Band 8, Heft 11

Major depression affects the central nervous system and thereafter the autonomic nervous system, immune system, and endocrine system. Na+/K+ ATPase, as a major mediator of cellular transmembrane ionic gradients, plays an important role in nervous signal transduction. Three types of Na+/K+ ATPase α subunit isoforms (ATP1A1, ATP1A2, and ATP1A3) are found in brain but vary in the type of cell and level of expression. It has been confirmed that reduced expression of ATP1A2 and ATP1A3 are related to depressive disorder. However, there is no reported correlation between ATP1A1 and major depression. This study investigated the potential correlation between ATP1A1 gene expression level and major depression. The expression levels of ATP1A1 gene in the peripheral circulation of both depressive patients and healthy human controls were quantified by using reverse transcripted quantitative polymerase chain reaction. Statistical analysis showed a significant decrease of ATP1A1 expression level in major depression patients when compared to that of healthy controls (P<0.01). The differences of gene nucleotide sequences and protein structures among ATP1A1, ATP1A2, and ATP1A3 were also illustrated. This study demonstrates, for the first time, that ATP1A1 gene expression level is significantly associated with major depression and suggests that ATP1A1 could be a significant molecular marker for diagnosis.

The effects of mercury (Hg), cadmium (Cd) and chromium (Cr) in concentrations ranging from 0.02 to 20 mg L−1 applied for 24 h were assessed in Lemna minor and Lemna gibba by measuring changes in protein concentration, ascorbic acid, phenolics, malondialdehyde (MDA), hydrogen peroxide (H2O2), the activity of guaiacol peroxidase (G-POX) and catalase (CAT). Ascorbic acid, phenolics, catalase and guaiacol peroxidase played a key role in the antioxidative response of L. gibba. Inadequate activity of antioxidant enzymes in the L. minor resulted in MDA and H2O2 accumulation. In both used species, Hg treatment decreased protein content and increased CAT and G-POX activity, but decreased MDA and H2O2 levels. Cadmium and chromium had opposite impacts on two used Lemna species on almost all observed parameters. Enhanced antioxidative responses of L. gibba to lower concentrations of Hg, Cd and Cr indicated greater abiotic stress tolerance than L. minor.

The purpose of this study is to provide new data on body composition in the Slovak population, particularly impedance vector components according to sex and age, relevant for bioelectrical impedance vector analysis (BIVA) in a clinical sample. The reference sample consisted of 1543 apparently healthy individuals (1007 females and 536 males), aged from 18 to 92 years and of 60 patients with Parkinson’s disease (PD) (26 females and 34 males), aged from 40 to 81 years. Bioelectrical parameters of resistance (R) and reactance (Xc) were measured with a monofrequency analyser (BIA 101). BIVA was used to analyse tissue electric properties in control subjects and patients with PD. The mean vector position differed significantly between PD patients and healthy controls in males of age subgroups 60–69 years and 70–79 years, respectively. These results were conterminous with significant Hotelling’s T2-test; 60–69 y T2=7.8, P=0.024 and 70–79 y T2=7.6, P=0.026. In the RXc-score graph three patients had values outside the 95% ellipse. Altered tissue electric properties were present in 23.5% of males and 15.4% of females. Distribution of impedance vector components in different age categories of healthy Slovak subjects are relevant to comparative population studies and to clinical practice.

Activation of Notch by Jagged-1 may plays a pivotal role in maturation of dendritic cells (DCs), but the mechanism has not been completely defined. In the present study, Hes-1 (Hairy/enhancer-of-split)-targeting siRNA was used to confirm a role of Jagged-1-Notch signaling pathway activation in maturation of murine bone marrow-derived DCs and to search for a target that plays a critical role. The results showed that compared with the control, lipopolysaccharide or Zymosan A groups, Jagged-1 (a soluble Jagged 1/Fc chimera protein) effectively increased expression of Hes-1 and Deltex-1 mRNA, which could be reversed by DAPT (2, 4-diamino-5-phenylthiazole), a specific inhibitor of the Notch signaling pathway. Hes-1-targeting siRNA could successfully down-regulate the endogenous Hes-1 expression in the DCs. Concurrently, a significant down-regulation of CD40, CD80, CD86 and MHC-II expressions on the surface of the DCs was found with the reduction of IL-12 yielded by the DCs. Our results demonstrate that Hes-1-targeting siRNA can inhibit the maturation of the DCs induced by Jagged-1, indicating Hes-1 may be an important target of Notch signaling mediating the maturation of DCs.

We investigated the influence of copper in a long-time treatment with concentrations of 0.05 mg L−1 and 0.1 mg L−1 to track the histopathological changes in gills of Carassius gibelio, and to find at what extent they will recover after the effect of the copper concentrations stops. Treatment with copper lasted 21 days and the recovery time was of the same duration. The results of histological examination showed degenerative changes (resulting in thinner secondary lamellae and filamentary epithelium), and hyperplastic and hypertrophic changes (proliferation, vasodilatation, aneurysms, epithelial interstitial edema, and fusion) in gills under the influence of two concentrations. The degenerative changes have higher prevalence at low concentrations, while hyperplastic and hypertrophic ones — at high concentrations. After the period of recovery they remained the same, but the extent of expression on the surface of gill filaments changed. The long-time copper intoxication in low concentrations of copper affects gill structure, causing severe changes whose recovery is a slow process that requires a longer period of time.

The aim of this study was to estimate the effect of agricultural landscape structure on the predation rate of red foxes Vulpes vulpes on grey partridges Perdix perdix during the breeding season and on their spatial relations. The number of partridge remains found around fox breeding dens (N=165) was used as an index of the predation rate in 10 study areas. Moreover, the distribution of both species and the searching intensity of partridge nesting habitat (permanent semi-natural vegetation) by foxes in relation to the landscape structure were studied using scat, track and call counts. The predation index (range 0.06–0.46 partridges/den) increased with spring partridge density and decreased with the occurrence of crop boundaries. The distribution of foxes and partridges in large fields was positively correlated with the occurrence of permanent vegetation, but no such effect was observed in small fields. The searching intensity in permanent vegetation by foxes decreased with the occurrence of these structures among small fields, but not among large fields. The study showed that in a differentiated landscape foxes and partridges had various structural elements at their disposal, which led to partial separation of the predator and its prey in the space.

Although Benzo[a]Pyrene (BaP) released to soils tends to bind very strongly to soil particles, however, small amounts have been shown to leach into groundwaters. This polycyclic aromatic hydrocarbon is stable and can stay (and travel) in the environment for an extended period of time. Release of BaP into the environment therefore causes concern at a global environmental level. In this paper we evaluated the effect of BaP on the cell growth of Escherichia coli and Enterococcus faecalis in aerobic conditions. Irradiation of BaP increased its antibacterial activity which suggests that this process produced a singlet oxygen. This way would be able to speed up the processes of photochemical degradation of BaP.

Phosphatidyl serine plays an important role in animal innate immunity. Given its important functions, numerous investigations have been carried out on its immunological function in many animals. However, studies of phosphatidyl serine in the white shrimp Litopenaeus vannamei, an economically important animal, are rare. In this paper, we demonstrated influences of injecting phosphatidyl serine (PS) on immune response including some parameters from pro-phenol oxidase activating system (pro-PO system) and hemocyanin-derived phenol oxidase activity (Hd-PO) along with antibacterial and bacteriolytic activities in the white shrimp Litopenaeus vannamei with different PS concentrations (5, 10 and 20 μg mL−1). The results showed that PS could affect immune response of L. vannamei significantly (P<0.05), including total hemocyte counts (THC), PO activity from hemocyte, phenol oxidase (PO) activity from plasma, hemocyanin concentration, Hd-PO activity as well as antibacterial and bacteriolytic activities in the plasma. Among the lines, 20 μg mL−1 PS had the strongest effect on the above parameters, whereas 5 μg mL−1 had the least effect. The experimental results indicated that PS was able to activate exocytosis of pro-PO and formation of Hd-PO in white shrimp after injection, further regulating the immune process reflected by variation of antibacterial and bacteriolytic activities in a certain way.

Temperature and precipitation are amongst the most important characteristics of local climatic conditions affecting the breeding biology of birds. Many bird species experiencing higher temperatures lay their eggs earlier and/or have bigger clutches. Higher precipitation can lead to a decrease in food availability and/or an increase of the costs of thermoregulation, which consequently may result in lower breeding success. In this paper we present differences in the breeding biology of two collared flycatcher (Ficedula albicollis) populations experiencing different temperatures and precipitation conditions associated with the different altitudes of their localities. We found differences in clutch size and nest success, as well as small differences in dates of egg laying. The population breeding at a higher altitude, and thus harsher conditions, was characterized by smaller clutches and lower nest success. In addition, this population was significantly affected by local weather, and showed correlation between both the beginning of egg laying and the local temperature, as well as between nest success and precipitation. Conversely, the population inhabiting the lower elevation locality with milder conditions showed no relationships with temperature or precipitation. These results suggest that severe conditions, such as high altitude, enhance the effect of local climatic conditions on collared flycatcher breeding ecology.