Startseite Membrane organisation and dynamics in the marine diatom Coscinodiscus wailesii (Bacillariophyceae)
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Membrane organisation and dynamics in the marine diatom Coscinodiscus wailesii (Bacillariophyceae)

  • Stefanie F. Kühn und Colin Brownlee
Veröffentlicht/Copyright: 5. Oktober 2005
Botanica Marina
Aus der Zeitschrift Band 48 Heft 4

Abstract

The membrane dye FM1-43 was used to visualise membrane internalisation in living cells of the marine centric diatoms Coscinodiscus wailesii and Thalassiosira punctigera. The results revealed dynamic and selective membrane trafficking and several cellular structures which have not been described in light microscopical studies. Intimate and regular contacts were found between the plasma membrane and the labiate processes and the inner face of the frustule in C. wailesii. The central cytoplasmic region close to the tonoplast and growing parts of the silica deposition vesicles became labelled, indicating that they were involved in membrane recycling. We never observed labelling of chloroplasts, nucleus or the vacuolar contents. These studies also revealed a novel perinuclear region that appeared not to show significant membrane turnover. When the cytoplasm was impaled with a micropipette, membranous material rapidly accumulated at the tip of the micropipette, presumably expelling it from the protoplast. This could be an effective defence mechanism against certain invasive pathogens, and further illustrates the highly dynamic nature of the endomembrane system.

:

Corresponding author

References

Aguado-Velasco, C. and M.S. Bretscher. 1999. Circulation of the plasma membrane in Dictyostelium. Mol. Biol. Cell10: 4419–4427.10.1091/mbc.10.12.4419Suche in Google Scholar PubMed PubMed Central

Battey, N., N. James, A. Greenland, A. Brownlee and C. Brownlee. 1999. Exocytosis and endocytosis. Plant Cell11: 643–659.10.1105/tpc.11.4.643Suche in Google Scholar PubMed PubMed Central

Carrol, A.D., C. Moyen, W.J.P. van Kesteren, F. Tooke, N.H. Battey and C. Brownlee. 1998. Ca2+, annexins and GTP modulate exocytosis from maize root cap protoplasts. Plant Cell10: 1267–1276.Suche in Google Scholar

Conner, S.D. and S.L. Schmid. 2003. Regulated portals of entry into the cell. Nature422: 37–44.10.1038/nature01451Suche in Google Scholar PubMed

Crawford, R.M. 1973. The organic component of the cell wall of the marine diatom Melosira nummuloides (Dillw.) C. Ag. Br. Phycol. J.8: 257–266.10.1080/00071617300650291Suche in Google Scholar

Guillard, R.R.L. and J.H. Ryther. 1962. Studies of marine planktonic diatoms. Can. J. Microbiol.8: 229–239.10.1139/m62-029Suche in Google Scholar PubMed

Hasle G.R. and C.B. Lange. 1989. Freshwater and brackish water Thalassiosira (Bacillariophyceae): taxa with tangentially undulated valves. Phycologia28: 120–135.10.2216/i0031-8884-28-1-120.1Suche in Google Scholar

Henkel, A.W., J. Lübke and A.J. Betz. 1996. FM1-43 dye ultrastructural localization in and release from frog motor nerve terminals. Proc. Natl. Acad. Sci.93: 1918–1923.10.1073/pnas.93.5.1918Suche in Google Scholar PubMed PubMed Central

Kubitscheck, U., U. Homann and G. Thiel. 2000. Osmotically evoked shrinking of guard-cell protoplasts causes vesicular retrieval of plasma membrane into the cytoplasm. Planta210: 423–431.10.1007/PL00008151Suche in Google Scholar PubMed

Kühn, S.F. 1996. Rhizamoeba schnepfii sp. nov., a naked amoeba feeding on marine diatoms (North Sea, German Bight). Arch. Protistenkd.147: 277–282.Suche in Google Scholar

Li, C.-W. and B.E. Volcani. 1985. Studies on the biochemistry and fine structure of silica shell formation in diatoms VIII. Morphogenesis of the cell wall in a centric diatom, Ditylum brightwellii. Protoplasma124: 10–29.Suche in Google Scholar

Low, P. and S. Chandra. 1994. Endocytosis in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.45: 609–631.10.1146/annurev.pp.45.060194.003141Suche in Google Scholar

Medlin, L.K., R.M. Crawford and R.A. Anderson. 1986. Histochemical and ultrastructural evidence for the function of the labiate process in the movement of centric diatoms. Br. Phycol. J.21: 297–301.10.1080/00071618600650351Suche in Google Scholar

Meyers, J.R., R.B. MacDonald, A. Duggan, D. Lenzi, D.G. Standaert, J.T. Corwin and D.P. Corey. 2003. Lighting up the senses: FM1-43 loading of sensory cells through nonselective ion channels. J. Neurosci.23: 4054–4065.10.1523/JNEUROSCI.23-10-04054.2003Suche in Google Scholar

Pickett-Heaps, J.D. 1998a. Cell division and morphogenesis of the centric diatom Chaetoceros decipiens (Bacillariophyceae) I. Living cells. J. Phycol.34: 989–994.10.1046/j.1529-8817.1998.340989.xSuche in Google Scholar

Pickett-Heaps, J.D. 1998b. Cell division and morphogenesis of the centric diatom Chaetoceros decipiens (Bacillariophyceae) II. Electron microscopy and a new paradigm for tip growth. J. Phycol.34: 995–1004.10.1046/j.1529-8817.1998.340995.xSuche in Google Scholar

Pickett-Heaps, J.D., A.-M.M. Schmid and L.A. Edgar. 1990. The cell biology of diatom valve formation. Prog. Phycological Res.7: 2–168.Suche in Google Scholar

Round, F.E., R.M. Crawford and D.G. Mann. 1990. The diatoms: biology and morphology of the genera. Cambridge University Press, Cambridge. pp. 747.Suche in Google Scholar

Schmid, A.-M.M. 1984a. Wall morphogenesis in Thalassiosira eccentrica: comparison of auxospore formation and the effect of MT-inhibitors. In: (D.G. Mann, ed.) Proceedings of the 7th International Diatom Symposium. Koeltz, Koenigstein. pp. 47–70.Suche in Google Scholar

Schmid, A.-M.M. 1984b. Tricornate spines in Thalassiosira eccentrica as a result of valve-modelling. In: (Mann, D.G., ed.) Proceedings of the 7th International Diatom Symposium. Koeltz, Koenigstein. pp. 71–95.Suche in Google Scholar

Schmid, A.-M.M. 1984c. Organization and function of cell-structures in diatoms and their morphogenesis. In: (M. Ricard, ed.) Proceedings of the 8th International Symposium on Diatoms. Koeltz, Koenigstein. pp. 271–292.Suche in Google Scholar

Schmid, A.-M.M. 1984d. Wall morphogenesis in Coscinodiscus wailesii Gran et Angst. II. Cytoplasmic events of valve morphogenesis. In: (M. Ricard, ed.) Proceedings of the 8th International Symposium on Diatoms. Koeltz, Koenigstein. pp. 293–314.Suche in Google Scholar

Schmid, A.-M.M. 1987. Morphogenetic forces in diatom cell wall formation. In: (J. Bereiter-Hahn, O.R. Anderson and W.-E. Reif, eds) Cytomechanics. Springer, Berlin. pp. 183–199.Suche in Google Scholar

Schmid, A.-M.M. 1988. The special Golgi-ER-mitochondrium unit in the diatom genus Coscinodiscus. Plant Syst. Evol.158: 211–223.10.1007/BF00936345Suche in Google Scholar

Schmid, A.-M.M. 1994. Aspects of morphogenesis and function of diatom cell walls with implication for taxonomy. Protoplasma181: 43–60.10.1007/978-3-7091-9378-5_3Suche in Google Scholar

Schmid, A.-M.M and D. Schultz. 1979. Wall morphogenesis in diatoms: deposition of silica by cytoplasmic vesicles. Protoplasma100: 267–288.10.1007/BF01279316Suche in Google Scholar

Schmid, A.-M.M. and B.E. Volcani. 1983. Wall morphogenesis in Coscinodiscus wailesii Gran & Angst. I. Valve morphology and development of its architecture. J. Phycol.19: 387–402.10.1111/j.0022-3646.1983.00387.xSuche in Google Scholar

Schmid, A.-M.M., R.K. Eberwein and M. Hesse. 1996. Pattern morphogenesis in cell walls of diatoms and pollen grains: a comparison. Protoplasma193: 144–173.10.1007/BF01276642Suche in Google Scholar

Schote, U. and J. Seelig. 1998. Interaction of the neuronal marker dye FM1-43 with lipid membranes: thermodynamics and lipid ordering. Biochim. Biophys. Acta1415: 135–146.10.1016/S0005-2736(98)00188-6Suche in Google Scholar

Smith, C.B. and W.J. Betz. 1996. Simultaneous independent measurement of endocytosis and exocytosis. Nature380: 531–534.10.1038/380531a0Suche in Google Scholar PubMed

van de Meene, A.M.L. and J.D. Pickett-Heaps. 2002. Valve morphogenesis in the centric diatom Proboscia alata Sundstrom. J. Phycol.38: 351–363.10.1046/j.1529-8817.2002.01124.xSuche in Google Scholar

von Stosch, H.A. 1981. Structural and histochemical observations on the organic layers of the diatom cell wall. In: (R. Ross, ed.) Proceedings of the 6th Diatom Symposium on Recent and Fossil Diatoms. Koeltz, Koenigstein. pp. 231–252.Suche in Google Scholar

Published Online: 2005-10-05
Published in Print: 2005-10-01

©2005 by Walter de Gruyter Berlin New York

Heruntergeladen am 23.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/BOT.2005.039/html
Button zum nach oben scrollen