Protective and curative role of vitamin D and hormones on the cadmium-induced inhibition of proliferation of human osteoblast cells
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Aylin Dal Ulutas
and
Fezan Mutlu
Abstract
Objectives
Exposure to cadmium (Cd), which causes environmental and industrial pollution, causes toxicity in many tissues and organs, especially bone, lung and kidney. Hormones, growth factors and other stimuli act on bone tissue through osteoblasts. In this study, it was aimed to determine the effects of Cd on hFOB1.19 osteoblast cells and the protective and healing potentials of estrogen, androgen and vitamin D against the inhibitory effect of Cd on the proliferation.
Methods
hFOB1.19 cells were cultivated in our laboratory using Dulbecco’s Modified Eagle’s Medium-F12, HEPES medium, containing 10% fetal bovine serum, 1% penicillin/streptomycin in 34.5 °C 5%CO2 incubator. To determine its protective potentials for the toxicity of CdCl2, it was previously applied 1,25(OH) 2D vitamin, 17β-estradiol, and 5α-androstane for 72 h to cells. To determine their curative potential, osteoblast cells, which were previously exposed to CdCl2 for 72 h, were administered 1,25(OH) 2D vitamin, 17β-estradiol, and 5α-androstane. Following these applications were determined proliferation by XTT analysis and, the amounts of androgen receptor, estrogen receptor, vitamin D receptor, alkaline phosphatase, osteocalcin and osteoprotegerin by ELISA analysis.
Results
Vitamin D has been both preventive and curative effective to increase cell proliferation, which Cd reduces. Interestingly, estrogen had a preventive effect and androgen had a curative effect.
Conclusions
In addition to showing the negative effects of cadmium on the proliferation of osteoblast cells, this study provides an overview of the effects of hormone and vitamin D applications before and after Cd, and these results may serve as a guide for future studies.
Acknowledgment
This study was supported by Eskişehir Osmangazi University Scientific Research Project Committee with Project Support Number 2016-1372.
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Research funding: This study was supported by Eskişehir Osmangazi University Scientific Research Project Committee with Project Support Number 2016-1372.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Ethical approval: This study does not require ethics committee approval.
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- The effect of bee pollen on reproductive and biochemical parameters in methotrexate-induced testicular damage in adult rats
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Articles in the same Issue
- Frontmatter
- Reviews
- N-acetyl-cysteine as adjuvant therapy in female infertility: a systematic review and meta-analysis
- Climate change and declining fertility rate in Malaysia: the possible connexions
- Original Articles
- Potential anti-toxic effect of d-ribose-l-cysteine supplement on the reproductive functions of male rats administered cyclophosphamide
- Environmental and climatic impact on the infection and mortality of SARS-CoV-2 in Peru
- Prophylactic intravenous paracetamol use in extremely premature infants for patent ductus arteriosus
- Evaluation of heart rate variability, blood pressure and lipid profile alterations from dual transceiver mobile phone radiation exposure
- Strain specific response of mice to IMQ-induced psoriasis
- Curcumin exerts hepatoprotection via overexpression of Paraoxonase-1 and its regulatory genes in rats undergone bile duct ligation
- Zinc oxide nanoparticles with green tea extract complex in the pancreas of rats against monosodium glutamate toxicity
- Phenolic constituents and inhibitory effects of the leaf of Rauvolfia vomitoria Afzel on free radicals, cholinergic and monoaminergic enzymes in rat’s brain in vitro
- Protective and curative role of vitamin D and hormones on the cadmium-induced inhibition of proliferation of human osteoblast cells
- The effect of bee pollen on reproductive and biochemical parameters in methotrexate-induced testicular damage in adult rats
- Histomorphometric study on effects of monosodium glutamate in liver tissue of Wistar rats
- Diligent profiling of preclinical safety of the silk protein sericin
