Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide
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Gabriel O. Oludare
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Gbenga O. Afolayan
Abstract
Objectives
This study aimed to access the protective effects of d-ribose-l-cysteine (DRLC) on cyclophosphamide (CPA) induced gonadal toxicity in male rats.
Methods
Forty-eight male Sprague-Dawley rats were divided into six groups of eight rats each. Group I the control, received distilled water (10 ml/kg), Group II received a single dose of CPA 100 mg/kg body weight intraperitoneally (i.p), Groups III and IV received a single dose of CPA at 100 mg/kg (i.p) and then were treated with DRLC at 200 mg/kg bodyweight (b.w) and 400 mg/kg b.w for 10 days, respectively. Rats in Groups V and VI received DRLC at 200 and 400 mg/kg b.w for 10 days, respectively. DRLC was administered orally.
Results
Results showed that CPA increased percentage of abnormal sperm cells and reduced body weight, sperm count, sperm motility, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels (p<0.05). CPA also induced oxidative stress as indicated by the increased malondialdehyde (MDA) content and reduced activities of the oxidative enzymes measured (p<0.05). Liver enzymes were elevated while the blood cells production was decreased in the rats administered CPA. DRLC supplementation enhanced the antioxidant defence system as indicated in the reduced MDA levels and increased activities of the antioxidant enzymes when compared with CPA (p<0.05). Bodyweight, sperm count, sperm motility, FSH, and testosterone levels were increased in the CPA + DRLC II group compared with CPA (p<0.05).
Conclusions
The results of this present study showed that DRLC has a potential protective effect on CPA-induced gonadotoxicity.
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Research funding: None declared.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: Research involving animals complied with all relevant national regulations and institutional policies for the care and use of animals.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Reviews
- N-acetyl-cysteine as adjuvant therapy in female infertility: a systematic review and meta-analysis
- Climate change and declining fertility rate in Malaysia: the possible connexions
- Original Articles
- Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide
- Environmental and climatic impact on the infection and mortality of SARS-CoV-2 in Peru
- Prophylactic intravenous paracetamol use in extremely premature infants for patent ductus arteriosus
- Evaluation of heart rate variability, blood pressure and lipid profile alterations from dual transceiver mobile phone radiation exposure
- Strain specific response of mice to IMQ-induced psoriasis
- Curcumin exerts hepatoprotection via overexpression of Paraoxonase-1 and its regulatory genes in rats undergone bile duct ligation
- Zinc oxide nanoparticles with green tea extract complex in the pancreas of rats against monosodium glutamate toxicity
- Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro
- Protective and curative role of vitamin D and hormones on the cadmium-induced inhibition of proliferation of human osteoblast cells
- The effect of bee pollen on reproductive and biochemical parameters in methotrexate-induced testicular damage in adult rats
- Histomorphometric study on effects of monosodium glutamate in liver tissue of Wistar rats
- Diligent profiling of preclinical safety of the silk protein sericin
Artikel in diesem Heft
- Frontmatter
- Reviews
- N-acetyl-cysteine as adjuvant therapy in female infertility: a systematic review and meta-analysis
- Climate change and declining fertility rate in Malaysia: the possible connexions
- Original Articles
- Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide
- Environmental and climatic impact on the infection and mortality of SARS-CoV-2 in Peru
- Prophylactic intravenous paracetamol use in extremely premature infants for patent ductus arteriosus
- Evaluation of heart rate variability, blood pressure and lipid profile alterations from dual transceiver mobile phone radiation exposure
- Strain specific response of mice to IMQ-induced psoriasis
- Curcumin exerts hepatoprotection via overexpression of Paraoxonase-1 and its regulatory genes in rats undergone bile duct ligation
- Zinc oxide nanoparticles with green tea extract complex in the pancreas of rats against monosodium glutamate toxicity
- Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro
- Protective and curative role of vitamin D and hormones on the cadmium-induced inhibition of proliferation of human osteoblast cells
- The effect of bee pollen on reproductive and biochemical parameters in methotrexate-induced testicular damage in adult rats
- Histomorphometric study on effects of monosodium glutamate in liver tissue of Wistar rats
- Diligent profiling of preclinical safety of the silk protein sericin
