Novelty of Penicillium camembertii Lipase Supported on Glutaraldehyde Activated-SBA-15 Mesoporous Silica for Mono-Esterification of Bioglycerol in Non-Aqueous Media
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Moreshwar P. Hude
Abstract
Hexagonal mesoporous type silica SBA-15 with pore sizes in the range 5.0–8.3 nm was synthesized using non-ionic triblock copolymer and characterized by Accelerated Surface Area Porosimetry (ASAP), FT-IR spectroscopy, X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Different lipases were immobilized in glutaraldehyde activated mesoporous SBA-15 support. The resulting supported enzymes were shown to be active and stable catalysts for esterification of glycerol with oleic acid to produce monoglyceride (MG) which is commonly used in food industry. Various parameters were studied systematically to study kinetics. MG Synthesis using enzymatic process is an environmentally friendly approach. Enzyme immobilized on SBA-15 showed the best stability and catalytic activity in organic solvents. Out of various lipases studied penicillium camembertii (Lipase G) produced MG efficiently at low temperature. Reusability was studied on immobilized enzymes. Immobilized lipase maintained 90 % of its esterification activity in non-aqueous media even after 4 cycles of use. The selectivity of Lipase G is found to be 98 % for monoacylglyceride.
Funding statement: Funding: Department of Biotechnology, Govt of India; Graduate Exchange Programme, University of Saskatchewan, Canada
Acknowledgements
G.D.Y. received support from R.T. Mody Distinguished Professor Endowment and J. C. Bose National Fellowship of Department of Science and Technology, Government of India. MPH thanks Department of Biotechnology (DBT) Govt. of India for Research fellowship and Graduate Students Exchange programme, University of Saskatchewan, Canada. We thank Amano Enzymes (Japan) for free enzyme samples.
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Supplemental Material
The online version of this article (DOI: 10.1515/ijcre-2014-0058) offers supplementary material, available to authorized users.
©2016 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Editorial
- In Honour of Professor Serge Kaliaguine
- Research Articles
- Adsorptive Removal of Nitrogen and Sulfur Containing Compounds by SBA15 Supported Nickel (II) and Tungsten Phosphides and the Adsorption Mechanisms
- Study of Cu-Zn and Au/TiO2 Catalysts on Anodized Aluminum Monoliths for Hydrogen Generation and Purification
- Electrocatalytic Activity of Three Carbon Materials for the In-situ Production of Hydrogen Peroxide and Its Application to the Electro-Fenton Heterogeneous Process
- Graphitic Carbon Nitride-Titanium Dioxide Nanocomposite for Photocatalytic Hydrogen Production under Visible Light
- Simulation of the Selective Hydrogenation of C3-Cut in the Liquid Phase
- On a Rational Performance Evaluation for the Development of Inorganic Membrane Technology in Gas Separation and Membrane Reactors
- Equilibrium and Kinetics of Methane and Ethane Adsorption in Carbon Molecular Sieve
- Synthesis of Mesoporous Tungsten Oxide/γ-Alumina and Surfactant-Capped Tungsten Oxide Nanoparticles and Their Catalytic Activities in Oxidative Cleavage of Oleic Acid
- Comparative Study of Quick Lime and CaO as Catalysts of Safflower Oil Transesterification
- Novelty of Penicillium camembertii Lipase Supported on Glutaraldehyde Activated-SBA-15 Mesoporous Silica for Mono-Esterification of Bioglycerol in Non-Aqueous Media
- Kinetics of Transesterification of Safflower Oil to Obtain Biodiesel Using Heterogeneous Catalysis
- Whole Cell Bioconversion of (+)-valencene to (+)-nootkatone in 100 % Organic Phase using Yarrowia lipolytica 2.2ab
Artikel in diesem Heft
- Frontmatter
- Editorial
- In Honour of Professor Serge Kaliaguine
- Research Articles
- Adsorptive Removal of Nitrogen and Sulfur Containing Compounds by SBA15 Supported Nickel (II) and Tungsten Phosphides and the Adsorption Mechanisms
- Study of Cu-Zn and Au/TiO2 Catalysts on Anodized Aluminum Monoliths for Hydrogen Generation and Purification
- Electrocatalytic Activity of Three Carbon Materials for the In-situ Production of Hydrogen Peroxide and Its Application to the Electro-Fenton Heterogeneous Process
- Graphitic Carbon Nitride-Titanium Dioxide Nanocomposite for Photocatalytic Hydrogen Production under Visible Light
- Simulation of the Selective Hydrogenation of C3-Cut in the Liquid Phase
- On a Rational Performance Evaluation for the Development of Inorganic Membrane Technology in Gas Separation and Membrane Reactors
- Equilibrium and Kinetics of Methane and Ethane Adsorption in Carbon Molecular Sieve
- Synthesis of Mesoporous Tungsten Oxide/γ-Alumina and Surfactant-Capped Tungsten Oxide Nanoparticles and Their Catalytic Activities in Oxidative Cleavage of Oleic Acid
- Comparative Study of Quick Lime and CaO as Catalysts of Safflower Oil Transesterification
- Novelty of Penicillium camembertii Lipase Supported on Glutaraldehyde Activated-SBA-15 Mesoporous Silica for Mono-Esterification of Bioglycerol in Non-Aqueous Media
- Kinetics of Transesterification of Safflower Oil to Obtain Biodiesel Using Heterogeneous Catalysis
- Whole Cell Bioconversion of (+)-valencene to (+)-nootkatone in 100 % Organic Phase using Yarrowia lipolytica 2.2ab
