Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1
-
Hirokazu Hara
Abstract
Allograft inflammatory factor-1 (AIF-1) was initially cloned from rat cardiac allografts undergoing chronic rejection. AIF-1, possessing an EF-hand-like motif, is thought to be a Ca2+ binding protein. In this study, we identified two novel alternatively spliced variants of AIF-1 by reverse-transcription polymerase chain reaction. One variant encodes an AIF-1 protein that lacks 14 amino acids corresponding to one exon. The other variant encodes a truncated AIF-1 protein due to a frameshift introduced by an 85-bp insertion, and its C-terminal region differs from that of AIF-1. Interestingly, these variants have incomplete and no EF-hand-like motifs, respectively. The expression level of the latter variant is high in peripheral blood leukocytes, and it is selectively expressed in macrophage-like cell lines.
Copyright © 1999 by Walter de Gruyter GmbH & Co. KG
Articles in the same Issue
- Secretory Immunoglobulin A: from Mucosal Protection to Vaccine Development
- Cloning of karyopherin-α3 from Drosophila Through Its Interaction with the Nuclear Localization Sequence of Germ Cell-Less Protein
- Regulation of Bile Salt Export Pump mRNA Levels by Dexamethasone and Osmolarity in Cultured Rat Hepatocytes
- High Activity of the Calcineurin A Subunit with a V314 Deletion
- Synthesis, Cytotoxicity and Antitumor Activity of Platinum(II) Complexes of Cyclopentanecarboxylic Acid Hydrazide
- Prion Rods Contain an Inert Polysaccharide Scaffold
- Mass Spectrometric Characterisation of Post-Translational Modification and Genetic Variation in Human Tetranectin
- Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1
- Identification of Immunoreactive Tissue Prokallikrein on the Surface Membrane of Human Neutrophils
- An Evaluation of Techniques for the Extraction and Amplification of DNA from Naturally Shed Hairs
- Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1
- Differential Regulation of Lipid and Protein Kinase Activities of Phosphoinositide 3-Kinase γ in Vitro
- The Anti-Estrogen Hydroxytamoxifen Is a Potent Antagonist in a Novel Yeast System
Articles in the same Issue
- Secretory Immunoglobulin A: from Mucosal Protection to Vaccine Development
- Cloning of karyopherin-α3 from Drosophila Through Its Interaction with the Nuclear Localization Sequence of Germ Cell-Less Protein
- Regulation of Bile Salt Export Pump mRNA Levels by Dexamethasone and Osmolarity in Cultured Rat Hepatocytes
- High Activity of the Calcineurin A Subunit with a V314 Deletion
- Synthesis, Cytotoxicity and Antitumor Activity of Platinum(II) Complexes of Cyclopentanecarboxylic Acid Hydrazide
- Prion Rods Contain an Inert Polysaccharide Scaffold
- Mass Spectrometric Characterisation of Post-Translational Modification and Genetic Variation in Human Tetranectin
- Complete Amino Acid Sequence Determination of the Major Allergen of Peach (Prunus persica) Pru p1
- Identification of Immunoreactive Tissue Prokallikrein on the Surface Membrane of Human Neutrophils
- An Evaluation of Techniques for the Extraction and Amplification of DNA from Naturally Shed Hairs
- Isolation of Two Novel Alternative Splicing Variants of Allograft Inflammatory Factor-1
- Differential Regulation of Lipid and Protein Kinase Activities of Phosphoinositide 3-Kinase γ in Vitro
- The Anti-Estrogen Hydroxytamoxifen Is a Potent Antagonist in a Novel Yeast System
