Me11b receptor mediated action of melatonin in regulation of lung associated immune system (LAIS) of Perdicula asiatica: An in vitro study
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Rajesh Kumar Kharwar
,
Chandana Haldar
Abstract
The pineal hormone melatonin plays an important role in immunomodulation of avian species. These immunomodulatory effects of melatonin are known to be mediated by three membrane bound receptors found in birds, Mel1a, Mel1b and Mel1c- However, specific involvement of these receptors in mediating immunomodulatory effects of melatonin in Lung Associated Immune System (LAIS) remains unexplored till date. The aim of the present study was to check the specific melatonin receptor subtype involved in regulation of LAIS. For this purpose, we used luzindole, a non selective and 4P-PDOT, a selective Mel1b receptor antagonist under in vitro condition to assess their specific potency towards melatonin induced cellular immunity in the lung tissue of Perdicula asiatica. Melatonin enhanced proliferation of lymphocytes isolated from lung tissue and up-regulated both receptor types (Mel1a and Mel1b) on physiological doses while higher doses decreased the proliferation and down regulated the expression of both receptors. Luzindole decreased proliferation of lung lymphocytes and down-regulated the expression of both receptors in a dose dependent manner while 4P-PDOT decreased proliferation of lymphocytes isolated from lung and down-regulated the expression of Mel1b receptor subtype only. Proliferation rate of lymphocytes isolated from lung tissue followed more the expression pattern of Mel1b than the Mel1a. Thus, we may suggest that Mel1b might be involved in cell mediated immunity in lung of P. asiatica to a greater extent than Mel1a.
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- A new method for estimating soil water repellency index
- Particle-size and organic matter effects on structure and water retention of soils
- Depth-dependent heterogeneity of water flow in sandy soil under grass
- Spatial variability of hydrophysical properties of fallow sandy soils
- Effects of vegetation at different succession stages on soil properties and water flow in sandy soil
- Impact of crop residue retention and tillage on water infiltration into a water-repellent soil
- Night-time leaf wetting process and its effect on the morning humidity gradient as a driving force of transpirational water loss in a semi-arid cornfield
- Hoplophthiracarus species (Acari: Oribatida: Phthiracaridae) from China with descriptions of two new species
- A new species of Trhypochthoniellus (Acari: Oribatida: Trhypochthoniidae) from Chile, with remarks on diagnosis of the genus
- New species of oribatid mites (Acari: Oribatida) of the genera Austrachipteria (Achipteriidae), Cultroribula (Astegistidae) and Microlamellarea (Lamellareidae) from New Zealand
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