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Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method

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Published/Copyright: May 9, 2016
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Tenside Surfactants Detergents
From the journal Tenside Surfactants Detergents Volume 53 Issue 3

Abstract

A rapid and reliable method to quantify rhamnolipid is indispensable to study and evaluate rhamnolipid-producing bacteria. Five methods were attempted to quantify rhamnolipid in bacteria culture. The oil spreading method better predicted the rhamnolipid concentration and is simpler than the other methods. The potential influencing factors (temperature, pH, salinity, metals, bacterial cells, carbon sources) were investigated. The common substance in fermentation broth has little effect on oil spreading circle. An optimized protocol of the oil spreading method to quantify rhamnolipid was proposed. A positive linear correlation (R2 – 0.9908) was established between the diameter of the formed oil spreading circle and the concentration of rhamnolipid (100–800 mg L−1). Results of a large number of samples suggested that the oil spreading method is easy, rapid and reliable to analyze quantitatively rhamnolipids.

Kurzfassung

Für die Untersuchung und Bewertung der Rhamnolipid-erzeugenden Bakterien ist eine schnelle und zuverlässige Methode zur Quantifizierung von Rhamnolipid unerlässlich. Es wurden fünf Methoden eingesetzt, um Rhamnolipid in einer Bakterienkultur zu quantifizieren. Unter diesen fünf Methoden erwies sich die Ölspreitungsmethode bei der Vorhersage der Rhamnolipid-Konzentration als die beste und einfachste. Mögliche Einflussfaktoren (Temperatur, pH-Wert, Salzgehalt, Metalle, Bakterienzellen, Kohlenstoffquellen) wurden untersucht. Bei der Fermantation übliche Substanzen hatten auf den Kreis der Ölspreitung kaum Einfluss. Eine positive lineare Korrelation von R2 = 0.9908 konnte zwischen dem Durchmesser des Ölspreitungskreises und der Rhamnolipid-Konzentration (100–800 mg L−1) festgestellt warden. Die Ergebnisse für eine große Anzahl an Proben zeigen, dass die Ölspreitungsmethode einfach, schnell und zuverlässig ist, das Rhamnolipid quantitativ zu analysieren.

Key words: Rhamnolipid; quantification; oil spreading method; optimization
Stichwörter: Rhamnolipid; Quantifizierung; Ölspreitungsmethode; Optimierung
*Correspondence address Professor Fang Ma, State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Tel.: +8645186282107, E-Mail address:

F. Zhao, PhD student, State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, China. At present he is studying for biosurfactants and MEOR.

X. Liang, graduate student, Institute of Applied Ecology, Chinese Academy of Sciences. At present he is studying lipopeptides-producing strain.

Y. Ban, graduate student, College of Chemistry and Life Science, Shenyang Normal University. He is currently involved in lipopeptides.

S. Han, assistant researcher, Institute of Applied Ecology, Chinese Academy of Sciences. Her research interest includes biosurfactants, fermentation, microbiology etc.

J. Zhang, Professor, State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology. For the past twenty years he is involved in the teaching, research and development in the field of water treatment, biotechnology, biotransformation etc.

Y. Zhang, Researcher, Institute of Applied Ecology, Chinese Academy of Sciences. For the past twenty five years she is involved in the teaching, research and development in the field of biosurfactants, fermentation, biotechnology etc.

F. Ma, Professor, State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, China. For the past twenty years he is involved in the teaching, research and development in the field of biosurfactants, fermentation, water treatment, biotechnology, biotransformation etc.

References

1. Lang, S.: Biological amphiphiles (microbial biosurfactant). Curr. Opin. Colloid Inter. Sci.7 (2002) 12–20. 10.1016/S1359-0294(02)00007-9Search in Google Scholar

2. Soberón-Chávez, G., Lepine, F. and Déziel, E.: Production of rhamnolipids by Pseudomonas aeruginosa. Appl. Microbiol. Biotechnol.68 (2005) 718–725. 10.1007/s00253-005-0150-3Search in Google Scholar PubMed

3. Płociniczak, M. P., Płaza, G. A., Piotrowska-Seget, G. and Cameotra, S. S.: Environmental Applications of Biosurfactant: Recent Advances. Int. J. Mol. Sci.12 (2011) 633–654. 10.3390/ijms12010633Search in Google Scholar PubMed PubMed Central

4. Nitschke, M., Costa, S. G. and Contiero, J.: Rhamnolipid surfactants: an update on the general aspects of these remarkable biomolecules. Biotechnol. Prog.21 (2005) 1593–1600. 10.1021/bp050239pSearch in Google Scholar PubMed

5. Müller, M. M., Kügler, J. H., Henkel, M., Gerlitzki, M., Hörmann, B., Pöhnlein, M., Syldatk, C. and Hausmann, R.: Rhamnolipids-next generation surfactants?J. Biotechnol. 162 (2012) 366–380. 10.1016/j.jbiotec.2012.05.022Search in Google Scholar PubMed

6. Sen, R.: Biotechnology in petroleum recovery: the microbial EOR. Prog Energy Combust Sci34 (2008) 714–724. 10.1016/j.pecs.2008.05.001Search in Google Scholar

7. Brown, L. R.: Microbial enhanced oil recovery (MEOR). Curr. Opin. Microbiol.13 (2010) 316–320. 10.1016/j.mib.2010.01.011Search in Google Scholar PubMed

8. Amani, H., Müller, M. M., Syldatk, C. and Hausmann, R.: Production of microbial rhamnolipid by Pseudomonas Aeruginosa MM1011 for ex situ enhanced oil recovery. Appl. Biochem. Biotechnol.170 (2013) 1080–1093. 10.1007/s12010-013-0249-4Search in Google Scholar PubMed

9. Dean, S. M., Jin, Y., Cha, D. K., Wilson, S. V. and Radosevich, M.: Phenanthrene degradation in soils co-inoculated with phenanthrene-degrading and biosurfactant-producing bacteria. J. Environ. Qual.30 (2001) 1126–1133. 10.2134/jeq2001.3041126xSearch in Google Scholar PubMed

10. Mulligan, C. N.: Environmental applications for biosurfactants. Environ. Pollut.133 (2005) 183–198. 10.1016/j.envpol.2004.06.009Search in Google Scholar PubMed

11. Whang, L. M., Liu, P. W. G., Ma, C. C. and Cheng, S. S.: Application of biosurfactant, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil. J. Hazard. Mater.151 (2008) 155–163. 10.1016/j.jhazmat.2007.05.063Search in Google Scholar PubMed

12. Dubois, M., Gills, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F.: Colorimetric method for determination of sugar and related substances. Anal Chem28 (1956) 350–356. 10.1021/ac60111a017Search in Google Scholar

13. Al-Tahhan, R. A., Sandrin, T. R., Bodour, A. A. and Maier, R. M.: Rhamnolipid-induced removal of lipopolysaccharide from Pseudomonas aeruginosa: Effect on cell surface properties and interaction with hydrophobic substrates. Appl. Environ. Microbiol.66 (2010) 3262–3268. 10.1128/AEM.66.8.3262-3268.2000Search in Google Scholar PubMed PubMed Central

14. Cha, M., Lee, N., Kim, M.J., Kim, M. and Lee, S. J.: Heterologous production of Pseudomonas aeruginosa EMS1 biosurfactants in Pseudomonas putida. Bioresour. Technol.99 (2008) 2192–2199. 10.1016/j.biortech.2007.05.035Search in Google Scholar PubMed

15. Wang, Q.H., Fang, X., Bai, B., Liang, X., Shuler, P.J., GoddardIII, W. A. and Tang, Y.: Engineering bacteria for production of rhamnolipid as an agent for enhanced oil recovery. Biotechnol. Bioeng.98 (2007) 842–853. 10.1002/bit.21462Search in Google Scholar PubMed

16. Bharali, P., Singh, S. P., Dutta, N., Gogoi, S., Bora, L. C., Debnath, P. and Konwar, B. K.: Biodiesel derived waste glycerol as an economic substrate for biosurfactant production using indigenous Pseudomonas aeruginosa. RSC Adv4 (2014) 38698–38706. 10.1039/C4RA05594BSearch in Google Scholar

17. Yin, H., Qiang, J., Jia, Y., Ye, J. S., Peng, H., Qin, H. M., Zhang, N. and He, B. Y.: Characteristics of biosurfactants produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater. Process Biochem44 (2009) 302–308. 10.1016/j.procbio.2008.11.003Search in Google Scholar

18. Zhao, F., Mandlaa, M., Hao, J., Liang, X., Shi, R., Han, S. and Zhang, Y.: Optimization of culture medium for anaerobic production of rhamnolipid by recombinant Pseudomonas stutzeri Rhl for microbial enhanced oil recovery. Lett. Appl. Microbiol.59 (2014) 231–237. 10.1111/lam.12269Search in Google Scholar PubMed

19. Zhang, Y. M. and Miller, R. M.: Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl. Environ. Microbiol.58 (1992) 3276–3282. 0099-2240/92/103276-07$02.00/0Search in Google Scholar

20. Zhao, F., Zhang, J., Shi, R., Han, S., Ma, F. and Zhang, Y.: Production of biosurfactant by a Pseudomonas aeruginosa isolate and its applicability in in situ microbial enhanced oil recovery under anoxic conditions. RSC Adv5 (2015) 36044–36050. 10.1039/C5RA03559GSearch in Google Scholar

21. Xia, W. J., Luo, Z. B., Dong, H. P., Yu, L., Cui, Q. F. and Bi, Y. Q.: Synthesis, Characterization, and Oil Recovery Application of Biosurfactants Produced by Indigenous Pseudomonas aeruginosa WJ-1 Using Waste Vegetable Oils. Appl. Biochem. Biotechnol.166 (2012) 1148–1166. 10.1007/s12010-011-9501-ySearch in Google Scholar PubMed

22. Zendehboudi, S., Chatzis, I., Mohsenipour, A. A., Elkamel, A.: Dimensional Analysis and Scale-up of Immiscible Two-Phase Flow Displacement in Fractured Porous Media under Controlled Gravity Drainage. Energy Fuels25 (2011) 1731–1750. 10.1021/ef101506nSearch in Google Scholar

23. Shafiei, A., Dusseault, M. B., Zendehboudi, S. and Chatzis, I.: A new screening tool for evaluation of steamflooding performance in Naturally Fractured Carbonate Reservoirs. Fuel108 (2013) 502–514. 10.1016/j.fuel.2013.01.056Search in Google Scholar

24. Herman, D. C., Artiola, J. F. and Miller, R.M.: Removal of cadmium, lead, and zinc from soil by a rhamnolipid biosurfactant. Environ. Sci. Technol.29 (1995) 2280–2285. 10.1021/es00009a019Search in Google Scholar PubMed

25. Youssef, N. H., Duncan, K. E., Nagle, D. P., Savage, K. N., Knapp, R. M. and McInerney, M. J.: Comparison of methods to detect biosurfactant production by diverse microorganisms. J. Microbiol. Methods.56 (2004) 339–347. 10.1016/j.mimet.2003.11.001Search in Google Scholar PubMed

26. Morikawa, M., Hirata, Y. and Imanaka, T.: A study on the structure – function relationship of the lipopeptide biosurfactants. Biochim Biophys Acta1488 (2000) 211–218. 10.1016/S1388-1981(00)00124-4Search in Google Scholar

Received: 2015-02-09
Accepted: 2015-11-09
Published Online: 2016-05-09
Published in Print: 2016-05-17

© 2016, Carl Hanser Publisher, Munich

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https://doi.org/10.3139/113.110429
Keywords for this article
Rhamnolipid; quantification; oil spreading method; optimization

Articles in the same Issue

  1. Contents/Inhalt
  2. Contents
  3. Application
  4. Feasibility of a Natural Surfactant as a Stabilizer for Cosmetics with Liposome-Encapsulated Plant Stem Cells: Pre-Formulation and Formulation Through Stability Studies
  5. Cleaning Technology
  6. Large Washing Machines Are Not Used Efficiently in Europe
  7. Environmental Chemistry
  8. Carboxylate-Terminated Double-Hydrophilic Block Copolymer as an Effective Inhibitor for Carbonate and Sulphate Scales
  9. Novel Surfactants
  10. Comparison of Methods to Quantify Rhamnolipid and Optimization of Oil Spreading Method
  11. Synthesis and Characterization of Biodegradable Cationic Esterquat Surfactants and the Evaluation of its Physico-Chemical Properties
  12. Characteristics of Block Copolymers of Methyl Oxirane and Oxirane Derivatives of 2-Ethylhexanol as Obtained with KOH and Dimetalcyanide Type Catalyst
  13. Physical Chemistry
  14. Amphiphile/Water/Decanol Lyotropic Liquid Crystalline System: Study of Thermal States of Anisometric Micelles in Nematic-Calamitic and Nematic-Discotic Mesophases
  15. Transition from Micelle to Vesicle of a Novel Sugar-Based Surfactant Containing Trisiloxane
  16. Synthesis
  17. One-Pot Preparation of Nano-SiO2 Using a Silane Derivative as a Coupling Agent
  18. Green Synthesis and Surface Properties of Acyl Glycine Surfactants Derived from Vegetable Oils
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