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Liquid Chromatography with Fluores-cence Detection as a Tool for Separation of Endocrine Disrupting Alkylphenols and their Mono- and Diethoxylates in Analysis of River Water Samples

  • A. Zgoła-Grześkowiak and T. Grześkowiak
Published/Copyright: April 2, 2013
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Tenside Surfactants Detergents
From the journal Tenside Surfactants Detergents Volume 46 Issue 4

Abstract

A simple, accurate and reproducible reversed-phase high performance liquid chromatography method with fluorescence detection was developed for determination of octylphenol, nonylphenol and their short-chain ethoxylates, using an octadecyl silica column. The mobile phase used for the analysis consisted of methanol, acetonitrile and water (50:15:35). The analytes were separated successfully within a reasonable time without gradient elution, which was not achieved previously. Thus, use of inexpensive fluorescence detection instead of MS detection was enabled. This chromatographic method, combined with solid-phase extraction, was highly applicable for the simultaneous determination of octyl- and nonylphenol as well as their mono- and diethoxylates in real river water samples. The limit of detection (LOD) for octylphenol and its ethoxylates was 0.05 μg · L−1. For nonylphenol the LOD was 0.14 μg · L−1 and for its ethoxylates it was 0.15 μg · L−1. Recovery of octylphenol and its ethoxylates was about 80%. Recovery of nonylphenol and its ethoxylates was slightly lower (from 60 to 76%).

Kurzfassung

Eine einfache, genaue und reproduzierbare Umkehrphasen-Hochdruckflüssigkeitschromatographie mit Fluoreszenzdetektion wurde unter Verwendung einer Octadecyl-Silica-Säule für die Bestimmung von Octylphenol, Nonylphenol und ihrer kurzkettigen Ethoxylate entwickelt. Die für die Analyse verwendete mobile Phase bestand aus Methanol, Acetonitril und Wasser (50:15:35). Die Analyten wurden in einer angemessenen Zeit ohne Gradient-Elution erfolgreich getrennt, was bisher nicht erreicht wurde. Somit wurde eine kostengünstige Fluoreszenzdetektion anstatt einer MS Detektion ermöglicht. Diese Chromatographiemethode, kombiniert mit einer Festphasenextraktion, war in hohem Maße für die gleichzeitige Bestimmung von Octylphenol und Nonylphenol sowie für ihre Mono- and Diethoxylate in realen Flusswasserproben anwendbar. Die Nachweisgrenze (NWG) für Octylphenol und seiner Ethoxylate lag bei 0,05 μg · L−1 und für Nonylphenol und seiner Ethoxylate bei 0,15 μg · L−1. Die Wiederfindung von Octylphenol und seiner Ethoxylate lag bei ca. 80% und die von Nonylphenol und seiner Ethoxylate (von 60 bis 76%) etwas niedriger.

Key words:: Alkylphenol; alkylphenol ethoxylates; river water; fluorescence detection
Stichwörter:: Alkylphenol; Alkylphenolethoxylate; Flusswasser; Fluoreszenzdetektion
∗ Agnieszka Zgoła-Grześkowiak, Poznan University of Technology, Institute of Chemistry and Technical Electrochemistry, ul. Piotrowo 3, 60-965 Poznań, Poland, Phone: +48616652033, Fax: +468616652571, E-Mail:

A. Zgoła-Grześkowiak graduated from the Poznan University of Technology in 1999 and she finished her PhD in 2003 there. Since then she has been working as Assistant Professor at the Poznan University of Technology.

T. Grześkowiak graduated from the Poznan University of Technology in 1995 and he finished his PhD in 2000 there. Since then he has been working outside the university as Quality Control Specialist. Nevertheless, he is realizing his interests working voluntarily at the Poznan University of Technology.

References

1.Giger, W., Brunner, P. H. and Schaffner, C.: Science225 (1984) 623.10.1126/science.6740328Search in Google Scholar

2.Loyo-Rosales, J. E., Schmitz-Alfonso, J., Rice, C. P. and TorrentsA.: Anal. Chem.75 (2003) 4811.10.1021/ac0262762Search in Google Scholar

3.Potter, T. L., Simmons, K., Wu, J., Kostecki, P. and Calabrese, E.: Environ. Sci. Technol.33 (1999) 113.10.1021/es9804464Search in Google Scholar

4.Ahel, M., Hrsak, D. and Giger, W.: Arch. Environ. Contam. Toxicol.26 (1994) 540.10.1007/BF00214159Search in Google Scholar

5.Yoshimura, K.: J. Am. Oil Chem. Soc.63 (1986) 1590.10.1007/BF02553093Search in Google Scholar

6.Manzano, M. A. and Perales, J. A.: Wat. Res.33 (1999) 2593.10.1016/S0043-1354(98)00480-1Search in Google Scholar

7.McLeese, D. W., Zitko, V., Serget, D. B., Burridge, L. and Metcalfe, C. D.: Chemosphere10 (1981) 726.Search in Google Scholar

8.Jobling, S. and Sumpter, J. P.: Aquat. Toxicol.27 (1993) 361.10.1016/0166-445X(93)90064-8Search in Google Scholar

9.White, R., Joblins, S., Koare, S. A., Sumpter, J. P. and Parker, M. G.: Endocrinology135 (1994) 175.10.1210/en.135.1.175Search in Google Scholar

10.Ekelund, R., Bergman, A., Gmo, A. and Berggren, M.: Environ. Pollut.64 (1990) 107.10.1016/0269-7491(90)90108-OSearch in Google Scholar

11.Ahel, M., McEvoy, J. and Giger, W.: Environ. Pollut.79 (1993) 243.10.1016/0269-7491(93)90096-7Search in Google Scholar

12.Sheldon, L. S. and Hites, R. A.: J. Chromatogr. Sci.16 (1978) 49.Search in Google Scholar

13.Loos, R., Hanke, G., Umlauf, G. and Eisenreich, S. J.: Chemosphere66 (2007) 690.10.1016/j.chemosphere.2006.07.060Search in Google Scholar

14.Houde, F., DeBlois, Ch. and Berryman, D. J.: Chromatogr. A961 (2002) 245.10.1016/S0021-9673(02)00661-1Search in Google Scholar

15.Petrovic, M. and Barcelo, D.: J. Mass Spectrom.36 (2001) 1173.10.1002/jms.234Search in Google Scholar

16.Ferguson, P. L., Iden, C. R. and Brownawell, B. J.: Anal. Chem.72 (2000) 4322.10.1021/ac000342nSearch in Google Scholar

17.Planas, C., Guadayol, J. M., Droguet, M., Escalas, A., Rivera, J. and Caixach, J.: Water Res.36 (2002) 982.10.1016/S0043-1354(01)00306-2Search in Google Scholar

18.Loyo-Rosales, J. E., Rice, C. P. and TorrentsA.: Chemosphere68 (2007) 2118.10.1016/j.chemosphere.2007.02.002Search in Google Scholar

19.Schmitz-Alfonso, I., Loyo-Rosales, J. E., de la Paz Aviles, M., Rattner, B. A. and Rice, C. P. J.: Chromatogr. A1010 (2003) 25.10.1016/S0021-9673(03)00956-7Search in Google Scholar

20.Lee, H.-B., Peart, T. E., Bennie, D. T. and Maguire, R. J.: J. Chromatogr. A785 (1997) 385.10.1016/S0021-9673(97)00384-1Search in Google Scholar

21.Holt, M. S., McKerrell, E. H., Perry, J. and Watkinson, R. J.: J. Chromatogr.362 (1986) 419.10.1016/S0021-9673(01)86995-8Search in Google Scholar

22.Careri, M., Elviri, L., Mangia, A. and Zagnoni, I.: Chromatographia57 (2003) 321.10.1007/BF02492403Search in Google Scholar

23.Cheng, Ch.-Y. and Ding, W.-H.: J. Chromatogr. A968 (2002) 143.10.1016/S0021-9673(02)00959-7Search in Google Scholar

24.Zhao, M., van der Wielen, F. and de Voogt, P.: J. Chromatogr. A837 (1999) 129.10.1016/S0021-9673(99)00101-6Search in Google Scholar

25.Nunez, L., Turiel, E. and Tadeo, J. L.: J. Chromatogr. A1146 (2007) 157.10.1016/j.chroma.2007.01.101Search in Google Scholar PubMed

26.John, D. M. and White, G. F.: J. Bacteriology180 (1998) 4332.Search in Google Scholar

27.Lu, J., Jin, Q., He, Y., Wu, J., Zhang, W. and Zhao, J.: Chemosphere71 (2008) 345.10.1016/j.chemosphere.2007.08.068Search in Google Scholar PubMed

28.Schmitt, Th. M.: Analysis of surfactants; Chapter 2, Characterization of Nonionic Surfactants, Marcel Dekker, INC: New York, 1992.Search in Google Scholar

29.Cespedes, R., Lacorte, S., Ginebreda, A. and Barcelo, D.: Environ. Pollut.153 (2008) 384.10.1016/j.envpol.2007.08.026Search in Google Scholar PubMed

30.Cortazar, E., Bartolome, L., Delgado, A., Etxebarria, N., Fernandez, L. A., Usobiaga, A. and Zuloaga, O.: Anal. Chem. Acta534 (2005) 247.10.1016/j.aca.2004.11.037Search in Google Scholar

Received: 2008-12-23
Revised: 2009-03-22
Published Online: 2013-04-02
Published in Print: 2009-07-01

© 2009, Carl Hanser Publisher, Munich

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  2. Contents
  3. Abstracts
  4. Abstracts
  5. Application
  6. Phase Behavior of Engkabang Fat with Nonionic Surfactants
  7. Environmental Chemistry
  8. Liquid Chromatography with Fluores-cence Detection as a Tool for Separation of Endocrine Disrupting Alkylphenols and their Mono- and Diethoxylates in Analysis of River Water Samples
  9. Removal of Brilliant Blue R from Aqueous Solutions on Activated Carbon Produced from Carbonaceous Substrate
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  12. Kinetic and Mechanistic Study of the Influence of Micelles on the Oxidation of Acetone by N-Bromophthalimide in Aqueous Acetic Acid Medium
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https://doi.org/10.3139/113.110024
Keywords for this article
Alkylphenol; alkylphenol ethoxylates; river water; fluorescence detection

Articles in the same Issue

  1. Contents/Inhalt
  2. Contents
  3. Abstracts
  4. Abstracts
  5. Application
  6. Phase Behavior of Engkabang Fat with Nonionic Surfactants
  7. Environmental Chemistry
  8. Liquid Chromatography with Fluores-cence Detection as a Tool for Separation of Endocrine Disrupting Alkylphenols and their Mono- and Diethoxylates in Analysis of River Water Samples
  9. Removal of Brilliant Blue R from Aqueous Solutions on Activated Carbon Produced from Carbonaceous Substrate
  10. Physical Chemistry
  11. Synthesis and Surface Properties of Trisiloxane-modified Oligo(ethylene oxide)
  12. Kinetic and Mechanistic Study of the Influence of Micelles on the Oxidation of Acetone by N-Bromophthalimide in Aqueous Acetic Acid Medium
  13. Synthesis
  14. Synthesis of Cationic Surfactant Intermediates 3-(dimethylamino)-propane-1,2-di-alkylcarbamate
  15. Technical Chemistry
  16. Olefins Derived from Vegetable Oils and their Utilization in Preparation of Surface Active Agent
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