Characterization on Biodegradation of Enzymatically Synthesized Polylactic Acid by Using Alkaline Protease and Lipase
-
D. Omay
Abstract
Biodegradation is the degradation process of organic substances via catabolic reactions that usually takes place in the presence of microorganisms and enzymes. In the present study, the enzymatic degradation of PLA was investigated by protease DSM and candida rugosa lipase enzymes. When the molecular weight loss in the degradation processes using protease DSM and candida rugosa lipase was examined, there was a 23 % reduction after 90 days for protease DSM, and 28 % reduction after 50 days for candida rugosa lipase. In addition, FT-IR, TGA, XRD and SEM analyses demonstrated significant changes in the characteristic, morphological and thermal structures of PLA during the enzymatic degradation processes.
References
Bone, R., Shenvi, A. R., Kettner, C. A. and Agard, D. A., “Serine Protease Mechanism: The Structure of an Inhibitory Complex of Alpha-lytic Protease and a Tightly Bound Peptide Boronic Acid”, Biochem., 26, 7609–7614 (1987) 10.1021/bi00398a012Suche in Google Scholar
Gautam, R., Bassi, A. S., Yanful, A. and Cullen, E., “Biodegradation of Automotive Waste Polyester Polyurethane Foam Using Pseudomonas Chlororaphis ATCC55729”, Int. Biodeterior. Biodegrad., 60, 245–249 (2007) 10.1016/j.ibiod.2007.03.009Suche in Google Scholar
Hedstrom, L., “Serine Protease Mechanism and Specificity”, Chem. Rev., 102, 4501–4524 (2002) 10.1021/cr000033xSuche in Google Scholar
Kale, G., Auras, R. and Singh, S. P., “Comparison of the Degradability of Poly(lactide) Packages in Composting and Ambient Exposure Conditions”, Pack. Technol. Sci., 20, 49–70 (2007)10.1002/pts.742Suche in Google Scholar
Kunioka, M., Ninomiya, F. and Funabashi, M., “Biodegradation of Poly(lactic acid) Powders Proposed as the Reference Test Materials for the International Standard of Biodegradation Evaluation Methods”, J. Polym. Environ., 15, 7–17 (2006)Suche in Google Scholar
Li, S., Liu, L., Garreau, G. and Vert, M., “Lipase Catalyzed Biodegradation of Poly(e-caprolactone) Blended with Various Polylactide Based Polymers”, Biomacromolecules, 4, 372–377 (2003) 10.1021/bm025748jSuche in Google Scholar
Masaki, K., Kamini, N. R., Ikeda, H. and Lefuji, H., “Cutinase-Like Enzyme from the Yeast Cryptococcus SP. Strain S-2 Hydrolyzes Polylactic Acid and Other Biodegradable Plastics”, Appl. Environ. Microbiol., 71, 7548–7550 (2005) 10.1128/AEM.71.11.7548-7550.2005Suche in Google Scholar
Omay, D., Güvenilir, Y., “Synthesis and Characterization of Polylactic Acid via Enzymatic Ring Opening Polymerization by Using Free and Immobilized Lipase”, Biocatal. Biotransform., 31, 132–140, (2013)10.3109/10242422.2013.795148Suche in Google Scholar
Paradis, E., Clement, S., Julien, P. and Ven Murty, M. R., “Lipoprotein Lipase Affects the Survival and Differentiation of Neural Cells Exposed to Very Low Density Lipoprotein”, J. Biol. Chem., 278, 9698–9705 (2002) 10.1074/jbc.M208452200Suche in Google Scholar
Sukkhum, S., Tokuyama, S. and Kitpreechavanich, V., “Poly(L-lactide)-Degrading Enzyme Production by Actinomadura Keratinilytica T16-1 in 3 L Airlift Bioreactor and Its Degradation Ability for Biological Recycle”, J. Microbiol. Biotechnol., 22, 92–99 (2012) 10.4014/jmb.1105.05016Suche in Google Scholar
Svendsen, A., “Lipase Protein Engineering”, Biochim. Biophys. Acta, 1543, 223–228 (2000)10.1016/S0167-4838(00)00239-9Suche in Google Scholar
Vichaibun, V., Chulavatnatol, M., “A New Assay for the Enzymatic Degradation of Polylactic Acid”, Sci. Asia, 29, 297–300 (2003) 10.2306/scienceasia1513-1874.2003.29.297Suche in Google Scholar
Yuan, X., Mak, A. F. T. and Yao, K., “Comparative Observation of Accelerated Degradation of Poly(L-lactic acid) Fibres in Phosphate Buffered Saline and a Dilute Alkaline Solution”, Polym. Degrad. Stab., 75. 1, 45–53 (2002) 10.1016/S0141-3910(01)00203-8Suche in Google Scholar
Xu, J., Zhang, J., Gao, W., Liang, H., Wang, H. and Li, J., “Preparation of Chitosan/PLA Blend Micro/Nanofibers by Electrospinning”, Mater. Lett., 63, 658–660 (2009) 10.1016/j.matlet.2008.12.014Suche in Google Scholar
Walter, T., Augusta, J., Muller, R. J., Widdecke, H. and Klein, J., “Enzymatic Degradation of a Model Polyester by Lipase from Rhizopus Delemar”, Enzyme Microb. Technol., 17, 218–224 (1995) 10.1016/0141-0229(94)00007-ESuche in Google Scholar
Wang, C., Fan, K. and Hsiue, G., “Enzymatic Degradation of PLLA-PEOZ-PLLA Triblock Copolymers”, Biomaterial., 26, 2803–2811 (2005) 10.1016/j.biomaterials.2004.07.064Suche in Google Scholar PubMed
Winkler, F. K., D’Arcy, A., Hunziker, W., “Structure of Human Pancreatic Lipase”, Nature, 343, 771–774 (1990) 10.1038/343771a0Suche in Google Scholar PubMed
© 2014, Carl Hanser Verlag, Munich
Artikel in diesem Heft
- Contents
- Contents
- Regular Contributed Articles
- The Processing Behavior of Liquid Sn/Molten Polyethylene during Internal Mixing
- Study on Packing Phase Control Based on the Cavity Pressure-Temperature during Injection Molding
- Synthesis and Properties of a Novel Intumescent Flame Retardant and its Application in LLDPE
- The Mechanism of Neck-in Phenomenon in Film Casting Process
- Morphology, Mechanical and Thermal Properties of Recycled PC/ABS Blends Processed via Vane Extruder
- Thermal Conductivity of Polypropylene Composites with Combined Boron Nitride Fillers
- Characterization on Biodegradation of Enzymatically Synthesized Polylactic Acid by Using Alkaline Protease and Lipase
- Numerical Investigation and Experimental Observation of Extrudate Swell for Viscoelastic Polymer Melts
- Processing and Wear Analysis of Blast Furnace Slag Filled Polypropylene Composites Using Taguchi Model and ANN
- Microchannel Deformation of Polymer Chip in In-Mold Bonding
- Synthesis and Characteristics of Graft Copolymerization of Starch-G-PAM Using A Twin-Roll Mixer as Reactor for Cornstarch with Different Amylose/Amylopectin Ratios
- Experimental Investigation of PC-Films Using Optical Measurements
- Effect of Compounding Conditions on Static and Dynamic Mechanical Properties of High Density Polyethylene/Ground Tire Rubber Blends
- Effect of Feeding Strategy on the Mechanical Properties of PP/Recycled EPDM/PP-G-MA Blends
- Synthesis and Characterization of Chitosan & Amino Acid Superabsorbent Hydrogels
- Rapid Communications
- Negative Pressures in Modelling Rotating Polymer Processing Machinery Are Meaningless, But They Are Telling Something
- PPS News
- PPS News
- Seikei-Kakou Abstracts
- Seikei-Kakou Abstracts
Artikel in diesem Heft
- Contents
- Contents
- Regular Contributed Articles
- The Processing Behavior of Liquid Sn/Molten Polyethylene during Internal Mixing
- Study on Packing Phase Control Based on the Cavity Pressure-Temperature during Injection Molding
- Synthesis and Properties of a Novel Intumescent Flame Retardant and its Application in LLDPE
- The Mechanism of Neck-in Phenomenon in Film Casting Process
- Morphology, Mechanical and Thermal Properties of Recycled PC/ABS Blends Processed via Vane Extruder
- Thermal Conductivity of Polypropylene Composites with Combined Boron Nitride Fillers
- Characterization on Biodegradation of Enzymatically Synthesized Polylactic Acid by Using Alkaline Protease and Lipase
- Numerical Investigation and Experimental Observation of Extrudate Swell for Viscoelastic Polymer Melts
- Processing and Wear Analysis of Blast Furnace Slag Filled Polypropylene Composites Using Taguchi Model and ANN
- Microchannel Deformation of Polymer Chip in In-Mold Bonding
- Synthesis and Characteristics of Graft Copolymerization of Starch-G-PAM Using A Twin-Roll Mixer as Reactor for Cornstarch with Different Amylose/Amylopectin Ratios
- Experimental Investigation of PC-Films Using Optical Measurements
- Effect of Compounding Conditions on Static and Dynamic Mechanical Properties of High Density Polyethylene/Ground Tire Rubber Blends
- Effect of Feeding Strategy on the Mechanical Properties of PP/Recycled EPDM/PP-G-MA Blends
- Synthesis and Characterization of Chitosan & Amino Acid Superabsorbent Hydrogels
- Rapid Communications
- Negative Pressures in Modelling Rotating Polymer Processing Machinery Are Meaningless, But They Are Telling Something
- PPS News
- PPS News
- Seikei-Kakou Abstracts
- Seikei-Kakou Abstracts