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Polyionic and cysteine-containing fusion peptides as versatile protein tags

  • Hauke Lilie EMAIL logo , Susanne Richter , Sabine Bergelt , Stefan Frost and Franziska Gehle
Published/Copyright: April 27, 2013

Abstract

In response to advances in proteomics research and the use of proteins in medical and biotechnological applications, recombinant protein production and the design of specific protein characteristics and functions has become a widely used technology. In this context, protein fusion tags have been developed as indispensable tools for protein expression, purification, and the design of functionalized surfaces or artificially bifunctional proteins. Here we summarize how positively or negatively charged polyionic fusion peptides with or without an additional cysteine can be used as protein tags for protein expression and purification, for matrix-assisted refolding of aggregated protein, and for coupling of proteins either to technologically relevant matrices or to other proteins. In this context we used cysteine-containing polyionic fusion peptides for the design of immunotoxins. In general, polyionic fusion tags can be considered as a multifunctional module in protein technology.


Corresponding author: Hauke Lilie, Institut für Biochemie und Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany

This work was supported by the Deutsche Forschungsgemeinschaft (DFG) as part of the Sonderforschungsbereich SFB 610. We thank Gary Sawers for critically reading the manuscript.

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Received: 2013-1-15
Accepted: 2013-4-24
Published Online: 2013-04-27
Published in Print: 2013-08-01

©2013 by Walter de Gruyter Berlin Boston

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