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Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in Penicillium janthinellum MTCC 10889

  • Aditi Kundu EMAIL logo and Rina Ray
Published/Copyright: December 20, 2013
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Chemical Papers
From the journal Chemical Papers Volume 68 Issue 4

Abstract

Amongst various carbon sources, xylan was found to be the sole inducer of endoxylanase production by Penicillium janthinellum MTCC 10889 in submerged cultivation. Endoxylanase synthesis by a xylan induced culture was initially repressed after a simultaneous addition of xylose, probably by the inducer exclusion mechanism, but it was resumed and achieved its highest level at a much later stage of growth (at 120 h). Xylose added after 30 h of growth cannot exert its full repressive effect. Although glucose was proved to be a more potent repressor than xylose, supplementation of salicin, an alcoholic β-glycoside containing d-glucose, with pure xylan resulted in an about 3.22 fold increase in the enzyme synthesis at 72 h followed by constant high production of the enzyme at least until the 144th h of growth. Inducing capacity of salicin in a xylan induced culture was significantly reduced when it was added after 30 h of growth. Addition of salicin and xylan help to partially overcome the repressive effect of xylose and glucose. Failure of salicin in recovering the endoxylanase synthesis in actinomycin D and cyclohexamide inhibited the xylan induced culture indicating that salicin cannot initiate the de novo synthesis of the enzyme.

Keywords: endoxylanase; induction; Penicillium janthinellum; salicin; repression

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Published Online: 2013-12-20
Published in Print: 2014-4-1

© 2013 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.2478/s11696-013-0478-6
Keywords for this article
endoxylanase; induction; Penicillium janthinellum; salicin; repression

Articles in the same Issue

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  4. Effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in Penicillium janthinellum MTCC 10889
  5. Recovery of acetaminophen from aqueous solutions using a supported liquid membrane based on a quaternary ammonium salt as ionophore
  6. Enantioseparation of mandelic acid enantiomers in ionic liquid aqueous two-phase extraction systems
  7. Fatty acid methyl ester production from acid oil using silica sulfuric acid: Process optimization and reaction kinetics
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