Rat Spleen, and Confirmation of Its Ubiquitous
  19. Acknowledgement
  20. Content Index
  21. Author Index
  22. Subject Index
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