High-resolution capillary electrophoresis for the determination of carbamylated albumin
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Julien Favresse
und
Joris Delanghe
Abstract
Objectives
Carbamylation is a non-enzymatic post-translational reaction of a primary amino group of a protein with isocyanate. The albumin carbamylation is a negative prognostic factor in chronic kidney disease (CKD) patients and induce charge difference implying an observed shift in electrophoretic mobility that can be measured through a symmetry factor (SF).
Methods
The Helena V8 and the Sebia Capillarys 2 systems were used for all experiments. The effect of in vitro carbamylation on the SF by spiking increasing concentrations of potassium isocyanate (KCNO) in serum of three healthy volunteers was investigated. Theoretical plate numbers (N) as a surrogate of separation efficiency were also calculated and correlations between SF and renal function biomarkers were performed on 284 patients.
Results
A dose-dependent impact of KCNO on the SF was observed for both methods with the Helena V8 being more sensitive. The mean N was significantly higher on the Helena V8 as compared to the Sebia Capillarys 2 (2,972 vs. 444.1, p<0.0001). The SF correlated significantly with eGFR (r=0.50, p<0.0001), creatinine (r=−0.31, p<0.0001) and urea (r=−0.34, p<0.0001) on the Helena V8. On the Sebia Capillarys 2, a significant correlation was only observed with eGFR (r=0.17, p=0.004). A better discrimination between CKD stages was also observed using the Helena V8.
Conclusions
Thanks to a higher mean N, the Helena V8 might offer new possibilities, including detection of carbamylated albumin through SF calculation. Further studies are still needed to confirm the interest of using this type of assays in clinical routine.
Acknowledgments
The authors thanks Mr Thierry De Bruyne for performing the Adobe InDesign analysis and Mr Guillaume Sondag for performing the spiking experiment.
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Research funding: None declared.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from the three healthy volunteers included in this study.
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Ethical approval: The local Institutional Review Board approved the study exempt from review.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/cclm-2021-0926).
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
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Artikel in diesem Heft
- Frontmatter
- Editorial
- The clinical value of assessing the inter-method bias: the lesson from prostate specific antigen measurement
- Mini Review
- Methods to reduce lipemic interference in clinical chemistry tests: a systematic review and recommendations
- Opinion Paper
- Troponin interference with special regard to macrocomplex formation
- Guidelines and Recommendations from Scientific Societies
- Use of high-sensitivity cardiac troponins in the emergency department for the early rule-in and rule-out of acute myocardial infarction without persistent ST-segment elevation (NSTEMI) in Italy
- Genetics and Molecular Diagnostics
- Effect of preexamination conditions in a centralized-testing model of non-invasive prenatal screening
- General Clinical Chemistry and Laboratory Medicine
- Comparative study of human growth hormone measurements: impact on clinical interpretation
- Establishing pre-analytical requirements and maximizing peptide recovery in the analytical phase for mass spectrometric quantification of amyloid-β peptides 1–42 and 1–40 in CSF
- Validation of the LUMIPULSE automated immunoassay for the measurement of core AD biomarkers in cerebrospinal fluid
- Targeted profiling of 24 sulfated and non-sulfated bile acids in urine using two-dimensional isotope dilution UHPLC-MS/MS
- High-resolution capillary electrophoresis for the determination of carbamylated albumin
- Real-time monitoring of drug laboratory test interactions: a proof of concept
- Afamin predicts the prevalence and incidence of nonalcoholic fatty liver disease
- Reference Values and Biological Variations
- Blood sampling frequency as a proxy for comorbidity indices when identifying patient samples for review of reference intervals
- Coagulation parameters in the newborn and infant – the Copenhagen Baby Heart and COMPARE studies
- Hematology and Coagulation
- Policies and practices in the field of laboratory hematology in Croatia – a current overview and call for improvement
- Cardiovascular Diseases
- Evaluation of the Atellica TnIH cardiac troponin I assay and assessment of biological equivalence
- Infectious Diseases
- Inadequate design of mutation detection panels prevents interpretation of variants of concern: results of an external quality assessment for SARS-CoV-2 variant detection
- Letters to the Editors
- The pronounced decline of anti-SARS-CoV-2 spike trimeric IgG and RBD IgG in baseline seronegative individuals six months after BNT162b2 vaccination is consistent with the need for vaccine boosters
- ACE polymorphism is a determinant for COVID-19 mortality in the post-vaccination era
- A look at the precision, sensitivity and specificity of SARS-CoV-2 RT-PCR assays through a dedicated external quality assessment round
- Value of hypocalcemia and thromboinflammatory biomarkers for prediction of COVID-19 severity during the second wave: were all the waves the same?
- Metagenomic next-generation sequencing (mNGS) confirmed a critical case of severe fever with thrombocytopenia syndrome virus (SFTSV)
- Biotin interference: evaluation of an updated thyroglobulin electrochemiluminescent immunoassay
- Evaluation of the Beckman Coulter Access Procalcitonin Assay: analytical and clinical performance
- Analytical performance evaluation of the new sST2 turbidimetric assay implemented in laboratory automation systems
- Pre-analytical recommendations and reference values for circulating calprotectin are sample type and assay dependent
- Congress Abstracts
- Annual Meeting of the Royal Belgian Society of Laboratory Medicine: “Women’s health: from puberty to menopause”
