Preparation and preliminary biological evaluation of radiogallium-labeled DTPA-amlodipine complex for possible L-type calcium channel imaging
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Tahereh Firuzyar
,
Yousef Fazaeli
Abstract
A DTPA-conjugated amlodipine analog (DTPA-AMLO) 3, was prepared for possible voltage gated calcium channel imaging after radiolabeling with Ga-67. [67Ga]-DTPA-AMLO complex was prepared starting [67Ga]gallium chloride and DTPA-AMLO in 60–90 min at 50–60 ℃ in phosphate buffer. The partition co-efficient and stability of the tracer was determined in final solution (25 ℃) and presence of human serum (37 ℃) up to 24 h. The biodistribution of the labeled compound in wild-type rats were determined up to 72 h using organ counting and SPECT. The radiolabled complex was prepard in high radiochemical purity (>96%, RTLC and >98% HPLC) and significant specific activity (7–10 GBq/mmol). The log P for the complex was calculated as − 0.594, consistent with a water soluble complex. The tracer is mostly washed out through kidneys which were in full compliance with the amlodipine metabolism and imaging studies demonstrated the same behavior. The tracer uptake in organs with smooth muscles was observed in stomach, colon as well as intestine.
©2014 Walter de Gruyter Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Nature of nano-sized plutonium particles in soils at the Hanford Site
- Studies on electrochemical behaviour on NpO22+/NpO2+ redox couple at single walled carbon nanotube modified glassy carbon electrode (SWCNT-GC)
- Study on the technology of U-Pu Co-stripping process for reprocessing spent fuel of fast reactor
- Use of the transpiration method to study polonium evaporation from liquid lead-bismuth eutectic at high temperature
- In-situ formation, thermal decomposition, and adsorption studies of transition metal carbonyl complexes with short-lived radioisotopes
- Production of 111In and 114mIn by proton induced reactions: an update on excitation functions, chemical separation – purification and recovery of target material
- Preparation and preliminary biological evaluation of radiogallium-labeled DTPA-amlodipine complex for possible L-type calcium channel imaging
- An improved analytical method for iodine-129 determination in low-level radioactive waste
Artikel in diesem Heft
- Frontmatter
- Nature of nano-sized plutonium particles in soils at the Hanford Site
- Studies on electrochemical behaviour on NpO22+/NpO2+ redox couple at single walled carbon nanotube modified glassy carbon electrode (SWCNT-GC)
- Study on the technology of U-Pu Co-stripping process for reprocessing spent fuel of fast reactor
- Use of the transpiration method to study polonium evaporation from liquid lead-bismuth eutectic at high temperature
- In-situ formation, thermal decomposition, and adsorption studies of transition metal carbonyl complexes with short-lived radioisotopes
- Production of 111In and 114mIn by proton induced reactions: an update on excitation functions, chemical separation – purification and recovery of target material
- Preparation and preliminary biological evaluation of radiogallium-labeled DTPA-amlodipine complex for possible L-type calcium channel imaging
- An improved analytical method for iodine-129 determination in low-level radioactive waste
