Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Age-related changes of plasma glycosaminoglycans
-
Katarzyna B. Komosińska-Vassev
Veröffentlicht/Copyright:
13. Dezember 2007
Abstract
Background: This study was undertaken to reveal how the physiological ageing process influences plasma glycosaminoglycans (GAGs) content and composition.
Methods: GAGs isolated from plasma samples obtained from 105 healthy subjects were quantified using a hexuronic acid assay and subjected to electrophoretic fractionation.
Results: We found that total GAGs decreased gradually with advancing age. The electrophoretic analysis allowed identifying chondroitin sulfates, dermatan sulfates and heparan sulfates and heparin in plasma of healthy individuals. Chondroitin sulfates, predominant plasma GAGs, showed declining values with age and displayed higher structural diversity in subjects from the first five decades of life compared to older subjects. An age-related increase in plasma dermatan sulfates content during the first four decades of life followed by a decrease in a later period of life was apparent. Structural heterogeneity of these glycans was found in subjects until 10 years of age. Heparan sulfates and heparin plasma levels were inversely correlated with age until 25 years of age, without age-dependent changes thereafter. These macromolecules displayed higher heterogeneity of structure in subjects over 50 years of age than in younger subjects.
Conclusions: The obtained results indicate that age should be taken into account in all studies investigating blood GAGs alterations during pathological conditions.
Clin Chem Lab Med 2008;46:219–24.
Keywords: ageing process; chondroitin sulfate; dermatan sulfate; extracellular matrix; heparin sulfate/heparin; plasma glycosaminoglycans
Received: 2007-8-31
Accepted: 2007-10-19
Published Online: 2007-12-13
Published in Print: 2008-02-01
©2008 by Walter de Gruyter Berlin New York
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Opinion Papers
- The double whammy of endogenous insulin antibodies in non-diabetic subjects
- ‘Likelihood-ratio’ and ‘odds’ applied to monitoring of patients as a supplement to ‘reference change value’ (RCV)
- Genetics and Molecular Diagnostics
- A family with multiple mutations and sequence variations in the α- and β-globin gene clusters
- CTLA-4 polymorphism 49A–G is associated with placental abruption and preeclampsia in Finnish women
- Rare TA repeats in promoter TATA box of the UDP glucuronosyltranferase (UGT1A1) gene in Croatian subjects
- Association of high sensitive C-reactive protein with apolipoprotein E polymorphism in children and young adults: The Cardiovascular Risk in Young Finns Study
- Relationship between thiolactonase activity and hyperhomocysteinemia according to MTHFR gene polymorphism in Tunisian Behçet's disease patients
- A rapid and sensitive method to detect specific human lymphocyte antigen (HLA) class II alleles associated with celiac disease
- General Clinical Chemistry and Laboratory Medicine
- Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods
- Long-term prognostic value of midregional pro-adrenomedullin and C-terminal pro-endothelin-1 in patients with acute myocardial infarction
- Changes in serum fetuin-A and inflammatory markers levels in end-stage renal disease (ESRD): effect of a single session haemodialysis
- Modeling the variability of creatinine measurements improves estimates of the glomerular filtration rate
- Reference Values
- Age-related changes of plasma glycosaminoglycans
- Stability of heparin blood samples during transport based on defined pre-analytical quality goals
- Comparison of creatinine-based estimations of glomerular filtration rate in endurance athletes at rest
- Assay-dependent variability of serum insulin levels during oral glucose tolerance test: influence on reference intervals for insulin and on cut-off values for insulin sensitivity indices
- N-Terminal proB-type natriuretic peptide (NT-proBNP) concentrations in elite rugby players at rest and after active and passive recovery following strenuous training sessions
- Strenuous exercise activates growth factors and chemokines over-expression in human serum of top-level triathlon athletes during a competitive season
- Validation and Outcome Studies
- Usefulness of six non-proprietary indirect markers of liver fibrosis in patients with chronic hepatitis C
- Evaluation of a new Sebia isoelectrofocusing kit for α1-antitrypsin phenotyping with the Hydrasys® System
- Different biological matrices (serum and plasma) utilization in consolidation processes: evaluation of seven Access® immunoassays
- Evaluation of the GEM® Premier™ 4000: a compact blood gas CO-Oximeter and electrolyte analyzer for point-of-care and laboratory testing
- Impact of storage conditions on genetic analysis or viral load determination in clinical specimens
- Homocysteine measurement by Vitros® Microtip homocysteine assay
- Evaluation of a novel standardized system for collection and quantification of oral fluid
- Letters to the Editor
- Tumor necrosis factor-α G(–308)A promoter polymorphism, matrix metalloproteinase (MMP)-3 5A/6A gene variation, MMP-9 C(–1562)T promoter polymorphism and risk and extent of ischemic heart disease
- Polyethylene glycol interference in the Immulite prolactin assay: treatment of the calibrators with polyethylene glycol does not improve diagnostic accuracy
Schlagwörter für diesen Artikel
ageing process;
chondroitin sulfate;
dermatan sulfate;
extracellular matrix;
heparin sulfate/heparin;
plasma glycosaminoglycans
Artikel in diesem Heft
- Opinion Papers
- The double whammy of endogenous insulin antibodies in non-diabetic subjects
- ‘Likelihood-ratio’ and ‘odds’ applied to monitoring of patients as a supplement to ‘reference change value’ (RCV)
- Genetics and Molecular Diagnostics
- A family with multiple mutations and sequence variations in the α- and β-globin gene clusters
- CTLA-4 polymorphism 49A–G is associated with placental abruption and preeclampsia in Finnish women
- Rare TA repeats in promoter TATA box of the UDP glucuronosyltranferase (UGT1A1) gene in Croatian subjects
- Association of high sensitive C-reactive protein with apolipoprotein E polymorphism in children and young adults: The Cardiovascular Risk in Young Finns Study
- Relationship between thiolactonase activity and hyperhomocysteinemia according to MTHFR gene polymorphism in Tunisian Behçet's disease patients
- A rapid and sensitive method to detect specific human lymphocyte antigen (HLA) class II alleles associated with celiac disease
- General Clinical Chemistry and Laboratory Medicine
- Probing the redox activity of T-lymphocytes deposited at electrode surfaces with voltammetric methods
- Long-term prognostic value of midregional pro-adrenomedullin and C-terminal pro-endothelin-1 in patients with acute myocardial infarction
- Changes in serum fetuin-A and inflammatory markers levels in end-stage renal disease (ESRD): effect of a single session haemodialysis
- Modeling the variability of creatinine measurements improves estimates of the glomerular filtration rate
- Reference Values
- Age-related changes of plasma glycosaminoglycans
- Stability of heparin blood samples during transport based on defined pre-analytical quality goals
- Comparison of creatinine-based estimations of glomerular filtration rate in endurance athletes at rest
- Assay-dependent variability of serum insulin levels during oral glucose tolerance test: influence on reference intervals for insulin and on cut-off values for insulin sensitivity indices
- N-Terminal proB-type natriuretic peptide (NT-proBNP) concentrations in elite rugby players at rest and after active and passive recovery following strenuous training sessions
- Strenuous exercise activates growth factors and chemokines over-expression in human serum of top-level triathlon athletes during a competitive season
- Validation and Outcome Studies
- Usefulness of six non-proprietary indirect markers of liver fibrosis in patients with chronic hepatitis C
- Evaluation of a new Sebia isoelectrofocusing kit for α1-antitrypsin phenotyping with the Hydrasys® System
- Different biological matrices (serum and plasma) utilization in consolidation processes: evaluation of seven Access® immunoassays
- Evaluation of the GEM® Premier™ 4000: a compact blood gas CO-Oximeter and electrolyte analyzer for point-of-care and laboratory testing
- Impact of storage conditions on genetic analysis or viral load determination in clinical specimens
- Homocysteine measurement by Vitros® Microtip homocysteine assay
- Evaluation of a novel standardized system for collection and quantification of oral fluid
- Letters to the Editor
- Tumor necrosis factor-α G(–308)A promoter polymorphism, matrix metalloproteinase (MMP)-3 5A/6A gene variation, MMP-9 C(–1562)T promoter polymorphism and risk and extent of ischemic heart disease
- Polyethylene glycol interference in the Immulite prolactin assay: treatment of the calibrators with polyethylene glycol does not improve diagnostic accuracy
