Startseite Characterization of the complete mitochondrial genome of Metastrongylus salmi (M. salmi) derived from Tibetan pigs in Tibet, China
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Characterization of the complete mitochondrial genome of Metastrongylus salmi (M. salmi) derived from Tibetan pigs in Tibet, China

  • Kun Li , Muhammad Shahzad , Hui Zhang , Khalid Mehmood , Xiong Jiang , Houqiang Luo , Lihong Zhang , Xiaoqian Dong und Jiakui Li EMAIL logo
Veröffentlicht/Copyright: 13. April 2018
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Acta Parasitologica
Aus der Zeitschrift Acta Parasitologica Band 63 Heft 2

Abstract

The present study was designed to determine and analyze the mt genomes of Metastrongylus salmi (M. salmi), and reveal the phylogenetic relationships of this parasite using mt DNA sequences. Results showed that the complete mt genome of M. salmi was 13722 bp containing 12 protein-coding genes (cox1-3, nad1-6, nad4L, atp6 and cytb), 22 transfer RNA genes, and 2 ribosomal RNA genes (rrnL and rrnS). The overall A+T content was 73.54% and the nucleotide composition was A (23.52%), C (6.14%), G (19.60%), T (50.02%), and N (UCAG) (0.73%). A total of 4237 amino acids are encoded from the Tibetan isolates of M. salmi mt genomes. The ATA was predicted as the most common starting codon with 41.7% (5/12 protein genes); and 11 of the 12 protein genes were found to have a TAG or TAA translation termination codon. By clustering together the phylogenetic trees of Tibetan M. salmi and Austrian M. salmi, the M. salmi isolated from Tibetan pigs was found to be highly homological with that stemmed from Austrian one. This information provides meaningful insights into the phylogenetic position of the M. salmi China isolate and represents a useful resource for selecting molecular markers for diagnosis and population studies.

Keywords: Lungworm; Tibetan pigs; mitochondrial genome; Metastrongylus salmi

Acknowledgments

The current research was supported by the Key Science Fund of Science and Technology Agency of Tibet Autonomous Region and projects in the National Science & Technology Pillar Program during the 12th Five-year Plan Period (2012BA D3B03). Kun Li is supported by China Scholarship Council under Grant No. 201706760018.

  1. Conflict of interest: The authors state that there are no competing interests.

  2. Ethics statement: All procedures were performed in accordance with the laws, regulations, and strict guidelines of the Laboratory Animals Research Centre of Hubei province, P. R. China. Samples were collected with the permission of the relevant institutions and by following the parameters as set by the ethics committee of Huazhong Agricultural University (Permit number: 4200695757).

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Received: 2017-7-12
Revised: 2017-12-29
Accepted: 2018-1-18
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2018-0032
Schlagwörter für diesen Artikel
Lungworm; Tibetan pigs; mitochondrial genome; Metastrongylus salmi

Artikel in diesem Heft

  2. Morphological, histological and molecular characterization of Myxobolus kingchowensis and Thelohanellus cf. sinensis infecting gibel carp Carassius auratus gibelio (Bloch, 1782)
  4. Cystic echinococcosis in animals and humans of Maharashtra State, India
  6. Structural predication and antigenic analysis of Toxoplasma gondii ROP20
  8. Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice
  10. A new species of Cacatuocotyle (Monogenea, Dactylogyridae) parasitizing Astyanax spp. (Characiformes, Characidae) from Brazil, including molecular data and a key to species identification
  12. Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters
  14. Characterization of the complete mitochondrial genome of Metastrongylus salmi (M. salmi) derived from Tibetan pigs in Tibet, China
  16. Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
  18. Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences
  20. Patterns of the parasite communities in a fish assemblage of a river in the Brazilian Amazon region
  22. Histological description of Cercaria etgesiiShchenkov, 2017 daughter sporocysts (Trematoda: Pleurogenidae): compartmentalization of the brood cavity and germinal mass morphology
  24. Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China
  26. First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt
  28. Prevalence and risk factors associated with Tritrichomonas foetus infection in cattle in the state of Paraíba, Brazil
  30. The development of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) in its intermediate host Cypridopsis vidua in Brazil
  32. Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction
  34. Evaluation of the protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing BmAMA1 as vaccines against Babesia microti infection in hamster
  36. A new species of Chondracanthus (Cyclopoida: Chondracanthidae) parasitic on deep-sea Dibranchus spongiosa(Lophiiformes: Ogcocephalidae) from the Eastern Central Pacific
  38. Evaluation of four commercial DNA extraction kits for the detection of Microsporidia and the importance of pretreatments in DNA isolation
  40. Presence of Acanthamoeba in the ocular surface in a Spanish population of contact lens wearers
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  47. Case report
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  49. Case report
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  53. Erratum
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