Chemical composition and antioxidant activity of some important betel vine landraces
-
Suryasnata Das
Abstract
The essential oil composition and in vitro antioxidant activity of eight important betel vine landraces, such as Balia, Chandrakala, Dandabalunga, Desibangla, Godibangla, Karpada local, Maghai and Nahua were evaluated in this research. GC and GC-MS analysis of the essential oil resulted in identification of 50 compounds representing 95.97% of the oil. Major compounds were eugenol, terpinolene, terpinene, cadinene, caryophyllene, 3-carene, etc. The plant essential oil was also subjected to screening for the evaluation of their antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl assay. The highest antioxidant activity was shown by Balia landraces, whereas the lowest activity by Godibangla landraces. Essential oil showed a great variety of compounds in their chemical composition and antioxidant activity. This might be due to the affect of different environmental factors and soil nutrients of betel vine cultivating zones. The GC-MS chromatogram with antioxidant activity of the above betel vine landraces would not only help to select the elite chemotypes, but also to enhance their future industrial applications.
Acknowledgements
The authors are grateful to Prof. Dr. S.C. Si, Dean, Centre of Biotechnology, and Prof. Dr. M.R. Nayak, President, Siksha ‘O’ Anusandhan University, Orisha, India, for providing facilities and encouraging throughout.
References
Abrahim N.N., Kanthimathi M.S. & Abdul-Aziz A. 2012. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase. BMC Comp. Alter. Med. 12:220.10.1186/1472-6882-12-220Search in Google Scholar PubMed PubMed Central
Chakraborty D. & Shah B. 2011. Antimicrobial, antioxidative and antihemolytic activity of Piper betel leaf extracts. Int. J. Pharm. Pharmac. Sci. 3(Suppl. 3):192–199.Search in Google Scholar
Dasgupta N. & De B. 2004. Antioxidant activity of Piper betle L. leaf extract in vitro. Food Chem. 88:219–224.10.1016/j.foodchem.2004.01.036Search in Google Scholar
Guha P. 2006. Betel leaf: the neglected green gold of India. J. Hum. Ecol. 19:87–93.10.1080/09709274.2006.11905861Search in Google Scholar
Gundala S.R., Yang C., Mukkavilli R., Paranjpe R., Brahmbhatt M., Pannu V., Cheng A., Reid M.D. & Aneja R. 2014. Hydroxychavicol, a betel leaf component, inhibits prostrate cancer through ROS-driven DNA damage and apoptosis. Toxicol. Appl. Pharmacol. 280:86–96.10.1016/j.taap.2014.07.012Search in Google Scholar PubMed PubMed Central
Hassiotis C.N., Ntana F., Lazari D.M., Poulios S. & Vlachonasios K.E. 2014. Environmental and developmental factors affect essential oil production and quality of Lavandula angustifolia during flowering period. Ind. Crops Prod. 62:359–366.10.1016/j.indcrop.2014.08.048Search in Google Scholar
Jane N.S., Deshmukh A.P. & Joshi M.S. 2014. Review of study of different diseases on betelvine plant and control measure. Int. J. Appl. Innov. Engin. Manag. 3:560–563.Search in Google Scholar
Lei D., Chan C.P., Wang Y.J., Lin B.R., Huang C.H., Lee J.J., Chen H.M. & Jeng J.H. 2003. Antioxidative and antiplatelet effects of aqueous inflorescence Piper betle extract. J. Agric. Food Chem. 51:2083–2088.10.1021/jf0210223Search in Google Scholar PubMed
Martin P. & Chang X. 2013. Production potential and crop agronomy of sweet gale (Myrica gale L.) in the north of Scotland. Ind. Crops Prod. 46:39–49.10.1016/j.indcrop.2012.12.034Search in Google Scholar
Misra P., Kumar A., Khare P., Gupta S., Kumar N. & Dube A. 2009. Pro-apoptotic effect of the landrace Bangla Mahoba of Piper betle on Leshmania donovani may be due to the high content of eugenol. J. Med. Microbiol. 58:1058–1066.10.1099/jmm.0.009290-0Search in Google Scholar PubMed
Thurairajah N. & Rahim Z.H.A. 2007. The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans. Amer. J. Biotechnol. Biochem. 3:10–15.10.3844/ajbbsp.2007.10.15Search in Google Scholar
Rahimmalek M., Mirzakhani M. & Pirbalouti A.G. 2013. Essential oil variation among 21 wild myrtle (Myrtus communis L.) populations from different geographical regions in Iran. Ind. Crops Prod. 51:328–333.10.1016/j.indcrop.2013.09.010Search in Google Scholar
Rathee J.S., Patro B.S., Mula S., Gamre S. & Chattopadhyay S. 2006. Antioxidant activity of Piper betel leaf extract and its constituents. J. Agric. Food Chem. 54:9046–9054.10.1021/jf061679eSearch in Google Scholar
Rawat A.K.S., Tripathi R.D., Khan A.J. & Balsubrahmanyam V.R. 1989. Essential oil components as markers for identification of Piper betle L. cultivars. Biochem. Syst. Ecol. 17:38–55.10.1016/0305-1978(89)90039-2Search in Google Scholar
Salleh M.N., Runnie I., Roach P.D., Suhailla-Mohamed & Abeywardena M.Y. 2002. Inhibition of low-density lipoprotein oxidation and up-regulation of low density lipoprotein receptor in HepG2 cells by tropical plant extracts. J. Agric. Food Chem. 50:3693–3697.10.1021/jf011593fSearch in Google Scholar PubMed
Sangwan N.S., Farooqi A.H.A., Shabih F. & Sangwan R.S. 2001. Regulation of essential oil production in plants. Plant Growth Reg. 34:3–21.10.1023/A:1013386921596Search in Google Scholar
Saxena M., Khare N.K., Saxena P., Syamsundar K.V. & Srivastava S.K. 2014. Antimicrobial activity and chemical composition of leaf oil in two varieties of Piper betle from northern plains of India. J. Sci. Ind. Res. 73:95–99.Search in Google Scholar
Sazwi N.N., Nalina T. & Rahim Z.H.A. 2013. Antioxidant and cytoprotective activities of Piper betle, Areca catechu, Uncaria gambir and betel quid with and without calcium hydroxide. BMC Comp. Alt. Med. 13:351.10.1186/1472-6882-13-351Search in Google Scholar PubMed PubMed Central
Shah G.A., Islam T., Sharma E., Telang S. & Malla B.S. 2013. Assessment of preliminary phytochemical screening and antioxidant potential of different extracts of Piper betleL. leaves. Int. J. Pharm. Sci. Rev. Res. 20:189–192.Search in Google Scholar
Shivashankara K.S., Roy T.K. & Geetha G.A. 2012. Antioxidant capacity, radical scavenging ability, total phenols and flavonoids in three types of betelvine (Piper betleL.) J. Spices Arom. Crops 21:64–67.Search in Google Scholar
Singtongratana N., Vadhanasin S. & Singkhonrat J. 2013. Hydroxychavicol and eugenol profiling of betel leaves from Piper betle L. obtained by liquid-liquid extraction and supercritical fluid extraction. Kasetsart J. (Nat. Sci.) 47:614–623.Search in Google Scholar
Sugumaran M., Suresh Gandhi M., Sankarnarayanan K., Yokesh M., Poornima M. & Rajasekhar S.R. 2011. Chemical composition and antimicrobial activity of vellaikodi variety of Piper betle Linn leaf oil against dental pathogens. Int. J. Pharm. Tech. Res. 4:2135–2139.Search in Google Scholar
Suppakul P., Ead N.S. & Phoopuritham P. 2006. Antimicrobial and antioxidant activities of betel oil. Kasetsart J. (Nat. Sci.) 40:91–100.Search in Google Scholar
- Abbreviations
- DPPH
2,2-diphenyl-1-picrylhydrazyl.
© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
Articles in the same Issue
- Cellular and Molecular Biology
- Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia
- Cellular and Molecular Biology
- Application of violet pigment from Chromobacterium violaceum UTM5 in textile dyeing
- Cellular and Molecular Biology
- Chemical composition and antioxidant activity of some important betel vine landraces
- Cellular and Molecular Biology
- Development of conventional and real time PCR assay for detection and quantification of Rhizoctonia solani infecting pulse crops
- Botany
- Somatic embryogenesis and in vitro shoot propagation of Gentianautriculosa
- Botany
- Effect of cold treatment on germination of Saxifraga aizoides and S. paniculata: a comparison of central (eastern Alps) and southern populations (northern Apennines)
- Botany
- Environmental factors and phytohormones enhancing expression of α-momorcharin gene in Momordica charantia
- Botany
- Unveiling the kinematics of the avoidance response in maize (Zen mays) primary roots
- Zoology
- Review of the species allocated to the genus Satchellius (Oligochaeta: Lumbricidae) with description of a new species
- Cellular and Molecular Biology
- Cation metals specific hemocyanin exhibits differential antibacterial property in mud crab, Scylla serrata
- Zoology
- Dynamics of soil Collembola communities (Hexapoda: Collembola) along the mesoclimatic gradient in a deep karst valley
- Zoology
- Hidden invertebrate diversity – phytotelmata in Bromeliaceae from palm houses and florist wholesalers (Poland)
- Zoology
- Species composition of mosquitoes (Diptera: Culicidae) in relation to climate conditions in South-Eastern Slovakia
- Zoology
- Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications
- Zoology
- Trace element enrichment in the eggshells of Grus japonensis and its association witheggshell thinningin ZhalongWetland (Northeastern China)
Articles in the same Issue
- Cellular and Molecular Biology
- Antimicrobial resistance and molecular characterisation of human campylobacters from Slovakia
- Cellular and Molecular Biology
- Application of violet pigment from Chromobacterium violaceum UTM5 in textile dyeing
- Cellular and Molecular Biology
- Chemical composition and antioxidant activity of some important betel vine landraces
- Cellular and Molecular Biology
- Development of conventional and real time PCR assay for detection and quantification of Rhizoctonia solani infecting pulse crops
- Botany
- Somatic embryogenesis and in vitro shoot propagation of Gentianautriculosa
- Botany
- Effect of cold treatment on germination of Saxifraga aizoides and S. paniculata: a comparison of central (eastern Alps) and southern populations (northern Apennines)
- Botany
- Environmental factors and phytohormones enhancing expression of α-momorcharin gene in Momordica charantia
- Botany
- Unveiling the kinematics of the avoidance response in maize (Zen mays) primary roots
- Zoology
- Review of the species allocated to the genus Satchellius (Oligochaeta: Lumbricidae) with description of a new species
- Cellular and Molecular Biology
- Cation metals specific hemocyanin exhibits differential antibacterial property in mud crab, Scylla serrata
- Zoology
- Dynamics of soil Collembola communities (Hexapoda: Collembola) along the mesoclimatic gradient in a deep karst valley
- Zoology
- Hidden invertebrate diversity – phytotelmata in Bromeliaceae from palm houses and florist wholesalers (Poland)
- Zoology
- Species composition of mosquitoes (Diptera: Culicidae) in relation to climate conditions in South-Eastern Slovakia
- Zoology
- Effect of supplemental feeds on liver and intestine of common carp (Cyprinus carpio) in semi-intensive rearing system: histological implications
- Zoology
- Trace element enrichment in the eggshells of Grus japonensis and its association witheggshell thinningin ZhalongWetland (Northeastern China)
