Study of deoxynivalenol effect on metallothionein and glutathione levels, antioxidant capacity, and glutathione-S-transferase and liver enzymes activity in rats
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Pavlina Sobrova
,
Anna Vasatkova
Abstract
Deoxynivalenol (DON, vomitoxin) is one of several human mycotoxins acquired through residues in foods of plant origin (cereals) and through foods of animal origin (kidneys, liver, milk, eggs). The aim of this study was to investigate the impact of deoxynivalenol-contaminated mouldy grain on the health status of rats and their ability to resist oxidative stress. The rats were fed a diet supplemented with DON along with organic and inorganic Zn(II), and vitamins for 28 days. The study focuses particularly on protective mechanisms and levels of reduced glutathione and metallothioneins (MT) against the adverse effects of xenobiotics. The highest concentrations of MT were detected in the tissues of kidneys ((6.70 ± 0.05) μg g−1) and liver ((6.00 ± 0.05) μg g−1), both of which are involved in detoxifying cationic xenobiotics. Lower concentrations were also detected in heart, brain, testes, and muscle tissues (approximately 3.0 μg g−1). Antioxidant capacity, glutathione-S-transferase, and liver enzymes activity were also studied. In conclusion, MT may play an important role in the detoxification of mycotoxins. Even though its role is not fully understood, MT are crucial for the redistribution of ions important for transcription factors and interaction with reactive oxygen species that form mycotoxins.
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© 2012 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Quantitative analysis of two adulterants in Cynanchum stauntonii by near-infrared spectroscopy combined with multi-variate calibrations
- Study of deoxynivalenol effect on metallothionein and glutathione levels, antioxidant capacity, and glutathione-S-transferase and liver enzymes activity in rats
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- Relationship between the decrease of degree of polymerisation of cellulose and the loss of groundwood pulp paper mechanical properties during accelerated ageing
- Improved hydrothermal synthesis of MoS2 sheathed carbon nanotubes
- Fabrication of a micro-direct methanol fuel cell using microfluidics
- Determination of pK a of benzoic acid- and p-aminobenzoic acid-modified platinum surfaces by electrochemical and contact angle measurements
- Theoretical enthalpies of formation and structural characterisation of halogenated nitromethanes and isomeric halomethyl nitrites
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