Diagnostic performances of clinical laboratory tests using Triton X-100 to reduce the biohazard associated with routine testing of Ebola virus-infected patients
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Massimo Tempestilli
Abstract
Background: Ebola virus, an enveloped virus, is the cause of the largest and most complex Ebola virus disease (EVD) outbreak in West Africa. Blood or body fluids of an infected person may represent a biohazard to laboratory workers. Laboratory tests of virus containing specimens should be conducted in referral centres at biosafety level 4, but based on the severity of clinical symptoms, basic laboratories might be required to execute urgent tests for patients suspected of EVD. The aim of this work was to compare the analytical performances of laboratory tests when Triton X-100, a chemical agent able to inactivate other enveloped viruses, was added to specimens.
Methods: Results of clinical chemistry, coagulation and haematology parameters on samples before and after the addition of 0.1% (final concentration) of Triton X-100 and 1 h of incubation at room temperature were compared.
Results: Overall, results showed very good agreement by all statistical analyses. Triton X-100 at 0.1% did not significantly affect the results for the majority of the analytes tested.
Conclusions: Triton X-100 at 0.1% can be used to reduce the biohazard in performing laboratory tests on samples from patients with EVD without affecting clinical decisions.
Acknowledgments
The authors thank the entire Biochemistry and Pharmacology Laboratory staff. In particular, they are grateful to Basile MR, Gagliardi C, Giuffrè S, Luisi G and Martino G for their technical help. Authors wish to thank Baker A for language assistance.
Author contributions:All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Financial support: This study was supported by grants from Ministero della Salute of Italy (Istituto Nazionale per le Malattie Infettive I.R.C.C.S. “Lazzaro Spallanzani”, Ricerca Corrente).
Employment or leadership:None declared.
Honorarium: None declared.
Competing interests:The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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Supplemental Material:
The online version of this article (DOI: 10.1515/cclm-2015-0119) offers supplementary material, available to authorized users.
©2015 by De Gruyter
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Artikel in diesem Heft
- Frontmatter
- Editorial
- Biomarkers of inflammatory bowel disease: ready for prime time?
- Review
- Discriminant indices for distinguishing thalassemia and iron deficiency in patients with microcytic anemia: a meta-analysis
- Mini Reviews
- Cell-free DNA for diagnosing myocardial infarction: not ready for prime time
- The Laboratory Medicine and the care of patients infected by the Ebola virus. Experience in a reference hospital of Madrid, Spain
- Opinion Papers
- Theranos phenomenon − part 2
- Considerations in parathyroid hormone testing
- Quantity quotient reporting. Comparison of various models
- Genetics and Molecular Diagnostics
- Assessing quality and functionality of DNA isolated from FFPE tissues through external quality assessment in tissue banks
- Influence of storage conditions and extraction methods on the quantity and quality of circulating cell-free DNA (ccfDNA): the SPIDIA-DNAplas External Quality Assessment experience
- Non-invasive fetal ABO genotyping in maternal plasma using real-time PCR
- Screening non-deletion α-thalassaemia mutations in the HBA1 and HBA2 genes by high-resolution melting analysis
- General Clinical Chemistry and Laboratory Medicine
- European views on patients directly obtaining their laboratory test results
- Diagnostic performances of clinical laboratory tests using Triton X-100 to reduce the biohazard associated with routine testing of Ebola virus-infected patients
- Thrombin generation, D-dimer and protein S in uncomplicated pregnancy
- Multi-analyte analysis of non-vitamin K antagonist oral anticoagulants in human plasma using tandem mass spectrometry
- Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing
- Faecal leukocyte esterase activity is an alternative biomarker in inflammatory bowel disease
- Reference Values and Biological Variations
- Total folate and 5-methyltetrahydrofolate in the cerebrospinal fluid of children: correlation and reference values
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- Infectious Diseases
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- The impact of Tween 20 on repeatability of amyloid β and tau measurements in cerebrospinal fluid
- Effect of storage time and temperature on the generation of reactive oxygen species in peripheral blood leukocytes
- Iohexol interference in the α2-globulin fraction of the serum protein capillary electrophoresis
- A functional variant in the γ-glutamyltransferase (GGT)1 gene is associated with airflow obstruction in smokers
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