Modification of natural pigskin collagen via cryogrinding: a focused study on its physiochemical properties
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Yuling Xu
,
Lang He
,
Chengzhi Xu
Abstract
Natural pigskin was subjected to cryogrinding before extraction, and effects of the approach on extraction rate, structure, and properties of collagen were prospected systematically. It was found that the extraction rate multiplied gradually from 22% to 40% with an extended grinding duration from 0 to 20 min. Compared with natural collagen, the ground one soared by about 80% concerning the net yield. Electrophoresis revealed the stereo structures of the extracted collagen were not destroyed when ground, while a small amount of it degraded accordingly, whose conclusion was further corroborated by circular dichroism (CD) and infrared spectrometry. Results from contact angle (CA) test clarified that the hydrophilicity of collagen enhanced with prolonged grinding. Moreover, analysis of fibrillogenesis behavior verified that, after grinding, the assembly rate for collagen in the turbidity assay dented with a lengthened equilibrium time; finer fibril network with larger pore size and weakened elasticity was later observed. Methyl thiazolyl tetrazolium (MTT) analysis manifested that ground collagen was more conducive to cell proliferation. This polymer processing approach not only provides us with a facile approach to manipulate capacities of collagen but also sheds light on other potential substances beneath the same principle.
Funding source: Application Foundation Frontier Project of Wuhan Science and Technology Bureau
Award Identifier / Grant number: 2019020701011478
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 21676208
Award Identifier / Grant number: 21706201
Award Identifier / Grant number: 22178277
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Research ethics: The animal trial was conducted according to the Animal Scientific Procedures Act 1986 (Home Office Code of Practice. HMSO: London January 1997) and EU regulations (Directive 2010/63/EU). The whole procedure was approved by the Animal Care and Use Committee of Wuhan Polytechnic University (Wuhan, China).
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: This study was supported by the National Natural Science Foundation of China (nos. 21676208, 21706201, 22178277), and Application Foundation Frontier Project of Wuhan Science and Technology Bureau (no. 2019020701011478).
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Conflict of interest statement: The authors declare that they have no conflicts of interest regarding this article.
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Supplementary Material
This article contains supplementary material (https://doi.org/10.1515/polyeng-2022-0269).
© 2023 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Material Properties
- Modification of natural pigskin collagen via cryogrinding: a focused study on its physiochemical properties
- Effects of nano-silica on the crystallization, structure, and mechanical properties of crosslinked ethylene-octene copolymer/nano-silica composites
- Preparation and Assembly
- Microencapsulation of polymeric phase change materials (MPCM) for thermal energy storage in industrial coating applications
- A waterborne uniform graphene oxide-epoxy complex with enhanced anticorrosive properties enabled by intercalation polymerization
- Engineering and Processing
- Development of a methodical approach to set-up the injection velocity profile dependent on the part geometry
- Development of variotherm extrusion blow molding technology to produce high-gloss automotive spoilers
- Fabrication, property characterization, and benefit analysis of mixing mechanism of nitrogen and melt, and its comparison of the porous-foam polypropylene injection molding parts
Artikel in diesem Heft
- Frontmatter
- Material Properties
- Modification of natural pigskin collagen via cryogrinding: a focused study on its physiochemical properties
- Effects of nano-silica on the crystallization, structure, and mechanical properties of crosslinked ethylene-octene copolymer/nano-silica composites
- Preparation and Assembly
- Microencapsulation of polymeric phase change materials (MPCM) for thermal energy storage in industrial coating applications
- A waterborne uniform graphene oxide-epoxy complex with enhanced anticorrosive properties enabled by intercalation polymerization
- Engineering and Processing
- Development of a methodical approach to set-up the injection velocity profile dependent on the part geometry
- Development of variotherm extrusion blow molding technology to produce high-gloss automotive spoilers
- Fabrication, property characterization, and benefit analysis of mixing mechanism of nitrogen and melt, and its comparison of the porous-foam polypropylene injection molding parts
