Article
Licensed
Unlicensed
Requires Authentication
Glucose metabolism and diabetogenic gene expression analysis of chloroform fraction of Andrographis paniculata (Nees) whole herb in diabetic albino mice
-
Anand Chaurasia
Published/Copyright:
August 10, 2012
Abstract
Exposure of rampant higher glucose level in diabetic condition could lead earlier and late diabetic complication eventually caused malign effect in body organs. The aim of the present work was to analyze the anti-diabetic potential of chloroform fraction of ethanol extract of Andrographis paniculata (AP) and diabetes laden gene expression alteration. In streptozotocin (60 mg/kg bw, i.p.) induced Type 2 diabetes albino mice, two weeks consecutive repeated dose treatment of chloroform fraction of AP at dose of 200 mg/kg, orally was evaluated for antidiabetic screening. Fasting blood glucose, oral glucose tolerance, serum lipid profile, tissues glycogen content, glucose 6-phosphatase and hexokinase enzymes level in liver, in vitro and in vivo insulin estimation were measured on last day of treatment. Subsequently presence of responsible phytoconstituents for respective activity was observed by HPLC. In diabetic mice, the fraction reduced the level of harmful cholesterol, while increased the favorable cholesterol significantly. Biochemical enzymes like glycogen, glucose-6-phosphatase and hexokinase were evaluated in body tissues. Apart from this in vitro, in vivo insulin estimation and diabetogenic gene expression analysis like GK, PEPCK, G-6pase, Glut-4, AR, PPAR-α,γ and TNF-α were evaluated using RT-PCR technique. Antidiabetic screening of fraction of AP at molecular level revealed significant antidiabetic activity.
Published Online: 2012-8-10
©2012 Walter de Gruyter GmbH & Co. KG, Berlin/Boston
You are currently not able to access this content.
You are currently not able to access this content.
Articles in the same Issue
- Research Article
- H. Spinosa T. Anders Ameliorates Diabetic Neuropathy in Wistar Albino Rats
- Anti-Inflammatory Effects of Shea Butter through Inhibition of Inos, Cox-2, and Cytokines via the Nf-Kb Pathway in Lps-Activated J774 Macrophage Cells
- Efficacy of Aphrodisiac Plants towards Improvement in Semen Quality and Motility in Infertile Males
- Hydroalcoholic extracts of Indian medicinal plants can help in amelioration from oxidative stress through antioxidant properties
- Antioxidant Potential and Protection of Pancreatic ?- cells by Calotropis gigantea in Streptozocin Induced Diabetic Rats
- Infectivity of Macrophages and the Histopathology of Cutaneous Lesions, Liver and Spleen is Attenuated by Leaf Extract of Vernonia Amygdalina in Leishmania Major Infected BALB/c Mice
- In Vitro 5-LOX Inhibitory and Antioxidant Activities of Extracts and Compounds from the Aerial Parts of Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm.
- Antihyperglycemic and Antihyperlipidemic Effects of n-hexane Fraction from the Hydro-methanolic Extract of Sepals of Salmalia malabarica in Streptozotocin-induced Diabetic Rats
- Lycopene an Adjunctive Therapy for Helicobacter Pylori Eradication: a Quasi-Control Trial
- Efficacy of Baptisia Tinctoria in the Treatment of Typhoid: Its Possible Role in Inducing Antibody Formation
- A Preliminary Study of the Mechanism of Hypoglycaemic Activity of Nauclea latifolia Leaf Ethanolic Extract
- The Microalga Spirulina platensis Presents Anti-inflammatory Action as well as Hypoglycemic and Hypolipidemic Properties in Diabetic Rats
- Evaluation of Antiulcer Potential of Mimusops hexandra in Experimental Gastro Duodenal Ulcers
- Flavonoids Purified from Parsley Inhibit Human Blood Platelet Aggregation and Adhesion to Collagen under Flow
- Potentiation of Etoposide and Temozolomide Cytotoxicity by Curcumin and Turmeric Force in Brain Tumor Cell Lines
- Glucose metabolism and diabetogenic gene expression analysis of chloroform fraction of Andrographis paniculata (Nees) whole herb in diabetic albino mice
- Anti-hyperlipidemic Activity of Cucumis Melo Fruit Peel Extracts in High Cholesterol Diet Induced Hyperlipidemia in Rats
- Sphaeranthus indicus Flower Derived Constituents Exhibits Synergistic Effect against Acetylcholinesterase and Possess Potential Antiamnestic Activity
- Comparison of effectiveness in antitumor activity between flavonoids and polyphenols of the methanolic extract of roots of Potentilla fulgens in breast cancer cells
- An Initial Investigation into the Anti-inflammatory Activity and Antioxidant Capacity of alpha-Cyclodextrin-Complexed Manuka Honey
- In vivo Antioxidant and Vasodilating Activities of Gmelina arborea (Verberaceae) Leaves Hexane Extract
- The Effects of Supplemental Fish Oil on Blood Pressure and Morning Cortisol in Normotensive Adults: a Pilot Study
- Effect of Smilax zeylanica roots and rhizomes in paracetamol induced hepatotoxicity
- The Antidiabetic Activities of the Methanolic Root Bark Extract of Afzelia africana in Alloxan-Induced Diabetic Mice
- Long-Term Treatment with a Health Product (Squina Diacylglyceryl Ether) Enhances Innate and Adaptive Immunity in Mice
- Review Article
- Glucosinolates: The Phytochemicals of Nutraceutical Importance
- Conference Presentation
- *Abstracts - 7th IN-CAM Research Symposium, Evaluating CAM Practices: Effectiveness, Integration, Economics & Safety - November 2012
- Discussion Paper
- Using Role Substitution To Address The Health Workforce Shortage And To Facilitate Integration?
- Short Communication on Medicinal Herbs
- Contractile Effect of Radish and Betel Nut Extracts on Rabbit Gallbladder
- A New Generation of Complementary Medicine Users?
- Pharmacological Evaluation of Fruits of Terminalia belerica Roxb. for Antiulcer Activity
Articles in the same Issue
- Research Article
- H. Spinosa T. Anders Ameliorates Diabetic Neuropathy in Wistar Albino Rats
- Anti-Inflammatory Effects of Shea Butter through Inhibition of Inos, Cox-2, and Cytokines via the Nf-Kb Pathway in Lps-Activated J774 Macrophage Cells
- Efficacy of Aphrodisiac Plants towards Improvement in Semen Quality and Motility in Infertile Males
- Hydroalcoholic extracts of Indian medicinal plants can help in amelioration from oxidative stress through antioxidant properties
- Antioxidant Potential and Protection of Pancreatic ?- cells by Calotropis gigantea in Streptozocin Induced Diabetic Rats
- Infectivity of Macrophages and the Histopathology of Cutaneous Lesions, Liver and Spleen is Attenuated by Leaf Extract of Vernonia Amygdalina in Leishmania Major Infected BALB/c Mice
- In Vitro 5-LOX Inhibitory and Antioxidant Activities of Extracts and Compounds from the Aerial Parts of Lopholaena coriifolia (Sond.) E. Phillips & C.A. Sm.
- Antihyperglycemic and Antihyperlipidemic Effects of n-hexane Fraction from the Hydro-methanolic Extract of Sepals of Salmalia malabarica in Streptozotocin-induced Diabetic Rats
- Lycopene an Adjunctive Therapy for Helicobacter Pylori Eradication: a Quasi-Control Trial
- Efficacy of Baptisia Tinctoria in the Treatment of Typhoid: Its Possible Role in Inducing Antibody Formation
- A Preliminary Study of the Mechanism of Hypoglycaemic Activity of Nauclea latifolia Leaf Ethanolic Extract
- The Microalga Spirulina platensis Presents Anti-inflammatory Action as well as Hypoglycemic and Hypolipidemic Properties in Diabetic Rats
- Evaluation of Antiulcer Potential of Mimusops hexandra in Experimental Gastro Duodenal Ulcers
- Flavonoids Purified from Parsley Inhibit Human Blood Platelet Aggregation and Adhesion to Collagen under Flow
- Potentiation of Etoposide and Temozolomide Cytotoxicity by Curcumin and Turmeric Force in Brain Tumor Cell Lines
- Glucose metabolism and diabetogenic gene expression analysis of chloroform fraction of Andrographis paniculata (Nees) whole herb in diabetic albino mice
- Anti-hyperlipidemic Activity of Cucumis Melo Fruit Peel Extracts in High Cholesterol Diet Induced Hyperlipidemia in Rats
- Sphaeranthus indicus Flower Derived Constituents Exhibits Synergistic Effect against Acetylcholinesterase and Possess Potential Antiamnestic Activity
- Comparison of effectiveness in antitumor activity between flavonoids and polyphenols of the methanolic extract of roots of Potentilla fulgens in breast cancer cells
- An Initial Investigation into the Anti-inflammatory Activity and Antioxidant Capacity of alpha-Cyclodextrin-Complexed Manuka Honey
- In vivo Antioxidant and Vasodilating Activities of Gmelina arborea (Verberaceae) Leaves Hexane Extract
- The Effects of Supplemental Fish Oil on Blood Pressure and Morning Cortisol in Normotensive Adults: a Pilot Study
- Effect of Smilax zeylanica roots and rhizomes in paracetamol induced hepatotoxicity
- The Antidiabetic Activities of the Methanolic Root Bark Extract of Afzelia africana in Alloxan-Induced Diabetic Mice
- Long-Term Treatment with a Health Product (Squina Diacylglyceryl Ether) Enhances Innate and Adaptive Immunity in Mice
- Review Article
- Glucosinolates: The Phytochemicals of Nutraceutical Importance
- Conference Presentation
- *Abstracts - 7th IN-CAM Research Symposium, Evaluating CAM Practices: Effectiveness, Integration, Economics & Safety - November 2012
- Discussion Paper
- Using Role Substitution To Address The Health Workforce Shortage And To Facilitate Integration?
- Short Communication on Medicinal Herbs
- Contractile Effect of Radish and Betel Nut Extracts on Rabbit Gallbladder
- A New Generation of Complementary Medicine Users?
- Pharmacological Evaluation of Fruits of Terminalia belerica Roxb. for Antiulcer Activity
