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Fatty acids and amino acids of entomopathogenic fungus Conidiobolus coronatus grown on minimal and rich media

  • Marek Gołębiowski EMAIL logo , Aleksandra Ostachowska , Monika Paszkiewicz , Mieczysława I. Boguś , Emilia Włóka , Marta Ligęza-Żuber and Piotr Stepnowski
Published/Copyright: June 25, 2016
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Chemical Papers
From the journal Chemical Papers Volume 70 Issue 10

Abstract

Entomopathogenic fungi are referred to as potential candidates as insect pest control agents. The objective of the study was to identify fatty acids and amino acids from Conidiobolus coronatus cultured on two different media. Each medium was extracted with ethyl acetate and its mixtures with isopropanol, acetonitrile and methanol. Analyses of fatty acids and amino acids of entomopathogenic fungus C. coronatus were performed by means of gas chromatography coupled with mass spectrometry. The analysis showed that the fungus C. coronatus produces the following groups of compounds: fatty acids and amino acids; α- and β-glucopyranose were also identified. The identified fatty acids included 12-20, 22 and 24 carbon atoms per chain. The highest content of fatty acids was detected in a mycelium sample cultured in a liquid minimal medium extracted with ethyl acetate. The lowest content of these organic compounds was identified in mycelium cultured in a liquid nutrient-rich medium extracted with ethyl acetate-methanol mixture. Fatty acids were found to account for 62.0 mass % to 94.4 mass % of all organic compounds in the analyzed mycelia. C18:1 acids were detected in the highest amounts when ethyl acetate was used as the extracting agent. The identified amino acids accounted for 4 mass % to 21 mass % of all organic compounds. Upon extraction of C. coronatus mycelium samples with the ethyl acetate-methanol mixture, two anomeric forms of glucose were also identified. An analysis of the studied material confirmed, that the entomopathogenic fungus C. coronatus is a very rich source of organic compounds, which might encourage its further research so as to identify an even larger number of compounds being produced by this species.

Keywords: GC-MS; fatty acids; amino acids; Conidiobolus coronatus

Acknowledgements

The authors would like to express their gratitude to Paulina Rybicka for her assistance. This study was funded by the Polish Ministry of Science and Higher Education (grants number UMO-2011/01/D/NZ6/03114 and DS 530-8617-D-594-16).

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Received: 2015-11-19
Revised: 2016-2-12
Accepted: 2016-2-25
Published Online: 2016-6-25
Published in Print: 2016-10-1

© 2016 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2016-0067
Keywords for this article
GC-MS; fatty acids; amino acids; Conidiobolus coronatus

Articles in the same Issue

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  2. Simultaneous analysis of polar and non-polar components of cell membrane phospholipids by GC-MS
  3. Original Paper
  4. Cloud point extraction of disulfiram for its HPLC-MS/MS determination in synthetic urine
  5. Original Paper
  6. Revealing the seed proteome of the health benefitting grain amaranth (Amaranthus cruentus L.)
  7. Original Paper
  8. Possible role of hydrolytic enzymes (Sap, Kex2) in Candida albicans response to aromatic compounds bearing a sulfone moiety
  9. Original Paper
  10. Using nutritional and oxidative stress to increase content of healthbeneficial fatty acids in oleaginous and non-oleaginous yeasts
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