6-OHDA mediated neurotoxicity in SH-SY5Y cellular model of Parkinson disease suppressed by pretreatment with hesperidin through activating L-type calcium channels
-
Leila Elyasi
,
Mehrdad Jahanshahi
Abstract
Objectives
Parkinson’s disease (PD) is a neurological condition with selective progressive degeneration of dopaminergic neurons. Routine therapies are symptomatic and palliative. Although, hesperidin (Hsd) is known for its neuroprotective effects, its exact cellular mechanism is still a mystery. Considering the important role of calcium (Ca2+) in cellular mechanisms of neurodegenerative diseases, the present study aimed to investigate the possible effects of Hsd on Ca2+ channels in cellular model of PD and the possible association between the selective vulnerability of neurons in cellular models of PD and expression of the physiological phenotype that changes Ca2+ homeostasis.
Methods
SH-SY5Y cell line was used in this study; cell damage was induced by 150 µM 6-OHDA and the cells’ viability was examined using MTT assay. Intracellular calcium, reactive oxygen species (ROS) and mitochondrial membrane potential were determined by the fluorescence spectrophotometry method. The expressions of calcium channel receptors were determined by gel electrophoresis and immunoblotting.
Results
Loss of cell viability and mitochondrial membrane potential were confirmed in 6-OHDA treated cells. In addition, intracellular ROS and calcium levels, calcium channel receptors significantly increased in 6-OHDA-treated cells. Incubation of SH-SY5Y cells with hesperidin showed a protective effect, reduced the biochemical markers of cell damage/death, and balanced calcium hemostasis.
Conclusions
Based on our findings, it seems that hesperidin could suppress the progression of the cellular model of PD via acting on intracellular calcium homeostasis. Further studies are needed to confirm the potential benefits of preventive and therapeutic effects of stabilizing cellular calcium homeostasis in neurodegenerative disease.
Funding source: Golestan University of Medical Sciences (Gorgan, Iran)
Award Identifier / Grant number: 96122308
Acknowledgments
We would like to express our gratitude to the Neuroscience Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Research funding: This work was supported by the Deputy of Research and Technology, Golestan University of Medical Sciences (Gorgan, Iran) under Grant number 96122308. In addition, all procedures were in accordance with the ethical principles accepted in the Golestan University of Medical Sciences. (IR. goums.REC.1396314).
Author contributions: Study concept and design: L. Elyasi; M .Jahanshahi data acquisition: L. Elyasi; H.Ghasemi; data analysis and interpretation: L. Elyasi; khalili Masoumeh drafting of the manuscript: L. Elyasi; E.G. Nikmahzar and study supervision: L. Elyasi. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Informed consent: Informed consent was obtained from all individuals included in this study.
Ethical approval: This work was approved by Ethical committee of the Deputy of Research and Technology, Golestan University of Medical Sciences (Gorgan, Iran).
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Review
- Update of hypothyroidism and its management in Unani medicine
- Original Articles
- 6-OHDA mediated neurotoxicity in SH-SY5Y cellular model of Parkinson disease suppressed by pretreatment with hesperidin through activating L-type calcium channels
- Modulation of sleep using electrical vestibular nerve stimulation prior to sleep onset: a pilot study
- Nitric oxide mediates the antidepressant-like effect of modafinil in mouse forced swimming and tail suspension tests
- Effect of mobile phone usage on cognitive functions, sleep pattern, visuospatial ability in Parkinsons patients; a possible correlation with onset of clinical symptoms
- Food-added azodicarbonamide alters haematogical parameters, antioxidant status and biochemical/histomorphological indices of liver and kidney injury in rats
- Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes
- p53 upregulated mediator of apoptosis (Puma) deficiency increases survival of adult neural stem cells generated physiologically in the hippocampus, but does not protect stem cells generated in surplus after an excitotoxic lesion
- In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats
- Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats
- Amine-modified kaolinite clay preserved thyroid function and renal oxidative balance after sub-acute exposure in rats
- Aqueous extract of dry powder blend of seeds and leaves of Picralima nitida (Stapf) T. & H. Durand reduce pain and inflammation in animal models
- Methanol extract of Caesalpinia benthamiana normalizes blood pressure and attenuates oxidative stress in uninephrectomized hypertensive rats
- Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice
- Correlation between biofilm formation and antimicrobial susceptibility pattern toward extended spectrum β-lactamase (ESBL)- and non-ESBL-producing uropathogenic bacteria
Articles in the same Issue
- Frontmatter
- Review
- Update of hypothyroidism and its management in Unani medicine
- Original Articles
- 6-OHDA mediated neurotoxicity in SH-SY5Y cellular model of Parkinson disease suppressed by pretreatment with hesperidin through activating L-type calcium channels
- Modulation of sleep using electrical vestibular nerve stimulation prior to sleep onset: a pilot study
- Nitric oxide mediates the antidepressant-like effect of modafinil in mouse forced swimming and tail suspension tests
- Effect of mobile phone usage on cognitive functions, sleep pattern, visuospatial ability in Parkinsons patients; a possible correlation with onset of clinical symptoms
- Food-added azodicarbonamide alters haematogical parameters, antioxidant status and biochemical/histomorphological indices of liver and kidney injury in rats
- Conditioned medium from the human umbilical cord-mesenchymal stem cells stimulate the proliferation of human keratinocytes
- p53 upregulated mediator of apoptosis (Puma) deficiency increases survival of adult neural stem cells generated physiologically in the hippocampus, but does not protect stem cells generated in surplus after an excitotoxic lesion
- In vitro antioxidants and hepatoprotective effects of Pleurotus tuber-regium on carbon tetrachloride–treated rats
- Mitigative effect of Momordica cymbalaria fruit extract against sodium fluoride induced hepatotoxicity in Wistar male albino rats
- Amine-modified kaolinite clay preserved thyroid function and renal oxidative balance after sub-acute exposure in rats
- Aqueous extract of dry powder blend of seeds and leaves of Picralima nitida (Stapf) T. & H. Durand reduce pain and inflammation in animal models
- Methanol extract of Caesalpinia benthamiana normalizes blood pressure and attenuates oxidative stress in uninephrectomized hypertensive rats
- Evaluation of antidiabetic effect of Cistus salviifolius L. (Cistaceae) in streptozotocin-nicotinamide induced diabetic mice
- Correlation between biofilm formation and antimicrobial susceptibility pattern toward extended spectrum β-lactamase (ESBL)- and non-ESBL-producing uropathogenic bacteria
