Identification of Two Chemotypes of Pogostemon cablin (Blanco) Benth. through DNA Barcodes
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Yang He
Pogostemon cablin (Blanco) Benth. is an important medicinal plant in Traditional Chinese Medicine (TCM). Because of differences in the chemical composition, this species has been classified into two major chemotypes, i. e. the patchouliol-type and the pogostone-type; however, no quick and effective method is presently available for the precise identification of these two chemotypes. DNA barcoding, using a standardized DNA fragment, is a promising molecular diagnostic method for species identification. We have established a reliable and quick method for the identification of the P. cablin chemotypes. Of five potential barcodes [rbcL, psbA-trnH, rpoB, ITS (internal transcibed spacer), and ndhJ], tested among 103 samples, ITS was the best candidate, as comparative studies between patchouliol-type and pogostone-type P. cablin revealed that ITS had more variable regions among these five barcodes. We suggest that ITS can serve as the most suitable barcode for differentiating between the chemotypes of P. cablin
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Articles in the same Issue
- Chemical Constituents of Croton oligandrum (Euphorbiaceae)
- Acacetin-7-rutinoside from Buddleja lindleyana, a New Molluscicidal Agent against Oncomelania hupensis
- Cytotoxic Saponins from the Seeds of Pittosporum angustifolium
- Flavone Composition and Antihypercholesterolemic and Antihyperglycemic Activities of Chrysanthemum coronarium L.
- New Nitrogenous Compounds from Anisotes trisulcus
- Inhibitory Effect of the Methanolic Extract of Verbascum latisepalum Hub.-Mor. on Endothelium-Dependent Relaxation in Rat Thoracic Aorta
- Exogenous Ascorbic Acid and Glutathione Alleviate Oxidative Stress Induced by Salt Stress in the Chloroplasts of Oryza sativa L.
- Improved ß-Carotene and Lycopene Production by Blakeslea trispora with Ultrasonic Treatment in Submerged Fermentation
- Microbial Metabolism of Danazol: A Contribution to Doping Analysis
- Identification of Two Chemotypes of Pogostemon cablin (Blanco) Benth. through DNA Barcodes
- cDNA Cloning and Expression Analysis of Farnesyl Pyrophosphate Synthase from Ornithogalum saundersiae
