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Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters

  • Fabiola del Rocío Villalobos-Gómez , Mario García-Lorenzana , Galileo Escobedo , Patricia Talamás-Rohana , Rogelio Salinas-Gutiérrez , Verónica-Ivonne Hernández-Ramírez , Esperanza Sánchez-Alemán , María del Rosario Campos-Esparza , Martín Humberto Muñoz-Ortega and Javier Ventura-Juárez EMAIL logo
Published/Copyright: April 13, 2018
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Acta Parasitologica
From the journal Acta Parasitologica Volume 63 Issue 2

Abstract

The neuroimmunoregulation of inflammation has been well characterized. Entamoeba histolytica provokes an inflammatory response in the host in which macrophages and neutrophils are the first line of defense. The aim of this study was to analyze the effect of the 220 kDa lectin of Entamoeba histolytica on stimulation of human macrophages and neutrophils, especially the secretion of cytokines and the relation of these to neurotransmitters. Human cells were interacted with L220, epinephrine, nicotine, esmolol and vecuronium bromide. The concentrations of IL-1β, IFN-γ, TNF-α and IL-10 were determined by ELISA at, 4 h of interaction. L220 has a cytokine stimulating function of macrophages and neutrophils for secretion of IL-1β, and IL-10 only by macrophages, which was modulated by the effect of vecuronium on cholinergic receptors in this immune cells.

Keywords: L220 Entamoeba histolytica; neutrophils; macrophages; epinephrine; esmolol; nicotine; vecuronium bromide; cytokines

  1. Conflict of interest

    Conflict of interest statement: The authors have not interest conflict

Acknowledgment

This work was financed by a grant (number PIBB11-3) from the Universidad Autónoma de Aguascalientes and a basic science grant (number 134487) from CONACYT, as well as by a CONACYT fellowship (number 231571) for Villalobos-Gómez FR as part of the PhD program (Doctorado en Ciencias Biológicas y de la Salud) of the Universidad Autónoma Metropolitana

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Received: 2017-7-4
Revised: 2017-12-13
Accepted: 2018-1-17
Published Online: 2018-4-13
Published in Print: 2018-6-26

© 2018 W. Stefański Institute of Parasitology, PAS

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https://doi.org/10.1515/ap-2018-0031
Keywords for this article
L220 Entamoeba histolytica; neutrophils; macrophages; epinephrine; esmolol; nicotine; vecuronium bromide; cytokines

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  2. Morphological, histological and molecular characterization of Myxobolus kingchowensis and Thelohanellus cf. sinensis infecting gibel carp Carassius auratus gibelio (Bloch, 1782)
  4. Cystic echinococcosis in animals and humans of Maharashtra State, India
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  8. Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice
  10. A new species of Cacatuocotyle (Monogenea, Dactylogyridae) parasitizing Astyanax spp. (Characiformes, Characidae) from Brazil, including molecular data and a key to species identification
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  16. Balantioides coli: morphological and ultrastructural characteristics of pig and non-human primate isolates
  18. Genetic diversity of Taenia hydatigena in the northern part of the West Bank, Palestine as determined by mitochondrial DNA sequences
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  22. Histological description of Cercaria etgesiiShchenkov, 2017 daughter sporocysts (Trematoda: Pleurogenidae): compartmentalization of the brood cavity and germinal mass morphology
  24. Isolation, identification and characterization of the nematophagous fungus Arthrobotrys (Monacrosporium) sinense from China
  26. First molecular characterization and morphological aspects of Sarcocystis fusiformis infecting water buffalo Bubalus bubalis in Egypt
  28. Prevalence and risk factors associated with Tritrichomonas foetus infection in cattle in the state of Paraíba, Brazil
  30. The development of Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae) in its intermediate host Cypridopsis vidua in Brazil
  32. Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction
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