Abstract
Aqueous enzymatic extraction (AEE) is a new technology for extracting vegetable oil body which has the advantages of low energy consumption, product safety, mild reaction conditions, and simultaneous separation of oil and protein. Among the enzymes tested in the present work, Viscozyme L (compound plant hydrolase) exhibited the highest extraction activity during peanut oil extraction. Extraction was optimized using response surface methodology, and optimal conditions were enzymatic temperature 51.5 °C, material-to-liquid ratio 1:3.5, enzymatic concentration 1.5%, and enzymatic time 90 min, yielding total oil body and protein of 93.67 ± 0.59% and 76.84 ± 0.68%, respectively. The fatty acid composition and content, and various quality indicators were not significantly different from those of cold-pressed oil, hence peanut oil produced by AEE met the same standards as cold-pressed first-grade peanut oil. Additionally, the functional properties of peanut protein produced by AEE were superior to those of commercially available peanut protein.
Award Identifier / Grant number: 2018YFD0401100
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 21676073
-
Author contributions: Y. Gao and C. Liu designed and conducted the experiments, performed data analysis, and wrote the manuscript. F. Chen supervised the study and helped to initiate the project. R. Niu revised the manuscript. F. Yao, Y. Song and M. Liu helped complete the experiment.
-
Research funding: This study was financially supported by Domain Foundation of Equipment Advance Research of 13th Five-year Plan (2018YFD0401100) and the National Natural Science Foundation of China (21676073).
-
Conflict of interest statement: We declare that we have no conflict of interest.
References
1. Dong, XH, Zhao, M, Shi, J, Yang, B, Li, J, Luo, D, et al.. Effects of combined high-pressure homogenization and enzymatic treatment on extraction yield, hydrolysis and function properties of peanut proteins. Innovat Food Sci Emerg Technol 2011;12:478–83. https://doi.org/10.1016/j.ifset.2011.07.002.Suche in Google Scholar
2. Yu, J, Ahmedna, M, Goktepe, I, Cheng, H, Maleki, S. Enzymatic treatment of peanut kernels to reduce allergen levels. Food Chem 2011;127:1014–22. https://doi.org/10.1016/j.foodchem.2011.01.074.Suche in Google Scholar PubMed
3. Basse, B, Bosc, V, Saiter, J-M, Chan-Huot, M, Dupas, J-P, Maillard, M-N, et al.. Combined effects of ionic strength and enzymatic pre-treatment in thermal gelation of peanut proteins extracts. Food Res Int 2020;137:109362. https://doi.org/10.1016/j.foodres.2020.109362.Suche in Google Scholar PubMed
4. Dun, Q, Yao, L, Deng, Z, Li, H, Li, J, Fang, Y, et al.. Effects of hot and cold-pressed processes on volatile compounds of peanut oil and corresponding analysis of characteristic flavor components. LWT - Food Sci Technol 2019;112:107648. https://doi.org/10.1016/j.lwt.2018.11.084.Suche in Google Scholar
5. Akram, NA, Shafiq, F, Ashraf, M. Peanut (Arachis hypogaea l.): a prospective legume crop to offer multiple health benefits under changing climate. Compr Rev Food Sci Food Saf 2018;17:1325–38. https://doi.org/10.1111/1541-4337.12383.Suche in Google Scholar PubMed
6. Suri, K, Singh, B, Kaur, A, Singh, N. Impact of roasting and extraction methods on chemical properties, oxidative stability and Maillard reaction products of peanut oils. J Food Sci Technol-Mysore 2019;56:2436–45. https://doi.org/10.1007/s13197-019-03719-4.Suche in Google Scholar PubMed PubMed Central
7. Jiang, L, Hua, D, Wang, Z, Xu, S. Aqueous enzymatic extraction of peanut oil and protein hydrolysates. Food Bioprod Process 2010;88:233–8. https://doi.org/10.1016/j.fbp.2009.08.002.Suche in Google Scholar
8. Ma, T, Wang, Q, Wu, H. Optimization of extraction conditions for improving solubility of peanut protein concentrates by response surface methodology. LWT - Food Sci Technol 2010;43:1450–5. https://doi.org/10.1016/j.lwt.2010.03.015.Suche in Google Scholar
9. He, X-H, Liu, H-Z, Liu, L, Zhao, G-L, Wang, Q, Chen, Q-L. Effects of high pressure on the physicochemical and functional properties of peanut protein isolates. Food Hydrocolloids 2014;36:123–9. https://doi.org/10.1016/j.foodhyd.2013.08.031.Suche in Google Scholar
10. Amigh, S, Dinani, ST. Combination of ultrasound-assisted aqueous enzymatic extraction and cooking pretreatment for date seed oil recovery. Heat Mass Tran 2020;56:2345–54. https://doi.org/10.1007/s00231-020-02865-2.Suche in Google Scholar
11. Zheng, L, Ren, J, Su, G, Yang, B, Zhao, M. Comparison of in vitro digestion characteristics and antioxidant activity of hot- and cold-pressed peanut meals. Food Chem 2013;141:4246–52. https://doi.org/10.1016/j.foodchem.2013.06.081.Suche in Google Scholar PubMed
12. Liu, J-J, Gasmalla, MAA, Li, P, Yang, R. Enzyme-assisted extraction processing from oilseeds: principle, processing and application. Innovat Food Sci Emerg Technol 2016;35:184–93. https://doi.org/10.1016/j.ifset.2016.05.002.Suche in Google Scholar
13. Geng, Q, Chen, J, Guo, R, Zhang, L, Li, Q, Yu, X. Salt-assisted aqueous extraction combined with span 20 allow the obtaining of a high-quality and yield walnut oil. LWT - Food Sci Technol 2020;121:108956. https://doi.org/10.1016/j.lwt.2019.108956.Suche in Google Scholar
14. Zhou, L-Z, Chen, F-S, Hao, L-H, Du, Y, Liu, C. Peanut oil body composition and stability. J Food Sci 2019;84:2812–9. https://doi.org/10.1111/1750-3841.14801.Suche in Google Scholar PubMed
15. Zhang, SB, Lu, QY. Characterizing the structural and surface properties of proteins isolated before and after enzymatic demulsification of the aqueous extract emulsion of peanut seeds. Food Hydrocolloids 2015;47:51–60. https://doi.org/10.1016/j.foodhyd.2015.01.007.Suche in Google Scholar
16. Qian, J-Q, Qin, D-H, Xie, X-M, Zhou, W-W. Study on enzyme-assisted aqueous extraction of oil from soybean. J Sci Ind Res 2010;69:860–5.Suche in Google Scholar
17. Niu, Y, Li, W, Zhu, J, Huang, Q, Jiang, M, Huang, F, et al.. Aqueous enzymatic extraction of rapeseed oil and protein from dehulled cold-pressed double-low rapeseed cake. Int J Food Eng 2012;8. https://doi.org/10.1515/1556-3758.2530.Suche in Google Scholar
18. de Aquino, DS, Fanhani, A, Stevanato, N, da Silva, C. Sunflower oil from enzymatic aqueous extraction process: maximization of free oil yield and oil characterization. J Food Process Eng 2019;42:10. https://doi.org/10.1111/jfpe.13169.Suche in Google Scholar
19. Zhang, WC, Leong, SM, Zhao, F, Zhao, F, Yang, T, Liu, S. Viscozyme l pretreatment on palm kernels improved the aroma of palm kernel oil after kernel roasting. Food Res Int 2018;107:172–81. https://doi.org/10.1016/j.foodres.2018.02.023.Suche in Google Scholar PubMed
20. Nguyen, HC, Vuong, DP, Nguyen, NTT, Nguyen, NP, Su, CH, Wang, FM, et al.. Aqueous enzymatic extraction of polyunsaturated fatty acid-rich sacha inchi (Plukenetia volubilis l.) seed oil: an eco-friendly approach. LWT - Food Sci Technol 2020;133:109992. https://doi.org/10.1016/j.lwt.2020.109992.Suche in Google Scholar
21. Niu, R-H, Chen, F-S, Zhao, Z-T, Xin, Y, Duan, X-J, Wang, B-Y. Effect of papain on the demulsification of peanut oil body emulsion and the corresponding mechanism. J Oleo Sci 2020;69:617–25. https://doi.org/10.5650/jos.ess19297.Suche in Google Scholar PubMed
22. Chen, F, Zhang, Q, Gu, H, Yang, L. An approach for extraction of kernel oil from Pinus pumila using homogenate-circulating ultrasound in combination with an aqueous enzymatic process and evaluation of its antioxidant activity. J Chromatogr A 2016;1471:68–79. https://doi.org/10.1016/j.chroma.2016.10.037.Suche in Google Scholar
23. Liu, Z, Gui, M, Xu, T, Zhang, L, Kong, L, Qin, L, et al.. Efficient aqueous enzymatic-ultrasonication extraction of oil from Sapindus mukorossi seed kernels. Ind Crop Prod 2019;134:124–33. https://doi.org/10.1016/j.indcrop.2019.03.065.Suche in Google Scholar
24. Zheng, L, Zhao, Y, Xiao, C, Sun-Waterhouse, D, Zhao, M, Su, G. Mechanism of the discrepancy in the enzymatic hydrolysis efficiency between defatted peanut flour and peanut protein isolate by flavorzyme. Food Chem 2015;168:100–6. https://doi.org/10.1016/j.foodchem.2014.07.037.Suche in Google Scholar
25. Yoshida, BY, Prudencio, SH. Physical, chemical, and technofunctional properties of okara modified by a carbohydrase mixture. LWT 2020;134:110141. https://doi.org/10.1016/j.lwt.2020.110141.Suche in Google Scholar
26. Lowry, OH, Rosebrough, N, Farr, AL, Randall, R. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265–75. https://doi.org/10.1016/s0021-9258(19)52451-6.Suche in Google Scholar
27. Yu, L, Yang, W, Sun, J, Zhang, C, Bi, J, Yang, Q. Preparation, characterisation and physicochemical properties of the phosphate modified peanut protein obtained from arachin conarachin l. Food Chem 2015;170:169–79. https://doi.org/10.1016/j.foodchem.2014.08.047.Suche in Google Scholar PubMed
28. Cumby, N, Zhong, Y, Naczk, M, Shahidi, F. Antioxidant activity and water-holding capacity of canola protein hydrolysates. Food Chem 2008;109:144–8. https://doi.org/10.1016/j.foodchem.2007.12.039.Suche in Google Scholar PubMed
29. Wu, HW, Wang, Q, Ma, T, Ren, J. Comparative studies on the functional properties of various protein concentrate preparations of peanut protein. Food Res Int 2009;42:343–8. https://doi.org/10.1016/j.foodres.2008.12.006.Suche in Google Scholar
30. Jung, S, Maurer, D, Johnson, LA. Factors affecting emulsion stability and quality of oil recovered from enzyme-assisted aqueous extraction of soybeans. Bioresour Technol 2009;100:5340–7. https://doi.org/10.1016/j.biortech.2009.03.087.Suche in Google Scholar PubMed
31. Yusoff, MM, Gordon, MH, Ezeh, O, Niranjan, K. Aqueous enzymatic extraction of moringa oleifera oil. Food Chem 2016;211:400–8. https://doi.org/10.1016/j.foodchem.2016.05.050.Suche in Google Scholar PubMed
32. Latif, S, Anwar, F. Effect of aqueous enzymatic processes on sunflower oil quality. J Am Oil Chem Soc 2009;86:393–400. https://doi.org/10.1007/s11746-009-1357-8.Suche in Google Scholar
33. Diaz-Suarez, P, Rosales-Quintero, A, Fernandez-Lafuente, R, Pola-Sanchez, E, Hernandez-Cruz, MC, Ovando-Chacon, SL, et al.. Aqueous enzymatic extraction of Ricinus communis seeds oil using viscozyme l. Ind Crop Prod 2021;170:113811. https://doi.org/10.1016/j.indcrop.2021.113811.Suche in Google Scholar
34. Su, CH, Pham, TTT, Cheng, HH. Aqueous enzymatic extraction of rosmarinic acid from Salvia officinalis: optimisation using response surface methodology. Phytochem Anal 2020;31:575–82. https://doi.org/10.1002/pca.2922.Suche in Google Scholar PubMed
35. Zhang, SB, Wang, Z, Xu, SY. Optimization of the aqueous enzymatic extraction of rapeseed oil and protein hydrolysates. J Am Oil Chem Soc 2007;84:97–105. https://doi.org/10.1007/s11746-006-1004-6.Suche in Google Scholar
36. Wang, J, Sun, B, Liu, Y, Zhang, H. Optimisation of ultrasound-assisted enzymatic extraction of arabinoxylan from wheat bran. Food Chem 2014;150:482–8. https://doi.org/10.1016/j.foodchem.2013.10.121.Suche in Google Scholar PubMed
37. Kumar, SPJ, Prasad, SR, Banerjee, R, Agarwal, DK, Kulkarni, KS, Ramesh, KV. Green solvents and technologies for oil extraction from oilseeds. Chem Cent J 2017;11:7. https://doi.org/10.1186/s13065-017-0238-8.Suche in Google Scholar PubMed PubMed Central
38. Tian, Y, Xu, Z, Zheng, B, Martin Lo, Y. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum l.) seed oil. Ultrason Sonochem 2013;20:202–8. https://doi.org/10.1016/j.ultsonch.2012.07.010.Suche in Google Scholar PubMed
39. Su, C-H, Nguyen, HC, Bui, TL, Huang, D-L. Enzyme-assisted extraction of insect fat for biodiesel production. J Clean Prod 2019;223:436–44. https://doi.org/10.1016/j.jclepro.2019.03.150.Suche in Google Scholar
40. Boulila, A, Hassen, I, Haouari, L, Mejri, F, Amor, IB, Casabianca, H, et al.. Enzyme-assisted extraction of bioactive compounds from bay leaves (Laurus nobilis l.). Ind Crop Prod 2015;74:485–93. https://doi.org/10.1016/j.indcrop.2015.05.050.Suche in Google Scholar
41. Chen, J, Yu, X, Geng, Q, Li, M. Combination of span 20 and ph-assisted walnut oil extraction during aqueous extraction process. LWT - Food Sci Technol 2018;91:477–83. https://doi.org/10.1016/j.lwt.2018.01.091.Suche in Google Scholar
42. Yusoff, MM, Gordon, MH, Niranjan, K. Aqueous enzyme assisted oil extraction from oilseeds and emulsion de-emulsifying methods: a review. Trends Food Sci Technol 2015;41:60–82. https://doi.org/10.1016/j.tifs.2014.09.003.Suche in Google Scholar
43. Hu, B, Li, Y, Song, J, Li, H, Zhou, Q, Li, C, et al.. Oil extraction from tiger nut (Cyperus esculentus l.) using the combination of microwave-ultrasonic assisted aqueous enzymatic method - design, optimization and quality evaluation. J Chromatogr A 2020;1627:461380. https://doi.org/10.1016/j.chroma.2020.461380.Suche in Google Scholar PubMed
44. Hu, H, Liu, HZ, Shi, AM, Liu, L, Fauconnier, ML, Wang, Q. The effect of microwave pretreatment on micronutrient contents, oxidative stability and flavor quality of peanut oil. Molecules 2019;24:12. https://doi.org/10.3390/molecules24010062.Suche in Google Scholar PubMed PubMed Central
45. Toomer, OT. Nutritional chemistry of the peanut (Arachis hypogaea). Crit Rev Food Sci Nutr 2018;58:3042–53. https://doi.org/10.1080/10408398.2017.1339015.Suche in Google Scholar PubMed
46. Isleib, TG, Pattee, HE, Sanders, TH, Hendrix, KW, Dean, LO. Compositional and sensory comparisons between normal- and high-oleic peanuts. J Agric Food Chem 2006;54:1759–63. https://doi.org/10.1021/jf052353t.Suche in Google Scholar PubMed
47. Akhtar, S, Khalid, N, Ahmed, I, Shahzad, A, Suleria, HAR. Physicochemical characteristics, functional properties, and nutritional benefits of peanut oil: a review. Crit Rev Food Sci Nutr 2014;54:1562–75. https://doi.org/10.1080/10408398.2011.644353.Suche in Google Scholar PubMed
48. Zhao, Q, Xiong, H, Selomulya, C, Chen, XD, Zhong, H, Wang, S, et al.. Enzymatic hydrolysis of rice dreg protein: effects of enzyme type on the functional properties and antioxidant activities of recovered proteins. Food Chem 2012;134:1360–7. https://doi.org/10.1016/j.foodchem.2012.03.033.Suche in Google Scholar PubMed
49. Jamdar, SN, Rajalakshmi, V, Pednekar, MD, Juan, F, Yardi, V, Sharma, A. Influence of degree of hydrolysis on functional properties, antioxidant activity and ace inhibitory activity of peanut protein hydrolysate. Food Chem 2010;121:178–84. https://doi.org/10.1016/j.foodchem.2009.12.027.Suche in Google Scholar
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Articles
- Orange juice ultrafiltration: characterisation of deposit layers and membrane surfaces after fouling and cleaning
- Changes in volatile organic compounds and lipid oxidation in traditional Chinese bacon during cold smoking
- Drying of pineapple slices using combined low-pressure superheated steam and vacuum drying
- An energy dispersive X-ray fluorescence spectrometry approach for the identification of geographical origin of wheat flour
- Study on quality change mechanism of green turnip slices during low pressure superheated steam drying based on sensitivity analysis method
- Aqueous enzymatic extraction of peanut oil body and protein and evaluation of its physicochemical and functional properties
- Effect of microwave, infrared, and convection hot-air on drying kinetics and quality properties of okra pods
Artikel in diesem Heft
- Frontmatter
- Articles
- Orange juice ultrafiltration: characterisation of deposit layers and membrane surfaces after fouling and cleaning
- Changes in volatile organic compounds and lipid oxidation in traditional Chinese bacon during cold smoking
- Drying of pineapple slices using combined low-pressure superheated steam and vacuum drying
- An energy dispersive X-ray fluorescence spectrometry approach for the identification of geographical origin of wheat flour
- Study on quality change mechanism of green turnip slices during low pressure superheated steam drying based on sensitivity analysis method
- Aqueous enzymatic extraction of peanut oil body and protein and evaluation of its physicochemical and functional properties
- Effect of microwave, infrared, and convection hot-air on drying kinetics and quality properties of okra pods