Enzymatic synthesis and analytical monitoring of terpene ester by 1H NMR spectroscopy
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Dipak Patil
,
Debabrata Das
Abstract
Terpene esters of fatty acids have potential applications in food, cosmetic, and pharmaceutical industries. The present study focuses on the synthesis of terpene esters of long chain fatty acids catalyzed by Candida antarctica lipase B. Different parameters like temperature, solvent, and enzyme concentration for the esterification of terpene alcohols (geraniol and citronellol) with oleic acid were studied. Maximum conversion (98 %) was found for both terpene esters at 60°C in 2,2,4-trimethylpentane as well as in dry hexane and around 95–97 % in other tested solvents. The reaction was also carried out using stearic and linoleic acid in hexane to study the effects of unsaturation in the substrate in which stearic acid showed the maximum conversion. The reaction was monitored by 1H nuclear magnetic resonance spectroscopy. Using the peak integration values of methylene protons of terpene and terpene ester of δ = 3.6 and 4.0 for citronellol and δ = 4.2 and 4.6 for geraniol, respectively, percentage conversions of each of the esters were calculated.
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© 2010 Institute of Chemistry, Slovak Academy of Sciences
Articles in the same Issue
- Application of polyaniline as an efficient and novel adsorbent for azo dyes removal from textile wastewaters
- Enzymatic synthesis and analytical monitoring of terpene ester by 1H NMR spectroscopy
- Effect of fiber modification with carboxymethyl cellulose on the efficiency of a microparticle flocculation system
- Synthesis, crystal structure, and thermal analysis of a copper(II) complex with imidazo[4,5-f]1,10-phenantroline
- Hydrothermal synthesis of core-shell structured PS@GdPO4:Tb3+/Ce3+ spherical particles and their luminescence properties
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- Synthesis, optical, and spectroscopic characterisation of substituted 3-phenyl-2-arylacrylonitriles
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- Synthesis, characterisation, and biological activity of three new amide prodrugs of lamotrigine with reduced hepatotoxicity
- Comparative study of CTAB adsorption on bituminous coal and clay mineral
- Density of the systems (NaF/AlF3)—AlPO4 and (NaF/AlF3)—NaVO3
- Semiquinol and phenol compounds from seven Senecio species
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Articles in the same Issue
- Application of polyaniline as an efficient and novel adsorbent for azo dyes removal from textile wastewaters
- Enzymatic synthesis and analytical monitoring of terpene ester by 1H NMR spectroscopy
- Effect of fiber modification with carboxymethyl cellulose on the efficiency of a microparticle flocculation system
- Synthesis, crystal structure, and thermal analysis of a copper(II) complex with imidazo[4,5-f]1,10-phenantroline
- Hydrothermal synthesis of core-shell structured PS@GdPO4:Tb3+/Ce3+ spherical particles and their luminescence properties
- Optical characterisation of organosilane-modified nanocrystalline diamond films
- Synthesis, optical, and spectroscopic characterisation of substituted 3-phenyl-2-arylacrylonitriles
- A new group of potential antituberculotics: N-(2-pyridylmethyl)salicylamides and N-(3-pyridylmethyl)salicylamides
- Synthesis and antimicrobial properties of new 2-((4-ethylphenoxy)methyl)benzoylthioureas
- Synthesis, characterisation, and biological activity of three new amide prodrugs of lamotrigine with reduced hepatotoxicity
- Comparative study of CTAB adsorption on bituminous coal and clay mineral
- Density of the systems (NaF/AlF3)—AlPO4 and (NaF/AlF3)—NaVO3
- Semiquinol and phenol compounds from seven Senecio species
- Determination of the enthalpy of fusion of K3NbO2F4
