10. Cholesterol in model membranes
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Abstract
Because cholesterol is an essential component of numerous biological membranes, its effect on model membranes has been an active area of research for several decades. The impact of cholesterol on the molecular order, dynamics and phase equilibria in model membranes has been studied by many experimental techniques including nuclear magnetic resonance (NMR). NMR provides quantitative information on the organization and behavior of the molecules making up the membrane and is sensitive to changes in the order and dynamics of these systems. This chapter will provide a broad review of the contributions of NMR experiments to the understanding of the behavior of cholesterol and phospholipids in model membrane systems of varying complexity.
Abstract
Because cholesterol is an essential component of numerous biological membranes, its effect on model membranes has been an active area of research for several decades. The impact of cholesterol on the molecular order, dynamics and phase equilibria in model membranes has been studied by many experimental techniques including nuclear magnetic resonance (NMR). NMR provides quantitative information on the organization and behavior of the molecules making up the membrane and is sensitive to changes in the order and dynamics of these systems. This chapter will provide a broad review of the contributions of NMR experiments to the understanding of the behavior of cholesterol and phospholipids in model membrane systems of varying complexity.
Chapters in this book
- Frontmatter I
- Preface V
- Contents IX
- List of contributing authors XIII
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Part I. Structural and dynamic characterization
- 1. Biophysical perspectives of lipid membranes through the optics of neutron and X-ray scattering 1
- 2. X-ray structure analysis of lipid membrane systems: solid-supported bilayers, bilayer stacks, and vesicles 43
- 3. Structural investigations of membrane-associated proteins by neutron reflectometry 87
- 4. Collective dynamics in model biological membranes measured by neutron spin echo spectroscopy 131
- 5. Spontaneous lipid transfer rate constants 177
- 6. Fundamentals of Nuclear Magnetic Resonance spectroscopy (NMR) and its applications 195
- 7. Collective dynamics in lipid membranes 231
- 8. Mapping protein– and peptide–membrane interactions by atomic force microscopy: strategies and opportunities 269
- 9. Imaging the distributions of lipids and proteins in the plasma membrane with high-resolution secondary ion mass spectrometry 287
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Part II. Biomimetic, biorelated, or biological systems
- 10. Cholesterol in model membranes 325
- 11. Study of mitochondrial membrane structure and dynamics on the molecular mechanism of mitochondrial membrane processes 365
- 12. Monitoring oxygen-sensitive membranes and vitamin E as an antioxidant 391
- 13. Giant vesicles: A biomimetic tool for assessing membrane material properties and interactions 415
- 14. Formation and properties of asymmetric lipid vesicles prepared using cyclodextrin-catalyzed lipid exchange 441
- 15. Application and characterization of asymmetric-supported membranes 465
- 16. Styrene-maleic acid copolymers: a new tool for membrane biophysics 477
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Part III. Molecular dynamics – simulation and theory
- 17. On the origin of “Rafts”: The plasma membrane as a microemulsion 499
- 18. Combining experiment and simulation to study complex biomimetic membranes 515
- 19. Simulations of biological membranes with the Martini model 551
- 20. Multiscale modeling of lipid membrane 569
- 21. Molecular dynamics simulation studies of small molecules interacting with cell membranes 603
Chapters in this book
- Frontmatter I
- Preface V
- Contents IX
- List of contributing authors XIII
-
Part I. Structural and dynamic characterization
- 1. Biophysical perspectives of lipid membranes through the optics of neutron and X-ray scattering 1
- 2. X-ray structure analysis of lipid membrane systems: solid-supported bilayers, bilayer stacks, and vesicles 43
- 3. Structural investigations of membrane-associated proteins by neutron reflectometry 87
- 4. Collective dynamics in model biological membranes measured by neutron spin echo spectroscopy 131
- 5. Spontaneous lipid transfer rate constants 177
- 6. Fundamentals of Nuclear Magnetic Resonance spectroscopy (NMR) and its applications 195
- 7. Collective dynamics in lipid membranes 231
- 8. Mapping protein– and peptide–membrane interactions by atomic force microscopy: strategies and opportunities 269
- 9. Imaging the distributions of lipids and proteins in the plasma membrane with high-resolution secondary ion mass spectrometry 287
-
Part II. Biomimetic, biorelated, or biological systems
- 10. Cholesterol in model membranes 325
- 11. Study of mitochondrial membrane structure and dynamics on the molecular mechanism of mitochondrial membrane processes 365
- 12. Monitoring oxygen-sensitive membranes and vitamin E as an antioxidant 391
- 13. Giant vesicles: A biomimetic tool for assessing membrane material properties and interactions 415
- 14. Formation and properties of asymmetric lipid vesicles prepared using cyclodextrin-catalyzed lipid exchange 441
- 15. Application and characterization of asymmetric-supported membranes 465
- 16. Styrene-maleic acid copolymers: a new tool for membrane biophysics 477
-
Part III. Molecular dynamics – simulation and theory
- 17. On the origin of “Rafts”: The plasma membrane as a microemulsion 499
- 18. Combining experiment and simulation to study complex biomimetic membranes 515
- 19. Simulations of biological membranes with the Martini model 551
- 20. Multiscale modeling of lipid membrane 569
- 21. Molecular dynamics simulation studies of small molecules interacting with cell membranes 603
