Correlation between biofilm formation and antimicrobial susceptibility pattern toward extended spectrum β-lactamase (ESBL)- and non-ESBL-producing uropathogenic bacteria
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Md. Emam Hasan
Abstract
Background
Urinary tract infections (UTIs) are the most common bacterial infection encountered worldwide and are associated with significant morbidity and mortality.
Methods
The present study was undertaken to investigate the biofilm-forming ability, antibiotic susceptibility patterns and extended spectrum β-lactamase (ESBL) production of seven uropathogenic isolates comprising both Escherichia coli and Klebsiella pneumoniae. The morphological, cultural and biochemical tests for the identification of the isolates, antibiotic susceptibility test, detection of ESBL production, biofilm formation on 96-well microtiter plate and Congo red agar (CRA) media are performed.
Results
The antimicrobial susceptibility profiles obtained in this study showed that the most active drugs gentamicin, amikacin and imipenem (100% sensitivity) were followed by amoxicillin-clavulanic acid (85% sensitivity), co-trimoxazole, ciprofloxacin (57% sensitivity) ceftazidime and kanamycin (50% sensitivity). All the isolates showed resistance to amoxicillin followed by ceftriaxone and cefotaxime (71% resistance), and the scenario gets more complicated because of the production of ESBL by five isolates (three E. coli isolates and two K. pneumoniae). The strains were also able to form biofilm as tested on CRA medium and by microtiter plate assay. The correlation between ESBL, non-ESBL and biofilm-producing E. coli and K. pneumonia was determined along with the multiple drug resistance patterns of E. coli and K. pneumonia.
Conclusions
The findings of the study indicate that the emergence and rapid spread of such multidrug-resistant pathogens are of great concern. Early detection of ESBL-producing pathogen is of paramount clinical importance; therefore, strict infection control practices as well as therapeutic guidance for confirmed infection can be rapidly initiated.
Acknowledgments
This work was supported by the Department of Microbiology, Stamford University Bangladesh, Dhaka-1217, Bangladesh as part of microbiological research.
Research funding: None declared.
Author contributions: This work was carried out in collaboration between all authors. All the authors have accepted responsibility for the entire content of this submitted manuscript and approved the submission. Authors MEH, AS, and FS performed experiments. TBE, FS, and AKN conceived the study and designed the experimental procedures. MEH, AS, and TBE designed and planned the study and supervised the experiments. TBE and FS also acted for all correspondences. MEH, AS and TBE participated in the manuscript draft and have thoroughly checked and revised the manuscript for necessary changes in format, grammar and English standard. All authors read and approved the final version of the manuscript. All authors read and approved the manuscript.
Competing interests: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
References
[1] Howard SJ, Hopwood S, Davies SC. Antimicrobial resistance: a global challenge. Sci Transl Med 2014;6:236ed210.10.1126/scitranslmed.3009315Suche in Google Scholar PubMed
[2] Girish N, Saileela K, Mohanty SK. Extended spectrum β-lactamase producing Klebsiella pneumoniae and Escherichia coli in neonatal intensive care unit. J Bacteriol Parasitol 2012;3:1–3.10.4172/2155-9597.1000141Suche in Google Scholar
[3] Foxman B. Epidemiology of urinary tract infections: incidence, morbidity, and economic costs. Disease-a-month. Dis Mon 2003;49:53–70.10.1067/mda.2003.7Suche in Google Scholar PubMed
[4] Flores-mireles AL, Walker JN, Caparon M, Hultgren SJ. Urinary tract infections: epidemiology, mechanisms of infection and treatment options. Nature Rev Microbiol 2015;13:269–84.10.1038/nrmicro3432Suche in Google Scholar PubMed PubMed Central
[5] Paterson DL. Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Med 2006;34:S20–8; discussion S64–73.10.1016/j.amjmed.2006.03.013Suche in Google Scholar PubMed
[6] Hancock V, Ferrières L, Klemm P. The ferric yersiniabactin uptake receptor FyuA is required for efficient biofilm formation by urinary tract infectious Escherichia coli in human urine. Microbiology 2008;154:167–75.10.1099/mic.0.2007/011981-0Suche in Google Scholar PubMed
[7] Wu Y, Outten FW. IscR controls iron-dependent biofilm formation in Escherichia coli by regulating type I fimbria expression. J Bacteriol 2009;191:1248–57.10.1128/JB.01086-08Suche in Google Scholar PubMed PubMed Central
[8] Cappuccino JG, Sherman N. Microbiology: a laboratory manual. Menlo Park, CA: The Benjamin/Cummings Publishing Co., 2005.Suche in Google Scholar
[9] CLSI. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. Twenty-First Informational Supplement. CLSI document M100-S21. Wayne, PA: Clinical and Laboratory Standards Institute, 2011.Suche in Google Scholar
[10] CLSI. Performance standards for antimicrobial susceptibility testing. Twenty-Fourth Informational Supplement. CLSI document M100-S24. Wayne, PA: Clinical and Laboratory Standards Institute, 2014.Suche in Google Scholar
[11] Khan SA, Feroz F, Noor R. Study of extended-spectrum β-lactamase-producing bacteria from urinary tract infections in Bangladesh. Tzu Chi Medical J 2013;25:39–42.10.1016/j.tcmj.2013.01.008Suche in Google Scholar
[12] Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 1989;42:872–4.10.1136/jcp.42.8.872Suche in Google Scholar PubMed PubMed Central
[13] Pierce CG, Uppuluri P, Tummala, Lopez-Ribot JL. A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. J Vis Exp 2010;44:1–4.10.3791/2287Suche in Google Scholar PubMed PubMed Central
[14] Bagshaw SM, Laupland KB. Epidemiology of intensive care unit-acquired urinary tract infections. Curr Opin Infect Dis 2006;19:67–71.10.1097/01.qco.0000200292.37909.e0Suche in Google Scholar
[15] Pitout JD, Sanders CC, Sanders WE. Antimicrobial resistance with focus on beta-lactam resistance in gram-negative bacilli. Am J Med Sci 1997;103:51–9.10.1016/S0002-9343(97)00044-2Suche in Google Scholar
[16] Ponnusamy P, Natarajan V, Sevanan M. In vitro biofilm formation by uropathogenic Escherichia coli and their antimicrobial susceptibility pattern. Asian Pac J Trop Biomed 2012;5:210–3.10.1016/S1995-7645(12)60026-1Suche in Google Scholar
[17] Marhova M, Kostadinova S, Stoitsova S. Antimicrobial resistance profiles of urinary Escherichia coli isolates. Biotechnol Biotechnol Equip 2009;23:616–20.10.1080/13102818.2009.10818500Suche in Google Scholar
[18] Parajuli NP, Maharjan P, Parajuli H, Joshi G, Paudel D, Sayami S, Khanal PR. High rates of multidrug resistance among uropathogenic Escherichia coli in children and analyses of ESBL producers from Nepal. Antimicrob Resist Infect Control 2017;6:9.10.1186/s13756-016-0168-6Suche in Google Scholar PubMed PubMed Central
[19] Akpaka PE, Swanston WH. Phenotypic detection and occurrence of extended-spectrum beta-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli at a tertiary hospital in Trinidad & Tobago. Braz J Infect Dis 2008;12:516–20.10.1590/S1413-86702008000600014Suche in Google Scholar PubMed
[20] Colodner R, Raz R, Chazan B, Sakran W. Susceptibility pattern of extended-spectrum beta-lactamase producing bacteria isolated from inpatients to five antimicrobial drugs in a community hospital in Northern Israel. Int J Antimicrob Agents 2004;24:409–10.10.1016/j.ijantimicag.2004.06.001Suche in Google Scholar PubMed
[21] Pfaller MA, Davenport D, Bale M, Koontz F, Massanari R. Development of the quantitative micro-test for slime production by coagulase-negative Staphylococci. Eur J Clin Microbiol Infect Dis 1988;7:30–3.10.1007/BF01962167Suche in Google Scholar PubMed
[22] Sharma M, Yadav S, Chaudhary U. Biofilm production in uropathogenic Escherichia coli. Indian J Pathol Microbiol 2009;52:294.10.4103/0377-4929.48960Suche in Google Scholar PubMed
[23] Poovendran P, Vidhya N, Murugan S. Antimicrobial susceptibility pattern of ESBL and non-ESBL producing uropathogenic Escherichia coli (UPEC) and their correlation with biofilm formation. Microbiol Res J Int 2013;4:56–63.Suche in Google Scholar
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Artikel in diesem Heft
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Artikel in diesem Heft
- Frontmatter
- Review
- Update of hypothyroidism and its management in Unani medicine
- Original Articles
- 6-OHDA mediated neurotoxicity in SH-SY5Y cellular model of Parkinson disease suppressed by pretreatment with hesperidin through activating L-type calcium channels
- Modulation of sleep using electrical vestibular nerve stimulation prior to sleep onset: a pilot study
- Nitric oxide mediates the antidepressant-like effect of modafinil in mouse forced swimming and tail suspension tests
- Effect of mobile phone usage on cognitive functions, sleep pattern, visuospatial ability in Parkinsons patients; a possible correlation with onset of clinical symptoms
- Food-added azodicarbonamide alters haematogical parameters, antioxidant status and biochemical/histomorphological indices of liver and kidney injury in rats
- Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes
- p53 upregulated mediator of apoptosis (Puma) deficiency increases survival of adult neural stem cells generated physiologically in the hippocampus, but does not protect stem cells generated in surplus after an excitotoxic lesion
- In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats
- Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats
- Amine-modified kaolinite clay preserved thyroid function and renal oxidative balance after sub-acute exposure in rats
- Aqueous extract of dry powder blend of seeds and leaves of Picralima nitida (Stapf) T. & H. Durand reduce pain and inflammation in animal models
- Methanol extract of Caesalpinia benthamiana normalizes blood pressure and attenuates oxidative stress in uninephrectomized hypertensive rats
- Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice
- Correlation between biofilm formation and antimicrobial susceptibility pattern toward extended spectrum β-lactamase (ESBL)- and non-ESBL-producing uropathogenic bacteria
