High level expression of the Drosophila Toll receptor ectodomain and crystallization of its complex with the morphogen Spätzle
-
Marco Stelter
, Uwe Fandrich , Kati Franzke , Angelika Schierhorn , Constanze Breithaupt , Christoph Parthier and Milton T. Stubbs
Abstract
Drosophila Toll receptors are involved in embryonic development and in the immune response of adult flies. In both processes, the Toll receptor ligand is the NGF-like cystine knot protein Spätzle. Here we present the expression of Toll receptor ectodomain in Schneider cells at high yields and demonstrate a high affinity interaction with the refolded and trypsin-processed Spätzle cystine knot domain dimer. Poorly and anisotropically diffracting crystals of the complex could be improved by deglycosylation and dehydration, paving the way for structural analyses of the Toll-Spätzle interaction.
We thank Elmar Wahle (Institut für Biochemie, Martin-Luther-Universität Halle-Wittenberg, Germany) and members of his group for useful advice concerning expression in Schneider cells. This work was supported by the Deutsche Forschungsgemeinschaft SFB610 (Protein-Zustände mit zellbiologischer und medizinischer Relevanz).
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Articles in the same Issue
- Masthead
- Masthead
- Guest Editorial
- Highlight: Protein states with cell biological and medicinal relevance
- HIGHLIGHT: PROTEIN STATES WITH CELL BIOLOGICAL AND MEDICAL RELEVANCE
- Towards improved receptor targeting: anterograde transport, internalization and postendocytic trafficking of neuropeptide Y receptors
- Progress in demystification of adhesion G protein-coupled receptors
- The unresolved puzzle why alanine extensions cause disease
- Molecular function of the prolyl cis/trans isomerase and metallochaperone SlyD
- Structure and allosteric regulation of eukaryotic 6-phosphofructokinases
- Polyionic and cysteine-containing fusion peptides as versatile protein tags
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- Oxidative in vitro folding of a cysteine deficient variant of the G protein-coupled neuropeptide Y receptor type 2 improves stability at high concentration
- Identification of prolyl oligopeptidase as a cyclosporine-sensitive protease by screening of mouse liver extracts
- In vitro maturation of Drosophila melanogaster Spätzle protein with refolded Easter reveals a novel cleavage site within the prodomain
- Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains
- High level expression of the Drosophila Toll receptor ectodomain and crystallization of its complex with the morphogen Spätzle
Articles in the same Issue
- Masthead
- Masthead
- Guest Editorial
- Highlight: Protein states with cell biological and medicinal relevance
- HIGHLIGHT: PROTEIN STATES WITH CELL BIOLOGICAL AND MEDICAL RELEVANCE
- Towards improved receptor targeting: anterograde transport, internalization and postendocytic trafficking of neuropeptide Y receptors
- Progress in demystification of adhesion G protein-coupled receptors
- The unresolved puzzle why alanine extensions cause disease
- Molecular function of the prolyl cis/trans isomerase and metallochaperone SlyD
- Structure and allosteric regulation of eukaryotic 6-phosphofructokinases
- Polyionic and cysteine-containing fusion peptides as versatile protein tags
- p0071/PKP4, a multifunctional protein coordinating cell adhesion with cytoskeletal organization
- Lysine-specific histone demethylase LSD1 and the dynamic control of chromatin
- Methylation of the nuclear poly(A)-binding protein by type I protein arginine methyltransferases – how and why
- Oxidative in vitro folding of a cysteine deficient variant of the G protein-coupled neuropeptide Y receptor type 2 improves stability at high concentration
- Identification of prolyl oligopeptidase as a cyclosporine-sensitive protease by screening of mouse liver extracts
- In vitro maturation of Drosophila melanogaster Spätzle protein with refolded Easter reveals a novel cleavage site within the prodomain
- Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains
- High level expression of the Drosophila Toll receptor ectodomain and crystallization of its complex with the morphogen Spätzle