Cloning of karyopherin-α3 from Drosophila Through Its Interaction with the Nuclear Localization Sequence of Germ Cell-Less Protein
-
Thomas C. Dockendorff
Abstract
The D. melanogaster germ cell-less (gcl) gene has previously been shown to play a key role in the establishment of the germ cell lineage during fly embryogenesis. To identify other molecules that function with Gcl in this process, we have conducted a yeast two-hybrid screen that utilized Gcl protein as bait. A predominant class of Gcl-interacting clones encodes a species of importin-α from Drosophila (karyopherin-α3; kap-α3), a nuclear-localization sequence binding protein previously shown to act in the transport of proteins from the cytoplasm to the nucleus. The expression of kap-α3 is widespread both temporally and spatially throughout the embryo during development, as judged by Northern blotting and whole-mount in situ hybridization to Drosophila embryos, suggesting that it functions at multiple stages of development. Studies of the Gcl/Kap-α3 interaction have identified a functional nuclear- localization sequence in Gcl protein which is necessary for an in vivo interaction and for nuclear entry of Gcl, making it likely that one role for Kap-α3 is to deliver Gcl protein to the nucleus. The identification of Kap-α3 and an in vivo substrate will allow for further characterization of the basis for specificity between importin-αmolecules and their binding substrates.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Secretory Immunoglobulin A: from Mucosal Protection to Vaccine Development
- Cloning of karyopherin-α3 from Drosophila Through Its Interaction with the Nuclear Localization Sequence of Germ Cell-Less Protein
- Regulation of Bile Salt Export Pump mRNA Levels by Dexamethasone and Osmolarity in Cultured Rat Hepatocytes
- High Activity of the Calcineurin A Subunit with a V314 Deletion
- Synthesis, Cytotoxicity and Antitumor Activity of Platinum(II) Complexes of Cyclopentanecarboxylic Acid Hydrazide
- Prion Rods Contain an Inert Polysaccharide Scaffold
- Mass Spectrometric Characterisation of Post-Translational Modification and Genetic Variation in Human Tetranectin
- Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1
- Identification of Immunoreactive Tissue Prokallikrein on the Surface Membrane of Human Neutrophils
- An Evaluation of Techniques for the Extraction and Amplification of DNA from Naturally Shed Hairs
- Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1
- Differential Regulation of Lipid and Protein Kinase Activities of Phosphoinositide 3-Kinase γ in Vitro
- The Anti-Estrogen Hydroxytamoxifen Is a Potent Antagonist in a Novel Yeast System
Articles in the same Issue
- Secretory Immunoglobulin A: from Mucosal Protection to Vaccine Development
- Cloning of karyopherin-α3 from Drosophila Through Its Interaction with the Nuclear Localization Sequence of Germ Cell-Less Protein
- Regulation of Bile Salt Export Pump mRNA Levels by Dexamethasone and Osmolarity in Cultured Rat Hepatocytes
- High Activity of the Calcineurin A Subunit with a V314 Deletion
- Synthesis, Cytotoxicity and Antitumor Activity of Platinum(II) Complexes of Cyclopentanecarboxylic Acid Hydrazide
- Prion Rods Contain an Inert Polysaccharide Scaffold
- Mass Spectrometric Characterisation of Post-Translational Modification and Genetic Variation in Human Tetranectin
- Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1
- Identification of Immunoreactive Tissue Prokallikrein on the Surface Membrane of Human Neutrophils
- An Evaluation of Techniques for the Extraction and Amplification of DNA from Naturally Shed Hairs
- Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1
- Differential Regulation of Lipid and Protein Kinase Activities of Phosphoinositide 3-Kinase γ in Vitro
- The Anti-Estrogen Hydroxytamoxifen Is a Potent Antagonist in a Novel Yeast System
