Whole Cell Bioconversion of (+)-valencene to (+)-nootkatone in 100 % Organic Phase using Yarrowia lipolytica 2.2ab
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Dulce M. Palmerín-Carreño
Abstract
The aim of this work was to assess the whole cell bioconversion of (+)-valencene to (+)-nootkatone in 100 % organic phase (orange essential oil) using a stirred tank bioreactor. Yarrowia lipolytica 2.2ab was used to perform bioconversion experiments; 600 mL of orange essential oil was inoculated with 50 cm3 of cell paste containing 13.5 g of biomass previously permeabilized with 0.2 % (w/v) of cetyl trimethylammonium bromide (CTAB) and enriched with 2.0 mM niacin. Experiments were conducted at 200 rpm, 0.5 vvm and 30 °C. The highest (+)-nootkatone yield was ca. 773 mg L−1 after 4 days of conversion. Bioconversion percent and volumetric productivity increased to 82.3 % and 8.06 mg L−1 h−1 compared to those reported previously using a three-phase partitioning bioreactor. The absence of free water in the system did not affect the performance of Y. lipolytica 2.2ab.
Funding statement: The authors are grateful for financial support from the Mexican Council of Science and Technology (CONACyT) for PhD Grant 22691.
References
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©2016 by De Gruyter
Articles in the same Issue
- Frontmatter
- Editorial
- In Honour of Professor Serge Kaliaguine
- Research Articles
- Adsorptive Removal of Nitrogen and Sulfur Containing Compounds by SBA15 Supported Nickel (II) and Tungsten Phosphides and the Adsorption Mechanisms
- Study of Cu-Zn and Au/TiO2 Catalysts on Anodized Aluminum Monoliths for Hydrogen Generation and Purification
- Electrocatalytic Activity of Three Carbon Materials for the In-situ Production of Hydrogen Peroxide and Its Application to the Electro-Fenton Heterogeneous Process
- Graphitic Carbon Nitride-Titanium Dioxide Nanocomposite for Photocatalytic Hydrogen Production under Visible Light
- Simulation of the Selective Hydrogenation of C3-Cut in the Liquid Phase
- On a Rational Performance Evaluation for the Development of Inorganic Membrane Technology in Gas Separation and Membrane Reactors
- Equilibrium and Kinetics of Methane and Ethane Adsorption in Carbon Molecular Sieve
- Synthesis of Mesoporous Tungsten Oxide/γ-Alumina and Surfactant-Capped Tungsten Oxide Nanoparticles and Their Catalytic Activities in Oxidative Cleavage of Oleic Acid
- Comparative Study of Quick Lime and CaO as Catalysts of Safflower Oil Transesterification
- Novelty of Penicillium camembertii Lipase Supported on Glutaraldehyde Activated-SBA-15 Mesoporous Silica for Mono-Esterification of Bioglycerol in Non-Aqueous Media
- Kinetics of Transesterification of Safflower Oil to Obtain Biodiesel Using Heterogeneous Catalysis
- Whole Cell Bioconversion of (+)-valencene to (+)-nootkatone in 100 % Organic Phase using Yarrowia lipolytica 2.2ab
Articles in the same Issue
- Frontmatter
- Editorial
- In Honour of Professor Serge Kaliaguine
- Research Articles
- Adsorptive Removal of Nitrogen and Sulfur Containing Compounds by SBA15 Supported Nickel (II) and Tungsten Phosphides and the Adsorption Mechanisms
- Study of Cu-Zn and Au/TiO2 Catalysts on Anodized Aluminum Monoliths for Hydrogen Generation and Purification
- Electrocatalytic Activity of Three Carbon Materials for the In-situ Production of Hydrogen Peroxide and Its Application to the Electro-Fenton Heterogeneous Process
- Graphitic Carbon Nitride-Titanium Dioxide Nanocomposite for Photocatalytic Hydrogen Production under Visible Light
- Simulation of the Selective Hydrogenation of C3-Cut in the Liquid Phase
- On a Rational Performance Evaluation for the Development of Inorganic Membrane Technology in Gas Separation and Membrane Reactors
- Equilibrium and Kinetics of Methane and Ethane Adsorption in Carbon Molecular Sieve
- Synthesis of Mesoporous Tungsten Oxide/γ-Alumina and Surfactant-Capped Tungsten Oxide Nanoparticles and Their Catalytic Activities in Oxidative Cleavage of Oleic Acid
- Comparative Study of Quick Lime and CaO as Catalysts of Safflower Oil Transesterification
- Novelty of Penicillium camembertii Lipase Supported on Glutaraldehyde Activated-SBA-15 Mesoporous Silica for Mono-Esterification of Bioglycerol in Non-Aqueous Media
- Kinetics of Transesterification of Safflower Oil to Obtain Biodiesel Using Heterogeneous Catalysis
- Whole Cell Bioconversion of (+)-valencene to (+)-nootkatone in 100 % Organic Phase using Yarrowia lipolytica 2.2ab
