Fabrication of Porous 3-D Structure from Poly(L-lactide)-based Nanocomposite Foam via Enzymatic Degradation
-
M. Bitou
and M. Okamoto
Abstract
In order to prepare a porous three-dimensional (3-D) structure in biodegradable polyester materials we have conducted the enzymatic degradation of a poly(L-lactide) (PLLA)-based nano-composite foam, having a nanocellular structure, using proteinase-K as a degrading agent at 37Β°C. The surface and cross sectional morphologies of the foam recovered after enzymatic hydrolysis for different intervals were investigated by using a scanning electron microscope. The nanocellular material took up a large amount of water, which led to the swelling of the foam due to the large surface area inside the nanocelluar structure, and facilitated the enzymatic degradation of the PLLA matrix as compared with the bulk (pre-foamed) sample. Consequently, we have successfully prepared a porous 3-D structure as a remaining scaffold in the core part of the nano-composite foam, reflecting the spherulites of the crystallized PLLA.
References
Abe, H., et al., βPhysical Properties and Enzymatic Degradability of Copolymers of (R)-3-Hydroxybutyric Acid and (S, S) Lactideβ, Polymer, 39, 59β67 (1997)10.1016/S0032-3861(97)00240-1Search in Google Scholar
Cai, Q., et al., βEnzymatic Degradation Behavior and Mechanism of Poly(Lactide-Co-Glycolide) Foams by Trypsinβ, Biomaterials, 24, 629β638 (2003)10.1016/S0142-9612(02)00377-0Search in Google Scholar
Coombes, A. G. A., Meikle, M. C., βResorbable Synthetic Polymers as Replacements for Bone Graftβ, Mater.17, 35 (1994)10.1016/0267-6605(94)90046-9Search in Google Scholar
Ebeling, W.et al., βProteinase K from Tritirachium Album Limberβ, Eur. J. Biochem., 47, 91β97 (1974)10.1111/j.1432-1033.1974.tb03671.xSearch in Google Scholar
Ema, Y., et al., βFoam Processing and Cellular Structure of Polylactide-based Nanocompositesβ, Polymer, 47, 5350β5359 (2006)10.1016/j.polymer.2006.05.050Search in Google Scholar
Fisher, E. W., et al., βInvestigation of the Structure of Solution Grown Crystals of Lactide Copolymers by Means of Chemical Reactionsβ, Polymer, 25, 980β990 (1973)Search in Google Scholar
Fujimoto, Y., et al., βWell-controlled Biodegradable Nanocomposite Foams: From Microcellular to Nanocellularβ, Macromol. Rapid Commun., 24, 457β461 (2003)10.1002/marc.200390068Search in Google Scholar
Fukuda, N., et al., βPhysical Properties and Enzymatic Hydrolysis of Poly (L-lactide)-CaCO3 Compositesβ, Polym. Degrad. Stab., 78, 119β127 (2002)10.1016/S0141-3910(02)00125-8Search in Google Scholar
Gao, F., βClay/Polymer Composites: The Storyβ, Materials Today, 7, 50β55 (2004)10.1016/S1369-7021(04)00509-7Search in Google Scholar
Gedde, U. W., βPolymer Physicsβ, Chapman & Hall, London (1995)Search in Google Scholar
Hoedle, S. M., et al., βSupercritical Fluid Mixing: Preparation of Thermally Sensitive Polymer Composites Containing Bioactive Materialsβ, Chem. Commun. 109β110 (2001)10.1039/b008188oSearch in Google Scholar
Hussain, F., et al., βReview Paper: Polymer-Matrix Nanocomposites, Processing, Manufacturing, and Application: An Overviewβ, J. Composite Mater., 40, 1511β1575 (2006)10.1177/0021998306067321Search in Google Scholar
Iwata, T., Doi, Y., βMorphology and Enzymatic Degradation of Poly-(L-lactic acid) Single Crystalsβ, Macromolecules31, 2461β2467 (1998)10.1021/ma980008hSearch in Google Scholar
Li, S., et al., βEnzymatic Degradation of Polylactide Stereocopolymers with Predominant d-lactyl Contentsβ, Polym. Degrad. Stab., 71, 61β67 (2001)10.1016/S0141-3910(00)00152-XSearch in Google Scholar
Nam, H., et al., βA Hierarchical Structure and Properties of Intercalated Polypropylene/Clay Nanocompositesβ, Polymer, 42, 9633β9640 (2001)10.1016/S0032-3861(01)00512-2Search in Google Scholar
Nam, J., et al., βCrystallization Behavior and Morphology of Biodegradable Polylactide/Layered Silicate Nanocompositeβ, Macromolecules, 36, 7126β7131 (2003)10.1021/ma034623jSearch in Google Scholar
Okada, A,. UsukiA., βTwenty Years of Polymer-Clay Nanocompositesβ, Macromol. Mater. Eng., 291, 1449β1476 (2006)10.1002/mame.200600260Search in Google Scholar
Okamoto, M., βRecent Advances in Polymer/Layered Silicate Nanocomposites: An Overview from Science to Technologyβ, Mater. Sci. Tech., 22, 756β779 (2006)10.1179/174328406X101319Search in Google Scholar
Reeve, M. S., et al., βPolyactide Stereochemistry: Effect on Enzymatic Degradabilityβ, Macromolecules, 27, 825 (1994)10.1021/ma00081a030Search in Google Scholar
Sinha Ray, S., et al., βNew Polylactide/Layered Silicate Nanocomposites. 1. Preparation, Characterization, and Propertiesβ, Macromolecules, 35, 3104β3110 (2002a)10.1021/ma011613eSearch in Google Scholar
Sinha Ray, S., et al., βNew Polylactide/Layered Silicate Nanocomposite: Nanoscale Control over Multiple Propertiesβ, Macromol. Rapid. Commun., 23, 943β947 (2002b)10.1002/1521-3927(200211)23:16<943::AID-MARC943>3.0.CO;2-FSearch in Google Scholar
Sinha Ray, S., et al., βPolylactide/Layered Silicate Nanocomposite: A Novel Biodegradable Materialβ, Nano Lett., 2, 1093β1096 (2002c)10.1021/nl0202152Search in Google Scholar
Sinha Ray, S., Okamoto, M., βPolymer/Layered Silicate Nanocomposites: A Review from Preparation to Processingβ, Prog. Polym. Sci., 28, 1539β1641 (2003a)10.1016/j.progpolymsci.2003.08.002Search in Google Scholar
Sinha Ray, S., et al., βNew Polylactide-layered Silicate Nanocomposites. 2. Concurrent Improvements of Material Properties, Biodegradability and Melt Rheologyβ, Polymer, 44, 857β866 (2003b)10.1016/S0032-3861(02)00818-2Search in Google Scholar
Sinha Ray, S., et al., βNew Polylactide/Layered Silicate Nanocomposites. 5. Designing of Materials with Desired Propertiesβ, Polymer, 44, 6633β6646 (2003c)10.1016/j.polymer.2003.08.021Search in Google Scholar
Sinha Ray, S., et al., βNew Polylactide/Layered Silicate Nanocomposites. 3. High-Performance Biodegradable Materialsβ, Chem. Mater., 15, 1456β1465 (2003d)10.1021/cm020953rSearch in Google Scholar
Sinha Ray, S., et al., βControl of Biodegradability of Polylactide via Nanocomposite Technologyβ, Macromol. Mater. Eng., 288, 203β208 (2003e)10.1002/mame.200300156Search in Google Scholar
Sinha Ray, S., et al., βNew Polylactide/Layered Silicate Nanocomposites, 6. a. Melt Rheology and Foam Processingβ, Macromol. Mater. Eng., 288, 936β944 (2003f)10.1002/mame.200300156Search in Google Scholar
Sinha Ray, S., Okamoto, βBiodegradable Polylactide and its Nanocomposites: Opening a New Dimension for Plastics and Compositesβ, Macromol. Rapid. Commun., 24, 815β840 (2003g)10.1002/marc.200300008Search in Google Scholar
Tsuji, H., et al., J. βPorous Biodegradable Polyesters. I. Preparation of Porous Poly (L-Iactide) Films by Extraction of Poly(ethylene oxide) from Their Blendsβ, J. Appl. Polm. Sci., 75, 629β637 (2000)10.1002/(SICI)1097-4628(20000131)75:5<629::AID-APP5>3.0.CO;2-ASearch in Google Scholar
Tsuji, H., Ishizaka, T., βPorous Biodegradable Polyesters: 3. Preparation of Porous Poly(β-caprolactone) Films from Blends by Selective Enzymatic Removal of Poly(L-lactide)β, Macromol. Biosci., 1, 59β65 (2001)10.1002/1616-5195(20010301)1:2<59::AID-MABI59>3.0.CO;2-6Search in Google Scholar
Tsuji, H., Miyauchi, S., βPoly(l-lactide): VI Effects of Crystallinity on Enzymatic Hydrolysis of Poly(l-lactide) without Free Amorphous Regionβ, Polym. Degrad. Stab., 71, 415β424 (2001)10.1016/S0141-3910(00)00191-9Search in Google Scholar
Vaia, R. A., Wagner, H. D., βFramework for Nanocompositesβ, Materials Today, 7, 32β37 (2004)10.1016/S1369-7021(04)00506-1Search in Google Scholar
Wang, C. H., et al., βEnzymatic Degradation of PLLA-Peoz-PLLA Triblock Copolymersβ, Biomaterials, 26, 2803β2811 (2005)10.1016/j.biomaterials.2004.07.064Search in Google Scholar PubMed
Williams, D. F., βEnzymic Hydrolysis of Polylactic Acidβ, Eng. Med., 10, 5β7 (1981)10.1243/EMED_JOUR_1981_010_004_02Search in Google Scholar
Yoshida, O., Okamoto, M., βDirect Melt Intercalation of Polylactide Chains into Nano-Galleries: Interlayer Expansion and Nanocomposite Structureβ, Macromol. Rapid Commun., 27, 751β757 (2006)10.1002/marc.200600080Search in Google Scholar
Zhang, J., et al., βCrystal Modifications and Thermal Behavior of Poly(L-lactic acid) Revealed by Infrared Spectroscopyβ, Macromolecules, 38, 8012β8021 (2005)10.1021/ma051232rSearch in Google Scholar
Β© 2007, Carl Hanser Verlag, Munich
Articles in the same Issue
- Contents
- Contents
- Editorial
- Special Issue on Biobased Polymers
- Invited Papers
- Crystallization and Mechanical Propertiesof Poly (D, L) Lactide-based Blown Films
- Rheological Behavior and Modeling of Thermal Degradation of Poly(β-Caprolactone) and Poly(L-Lactide)
- Rheological Evaluation and Observations of Extrusion Instabilities of Biodegradable Polyesters
- Biaxial Orientation of Polylactide/Thermoplastic Starch Blends
- Effects of Starch Types on Mechanical Properties of Poly(lactic acid)/Starch Composites
- Solid and Microcellular Polylactide-Carbon Nanotube Nanocomposites
- Tapioca Starch-poly (lactic acid)-based Nanocomposite Foams as Affected by Type of Nanoclay
- Injection Molded Solid and Microcellular Polylactide Compounded with Recycled Paper Shopping Bag Fibers
- Fabrication of Porous 3-D Structure from Poly(L-lactide)-based Nanocomposite Foam via Enzymatic Degradation
- The Linear Viscoelastic Behavior of a Series of 3-Hydroxybutyrate-based Copolymers
- New Developments in Biodegradable Starch-based Nanocomposites
- Viscous Properties of Thermoplastic Starches from Different Botanical Origin
- Thermoplastic Foams from Zein and Gelatin
- Improvement of the Mechanical Properties of Soy Protein Isolate Based Plastics through Formulation and Processing
- Biocomposites Based on Bacterial Cellulose and Apple and Radish Pulp
- Preparation and Properties of Metallocene-catalyzed PE/Starch Nanocomposites: Role of Nanocompatibilizer
- Evaluation of Properties and Biodeterioration Potential of Polyethylene and Aliphatic Polyester Blends
- PPS News
- PPP News
- Seikei-Kakou Abstracts
- Seikei-Kakou Abstracts
Articles in the same Issue
- Contents
- Contents
- Editorial
- Special Issue on Biobased Polymers
- Invited Papers
- Crystallization and Mechanical Propertiesof Poly (D, L) Lactide-based Blown Films
- Rheological Behavior and Modeling of Thermal Degradation of Poly(β-Caprolactone) and Poly(L-Lactide)
- Rheological Evaluation and Observations of Extrusion Instabilities of Biodegradable Polyesters
- Biaxial Orientation of Polylactide/Thermoplastic Starch Blends
- Effects of Starch Types on Mechanical Properties of Poly(lactic acid)/Starch Composites
- Solid and Microcellular Polylactide-Carbon Nanotube Nanocomposites
- Tapioca Starch-poly (lactic acid)-based Nanocomposite Foams as Affected by Type of Nanoclay
- Injection Molded Solid and Microcellular Polylactide Compounded with Recycled Paper Shopping Bag Fibers
- Fabrication of Porous 3-D Structure from Poly(L-lactide)-based Nanocomposite Foam via Enzymatic Degradation
- The Linear Viscoelastic Behavior of a Series of 3-Hydroxybutyrate-based Copolymers
- New Developments in Biodegradable Starch-based Nanocomposites
- Viscous Properties of Thermoplastic Starches from Different Botanical Origin
- Thermoplastic Foams from Zein and Gelatin
- Improvement of the Mechanical Properties of Soy Protein Isolate Based Plastics through Formulation and Processing
- Biocomposites Based on Bacterial Cellulose and Apple and Radish Pulp
- Preparation and Properties of Metallocene-catalyzed PE/Starch Nanocomposites: Role of Nanocompatibilizer
- Evaluation of Properties and Biodeterioration Potential of Polyethylene and Aliphatic Polyester Blends
- PPS News
- PPP News
- Seikei-Kakou Abstracts
- Seikei-Kakou Abstracts