Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1
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Elide A. Pastorello
Abstract
The major protein allergen of peach (Prunus persica), Pru p1, has recently been identified as a lipid transfer protein (LTP). The complete primary structure of Pru p1, obtained by direct amino acid sequence and liquid chromatography-mass spectrometry (LC-MS) analyses with the purified protein, is described here. The protein consists of 91 amino acids with a calculated molecular mass of 9178 Da. The amino acid sequence contains eight strictly conserved cysteines, as do all known LTPs, but secondary structure predictions failed to classify the peach 9 kDa protein as an ‘all-alpha type’, due to the high frequency of amino acids (nine prolines) disrupting alpha helices. Although the sequence similarity with maize LTP is only 63%, out of the 25 amino acids forming the inner surface of the tunnel-like hydrophobic cavity in maize ns-LTP 16 are identical and 7 similar in the peach homolog, supporting the hypothesis of a similar function.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Secretory Immunoglobulin A: from Mucosal Protection to Vaccine Development
- Cloning of karyopherin-α3 from Drosophila Through Its Interaction with the Nuclear Localization Sequence of Germ Cell-Less Protein
- Regulation of Bile Salt Export Pump mRNA Levels by Dexamethasone and Osmolarity in Cultured Rat Hepatocytes
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- Synthesis, Cytotoxicity and Antitumor Activity of Platinum(II) Complexes of Cyclopentanecarboxylic Acid Hydrazide
- Prion Rods Contain an Inert Polysaccharide Scaffold
- Mass Spectrometric Characterisation of Post-Translational Modification and Genetic Variation in Human Tetranectin
- Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1
- Identification of Immunoreactive Tissue Prokallikrein on the Surface Membrane of Human Neutrophils
- An Evaluation of Techniques for the Extraction and Amplification of DNA from Naturally Shed Hairs
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