Preventive effects of the methanol soluble fraction of Millettia macrophylla Benth (Fabaceae) on an osteoporosis-like model of ovariectomized Wistar rats
-
Stéphane Zingue
,
Marie Alfrede Mvondo
Abstract
Background: Millettia macrophylla Benth is a Cameroonian medicinal plant traditionally used to alleviate menopause-related problems. The methanol soluble fraction of this plant was shown to exhibit estrogenic effects in vitro in Human Embryonic kidney cells, and in vivo on ovariectomized rat following the classical uterotrophic assay. Since estrogens have been involved in bone remodeling process, the present study then aimed at evaluating bone loss preventive effects of the methanol soluble fraction of Millettia macrophylla (MM-met) in ovariectomized rat model.
Methods: Twenty-five healthy Wistar female rats (3-month-old) were randomly assigned to a sham-operated group and to four treated ovariectomized (OVX) groups. Treatments lasted 8 weeks and animals were sacrificed. The uterus, the femoral and the tibia bones of each animal were collected, weighed and fixed in 10% formalin for histological analysis.
Results: Results showed that ovariectomy decreased uterine wet weight (p<0.01), induced body weight gain (p<0.01), decreased both femoral and tibia bone density and mineral content and increased alkaline phosphatase activity (p<0.05). E2V and MM-met treatments in general prevented bone mass loss and/or bone density loss. At all tested doses, MM-met induced a significant decrease of alkaline phosphatase activity (p<0.05). As observed with E2V, MM-met also induced a significant protective effect on bone, and this was indicated by an abundance of bone marrow in an almost intact trabecular network.
Conclusions: The overall results show that the methanol soluble fraction of Millettia macrophylla may prevent ovariectomy-induced bone mass loss and deterioration of the trabecular microarchitecture.
Acknowledgments
Special thanks to the German Academic Exchange Service (DAAD) and the Alexander von Humboldt Foundation for support.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article. Research support played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared
References
1. DealC. Potential new drug targets for osteoporosis. Nat Clin Pract Rheumatol2009;5:20–7.10.1038/ncprheum0977Suche in Google Scholar PubMed
2. HazaveheiSM, TaghdisiMH, SaidiM. Application ofthe health belief model for osteoporosis prevention among middle school girl students, Garmsar, Iran. Educ Health2007;20:1–11.Suche in Google Scholar
3. CooperC, AtkinsonEJ, JacobsenSJ, O’FallonWM,Melton III LJ. Population-based study of survivalafter osteoporotic fractures. Am J Epidemiol1993;137:1001–5.10.1093/oxfordjournals.aje.a116756Suche in Google Scholar PubMed
4. JohnellO. Advances in osteoporosis: better identification of risk factors can reduce morbidity and mortality. J Intern Med1996;239:229–304.10.1046/j.1365-2796.1996.429781000.xSuche in Google Scholar PubMed
5. SitiNF, ShahrumA, NorazlinaM, AhmadNS. Labisia pumila prevents complications of osteoporosis by increasing bone strength in a rat model of post-menopausal osteoporosis. Evid-Based Compl Alt2012. DOI:10.1155/2012/948080.Suche in Google Scholar
6. FordhamJN. Osteoporosis: all your questions answered. London, UK: Churchill Living Stone, 2004.Suche in Google Scholar
7. International Osteoporosis Foundation. Facts and statistics about osteoporosis and its impact. J Bone Miner Res2009;4:113–18.Suche in Google Scholar
8. Al-AnaziAF, QureshiVF, JavidK, QureshiS. Preventive effects of phytoestrogens against post-menopausal osteoporosis as compared to the available therapeutic choices: an overview. J Nat Sci Biol Med2011;2:154–63.10.4103/0976-9668.92322Suche in Google Scholar PubMed PubMed Central
9. Al-AzzawiF. Prevention of postmenopausal osteoporosis and associated fractures: clinical evaluation of the choice between estrogen and bisphosphonates. Gynecol Endocrinol2008;24:601–9.10.1080/09513590802296245Suche in Google Scholar PubMed
10. ArcangeloVP, PetersonAM. Pharmacotherapeutics for advanced practice: a practical approach, 2nd ed. Philadelphia, PA: Lippincott Williams & Wilkins, 2005.Suche in Google Scholar
11. RaiszLG. Pathogenesis of osteoporosis: concepts, conflicts, and prospects. J Clin Invest2005;115:3318–25.10.1172/JCI27071Suche in Google Scholar PubMed PubMed Central
12. RossouwJE, AndersonGL, PrenticeRL, LaCroixAZ, KooperbergC, StefanickML, et al. Writing group for the women’s health initiative investigators. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results from the women’s health initiative randomized controlled trial. J Am Med Assoc2002;288:321–33.10.1001/jama.288.3.321Suche in Google Scholar PubMed
13. SperoffL, FritzMA. Clinical Gynecologic Endocrinology and Infertility, 7th ed.Philadelphia: Lippincott Williams & Wilkins, 2005; 897, 900–2, 912, 916.Suche in Google Scholar
14. North American Menopause Society. Position statement: estrogen and progestogen use in peri- and postmenopausal women: March 2007 position statement of the North American menopause society. Menopause2007;14:168–82.10.1097/gme.0b013e31803167abSuche in Google Scholar PubMed
15. PitkinJ. Alternative and complementary therapies for menopause. Menopause Int2012;18:20–7.10.1258/mi.2012.012001Suche in Google Scholar PubMed
16. World Health Organization. Traditional medicine. Fact sheet 134. 2003-5. Archived from the original on 2008-07-28, 2008.Suche in Google Scholar
17. BanzouziJT, ProstA, RajemiarimiraboM, OngokaP. Traditional uses of African Millettia species (Fabaceae). Int J Bot2008;4:406–20.10.3923/ijb.2008.406.420Suche in Google Scholar
18. LawalIO, UzokweNE, IgboanugoABI, AdioAF, AwosanEA, NwogwugwuJO. Ethno medicinal information on collation and identification of some medicinal plants in research institutes of south-west Nigeria. Afr J Pharm Pharmacol2010;4:1–17.Suche in Google Scholar
19. ZingueS, NjamenD, TchoumtchouaJ, HalabalakiM, SimpsonE, ClyneC, et al. Effects of Millettia macrophylla (Fabaceae) extracts on estrogen target organs of female Wistar rat. J Pharmacol Sci2013;123:120–31.10.1254/jphs.13094FPSuche in Google Scholar
20. AhmadNS, LeongLP, NorlizaM, NorazlinaM, ImaNS. The effects of Labisia pumila var. Alata on bone markers and bone calcium in a rat model of post-menopausal osteoporosis. J Ethnopharmacol2011;133:538–42.10.1016/j.jep.2010.10.033Suche in Google Scholar
21. FestingMF. Suitability of the rat for different investigations. In: AltmanPL, KatzDD, editors. Inbred and genetically defined strains of laboratory animals, part I, mouse and rat. Bethesda, MD: Federation of American Societies for Experimental Biology, 1979:237–8.Suche in Google Scholar
22. LaneNE, YaoW, KinneyJH, ModinG, BaloochM, WronskiTJ. Both hPTH (1–34) and bFGF increase trabecular bone mass in osteopenic rats but they have different effects on trabecular bone architecture. J Bone Miner Res2003;18:2105–15.10.1359/jbmr.2003.18.12.2105Suche in Google Scholar
23. Ima-NirwanaS, NorazlinaM, KhalidBA. Pattern of bone mineral density in growing male and female rats after gonadectomy. J ASEAN Fed Endocr Soc1998;16:21–36.Suche in Google Scholar
24. Yoon-BokL, HyongJ, KangSK, Jae-YongL, Sang-YoonN, Sang-HeeC, et al. Evaluation of the preventive effect of isoflavone extract on bone loss in ovariectomized rats. Biosci Biotechnol Biochem2004;68:1040–5.10.1271/bbb.68.1040Suche in Google Scholar
25. LucindaLM, VieiraBJ, OliveiraTT, SáRC, PetersVM, ReisJE, et al. Evidences of osteoporosis improvement in Wistar rats treated with Ginkgo biloba extract: a histomorphometric study of mandible and femur. Fitoterapia2010;81:982–7.10.1016/j.fitote.2010.06.014Suche in Google Scholar
26. CannetC. Artéfacts: les difficultés de la technique histologique de la circulation à la coupe. Rev Fr Histotechnol2006;19:71–84.Suche in Google Scholar
27. BurtisCA, AshwoodER. Tietz text book of clinical biochemistry, 2nd ed., vol. 833. London: Saunders, 1986:1890–1.Suche in Google Scholar
28. KaluDN. The ovariectomized rat model of postmenopausal bone loss. Bone Miner1991;15:175–91.10.1016/0169-6009(91)90124-ISuche in Google Scholar
29. NotomiT, OkimotoN, OkazakiY, NakamuraT, SuzukiM. Tower climbing exercise started 3 months after ovariectomy recovers bone strength of the femur and lumbar vertebrae in aged osteopenic rats. J Bone Miner Res2003;18:140–9.10.1359/jbmr.2003.18.1.140Suche in Google Scholar PubMed
30. Zhen-GuoL, RongZ, ChenL, XueM, LiL, Jie-PinW, et al. The osteoprotective effect of Radix Dipsaci extract in ovariectomized rats. J Ethnopharmacol2009;123:74–8.10.1016/j.jep.2009.02.025Suche in Google Scholar PubMed
31. BashaME, ChangS, BurrowsLJ, LassmannJ, WeinAJ, MorelandRS, et al. Effect of estrogen on molecular and functional characteristics of the rodent vaginal muscularis. J Sex Med2013. Epub ahead of print. DOI:10.1111/jsm.12088.Suche in Google Scholar
32. DielP, GeisRB, CaldarelliA, SchmidtS, LeschowskyUL,VossA, et al. The differential ability of the phytoestrogen genistein and of estradiol to induce uterine weight and proliferation in the rat is associated with a substance specific modulation of uterine gene expression. Mol Cell Endocrinol2004;221:21–32.10.1016/j.mce.2004.04.006Suche in Google Scholar
33. MvondoMA, NjamenD, Tanee FomumS, WandjiJ. Effects of alpinumisoflavone and abyssinone V-4'-methyl ether derived from Erythrina lysistemon (Fabaceae) on the genital tract of ovariectomized female Wistar rat. Phytother Res2012;26:1029–36.10.1002/ptr.3685Suche in Google Scholar
34. NjamenD, DjiogueS, ZingueS, MvondoMA, Nkeh-ChungagBN. In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae). J Complement Integr Med2013;10:1–11.10.1515/jcim-2013-0018Suche in Google Scholar
35. NjamenD, Nkeh-ChungagBN, MvondoMA, TchoukouegnoNS. Oestrogenic properties of the ethanolic extract of Fernandoa adolfi friderici (Bignoniaceae) stem bark. Afr J Pharm Pharmacol2013;7:1729–36.10.5897/AJPP12.1308Suche in Google Scholar
36. HeaneyRP, ReckerRR, WatsonP, LappeJM. Phosphate and carbonate salts of calcium support robust bone building in osteoporosis. Am J Clin Nutr2010;92:101–5.10.3945/ajcn.2009.29085Suche in Google Scholar
37. SwaminathanR. Biochemical markers of bone turnover. Clin Chim Acta2001;313:95–105.10.1016/S0009-8981(01)00656-8Suche in Google Scholar
38. YongzhongZ, LongjiangY, MingzhangA, WenwenJ. Effect of ethanol extract of Lepidium meyenii Walp on osteoporosis in ovariectomized rat. J Ethnopharmacol2006;105:274–9.10.1016/j.jep.2005.12.013Suche in Google Scholar
39. CompstonJ. Bone histomorphometry: the renaissance?BoneKey-Osteovision2004;5:9–12.10.1138/20040129Suche in Google Scholar
40. BonjourJP, AmmanP, RizzoliR. Importance of preclinical studies in the development of drugs for treatment of osteoporosis: a review related to the 1998 WHO guidelines. Osteoporos Int1999;9:379–93.10.1007/s001980050161Suche in Google Scholar
41. SilvestiniG, BallantiP, PatacchiioliFR, MocettiP, Di GreziaR, WedardBM, et al. Evaluation of apoptosis and the glucocorticoid receptor in the cartilage growth plate and metaphyseal bone cells of rats after high dose treatment with corticosterone. Bone2000;26:33–42.10.1016/S8756-3282(99)00245-8Suche in Google Scholar
42. MageeJP, RowlandIR. Phytoestrogen, their mechanismof action current evidence for o role in breast and prostate cancer. Br J Nutr2004;91:513–31.10.1079/BJN20031075Suche in Google Scholar PubMed
©2014 by Walter de Gruyter Berlin / Boston
Artikel in diesem Heft
- Frontmatter
- Review
- A review on Ipomoea carnea: pharmacology, toxicology and phytochemistry
- Preclinical Studies
- Biochemical and histopathological study of the anti-hyperglycemic and anti-hyperlipidemic effects of cornelian cherry (Cornus mas L.) in alloxan-induced diabetic rats
- Pharmacognostical standardization and antidiabetic activity of Syzygium cumini (Linn.) barks (Myrtaceae) on streptozotocin-induced diabetic rats
- Preventive effects of the methanol soluble fraction of Millettia macrophylla Benth (Fabaceae) on an osteoporosis-like model of ovariectomized Wistar rats
- Detoxification of Echis ocellatus venom-induced toxicity by Annona senegalensis Pers
- Subacute antidiabetic and in vivo antioxidant effects of methanolic extract of Bridelia micrantha (Hochst Baill) leaf on alloxan-induced hyperglycaemic rats
- Effect of Persea americana (avocado) fruit extract on the level of expression of adiponectin and PPAR-γ in rats subjected to experimental hyperlipidemia and obesity
- In vitro protective effects of Scutellaria litwinowii root extract against H2O2-induced DNA damage and cytotoxicity
- Clinical Studies
- The antiplaque effect of herbal mouthwash in comparison to chlorhexidine in human gingival disease: a randomized placebo controlled clinical trial
- Use of complementary and alternative medical therapies (CAM) by patients attending a regional comprehensive cancer care centre
- Education Forum
- Implementing blended learning into the academic programs of Rajiv Gandhi University of Health Sciences, Karnataka
Artikel in diesem Heft
- Frontmatter
- Review
- A review on Ipomoea carnea: pharmacology, toxicology and phytochemistry
- Preclinical Studies
- Biochemical and histopathological study of the anti-hyperglycemic and anti-hyperlipidemic effects of cornelian cherry (Cornus mas L.) in alloxan-induced diabetic rats
- Pharmacognostical standardization and antidiabetic activity of Syzygium cumini (Linn.) barks (Myrtaceae) on streptozotocin-induced diabetic rats
- Preventive effects of the methanol soluble fraction of Millettia macrophylla Benth (Fabaceae) on an osteoporosis-like model of ovariectomized Wistar rats
- Detoxification of Echis ocellatus venom-induced toxicity by Annona senegalensis Pers
- Subacute antidiabetic and in vivo antioxidant effects of methanolic extract of Bridelia micrantha (Hochst Baill) leaf on alloxan-induced hyperglycaemic rats
- Effect of Persea americana (avocado) fruit extract on the level of expression of adiponectin and PPAR-γ in rats subjected to experimental hyperlipidemia and obesity
- In vitro protective effects of Scutellaria litwinowii root extract against H2O2-induced DNA damage and cytotoxicity
- Clinical Studies
- The antiplaque effect of herbal mouthwash in comparison to chlorhexidine in human gingival disease: a randomized placebo controlled clinical trial
- Use of complementary and alternative medical therapies (CAM) by patients attending a regional comprehensive cancer care centre
- Education Forum
- Implementing blended learning into the academic programs of Rajiv Gandhi University of Health Sciences, Karnataka
