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Production and application of amylases of Rhizopus oryzae and Rhizopus microsporus var. oligosporus from industrial waste in acquisition of glucose

  • Aline Freitas EMAIL logo , Bruna Escaramboni , Ana Carvalho , Valéria Lima and Pedro Oliva-Neto
Published/Copyright: December 20, 2013
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Chemical Papers
From the journal Chemical Papers Volume 68 Issue 4

Abstract

Amylases from Rhizopus oryzae and Rhizopus microsporus var. oligosporus were obtained using agro-industrial wastes as substrates in submerged batch cultures. The enzymatic complex was partially characterised for use in the production of glucose syrup. Type II wheat flour proved better than cassava bagasse as sole carbon source for amylase production. The optimum fermentation condition for both microorganisms was 96 hours at 30°C and the amylase thus produced was used for starch hydrolysis. The product of the enzymatic hydrolysis indicated that the enzyme obtained was glucoamylase, only glucose as final product was attained for both microorganisms. R. oligosporus was of greater interest than R. oryzae for amylase production, taking into account enzyme activity, cultivation time, thermal stability and pH range. Glucose syrup was produced using concentrated enzyme and 100 g L−1 starch in a 4 hours reaction at 50°C. The bioprocess studied can contribute to fungus glucoamylase production and application.

Keywords: amylase; enzymatic hydrolysis; glucose syrup; Rhizopus; agro-industrial waste

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Published Online: 2013-12-20
Published in Print: 2014-4-1

© 2013 Institute of Chemistry, Slovak Academy of Sciences

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  3. Production and application of amylases of Rhizopus oryzae and Rhizopus microsporus var. oligosporus from industrial waste in acquisition of glucose
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  13. Synthesis of 1,1-diacetates catalysed by silica-supported boron sulfonic acid under solvent-free conditions and ambient temperature
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https://doi.org/10.2478/s11696-013-0466-x
Keywords for this article
amylase; enzymatic hydrolysis; glucose syrup; Rhizopus; agro-industrial waste

Articles in the same Issue

  1. Determination of mercury species using thermal desorption analysis in AAS
  2. Non-enzymatic hydrogen peroxide sensor based on a nanoporous gold electrode modified with platinum nanoparticles
  3. Production and application of amylases of Rhizopus oryzae and Rhizopus microsporus var. oligosporus from industrial waste in acquisition of glucose
  4. Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in Penicillium janthinellum MTCC 10889
  5. Recovery of acetaminophen from aqueous solutions using a supported liquid membrane based on a quaternary ammonium salt as ionophore
  6. Enantioseparation of mandelic acid enantiomers in ionic liquid aqueous two-phase extraction systems
  7. Fatty acid methyl ester production from acid oil using silica sulfuric acid: Process optimization and reaction kinetics
  8. Mineral constituents of edible parasol mushroom Macrolepiota procera (Scop. ex Fr.) Sing and soils beneath its fruiting bodies collected from a rural forest area
  9. Evaluation of antioxidants in Dong quai (Angelica sinensis) and its dietary supplements
  10. Electrochemical storage properties of polyaniline-, poly(N-methylaniline)-, and poly(N-ethylaniline)-coated pencil graphite electrodes
  11. Controllable one-step synthesis of ZnO nanostructures using molybdophosphoric acid
  12. I2-mediated α-selective Ferrier glycosylation approach to synthesis of O-glycosyl amino acids
  13. Synthesis of 1,1-diacetates catalysed by silica-supported boron sulfonic acid under solvent-free conditions and ambient temperature
  14. Development of oxopyrrolidine-based anti-cancer compounds: DNA binding, in silico, cell line studies, drug-likeness and mechanism at supra-molecular level
  15. Clay and charcoal composites: characterisation and application of factorial design analysis for dye adsorption
  16. Role of thermoxidation and depolymerisation in the ageing of systems paper/gum arabic/historical ink
  17. Natural organic acids promoted Beckmann rearrangement: Green and expeditious synthesis of amides under solvent-free conditions
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