Startseite Content of selected secondary metabolites in wild hop
Artikel
Lizenziert
Nicht lizenziert Erfordert eine Authentifizierung

Content of selected secondary metabolites in wild hop

  • Eva Ürgeová EMAIL logo , Katarína Vulganová und Ľudovít Polívka
Veröffentlicht/Copyright: 27. Dezember 2012
Veröffentlichen auch Sie bei De Gruyter Brill

Abstract

We focused on the determination of biologically active secondary metabolites in wild hops over the course of the vegetation period in four selected sites in Piešťany. Hop was collected in four collection periods in 2010. The objects of research were the leaves of male and female plants, and female cones. Analysis of the extracts from wild hop confirmed differences in total content of polyphenols, flavonoids between the localities and in the growing seasons over the vegetation period. The extracts from leaves from the first harvest in localities “gSládkovičova” and the river Vah showed higher levels of polyphenols (4.91–6.93 mg g−1 of dry mass), flavonoids (2.28–2.99 mg g−1 of dry mass) than the extracts from cones collected at the end of the vegetation period (polyphenols 3.63–5.33 mg g−1 of dry mass, flavonoids 1.86–2.16 mg g−1 of dry mass). The extracts from leaves from the first harvest from the “tennis court” site showed lower values of polyphenols and flavonoids. Our findings were that the leaves from the first harvest at the beginning of the growing season contained higher amounts of the secondary metabolites investigated than the cones at the end of the growing season. The content of α-bitter acids in wild hop cones ranged from 1.64 % to 2.91 %, in leaves from 0.11 % to 0.99%. Concentration of β-bitter acids in cones varied from 1.63 % to 1.93 % and in leaves from 0.02 % to 0.61 %.

[1] Barros, L., Dueñas, M., Dias, M. I., Sousa, M. J., Santos-Buelga, C., & Ferreira, I. C. F. R. (2012). Phenolic profiles of in vivo and in vitro grown Coriandrum sativum L. Food Chemistry, 132, 841–848. DOI: 10.1016/j.foodchem.2011.11.048. http://dx.doi.org/10.1016/j.foodchem.2011.11.04810.1016/j.foodchem.2011.11.048Suche in Google Scholar

[2] Collin, H. A. (2001). Secondary product formation in plant tissue cultures. Plant Growth Regulation, 34, 119–134. DOI: 10.1023/a:1013374417961. http://dx.doi.org/10.1023/A:101337441796110.1023/A:1013374417961Suche in Google Scholar

[3] Čeh, B., Kač, M., Košir, I. J., & Abram, V. (2007). Relationships between xanthohumol and polyphenol content in hop leaves and hop cones with regard to water supply and cultivar. International Journal of Molecular Sciences, 8, 989–1000. DOI: 10.3390/i8090989. http://dx.doi.org/10.3390/i809098910.3390/i8090989Suche in Google Scholar

[4] De Keukeleire, J., Ooms, G., Heyerick, A., Roldan-Ruiz, I., Van Bockstaele, E., & De Keukeleire, D. (2003). Formation and accumulation of α-acids, β-acids, desmethylxanthohumol, and xanthohumol during flowering of hops (Humulus lupulus L.). Journal of Agricultural and Food Chemistry, 51, 4436–4441. DOI: 10.1021/jf034263z. http://dx.doi.org/10.1021/jf034263z10.1021/jf034263zSuche in Google Scholar

[5] Delmulle, L., Bellahcène, A., Dhooge, W., Comhaire, F., Roelens, F., Huvaere, K., Heyerick, A., Castronovo, V., & De Keukeleire, D. (2006). Anti-proliferative properties of prenylated flavonoids from hops (Humulus lupulus L.) in human prostate cancer cell lines. Phytomedicine, 13, 732–734. DOI: 10.1016/j.phymed.2006.01.001. http://dx.doi.org/10.1016/j.phymed.2006.01.00110.1016/j.phymed.2006.01.001Suche in Google Scholar

[6] Hampton, R., Nickerson, G., Whitney, P., & Haunold, A. (2002). Erratum to “Comparative chemical attributes of native North American hop, Humulus lupulus var. lupuloides E. Small”. Phytochemistry, 63, 737–738. DOI: 10.1016/s0031- 9422(03)00248-6. http://dx.doi.org/10.1016/S0031-9422(03)00248-610.1016/S0031-9422(03)00248-6Suche in Google Scholar

[7] Hofta, P., Dostálek P., & Basarová, G. (2004). Xantohumol — chmelová pryskyrice nebo polyfenol? Chemické Listy, 98, 825–830. Suche in Google Scholar

[8] Jelínek, L., Šneberger, M., Karabín, M., & Dostálek, P. (2010). Comparison of Czech hop cultivars based on their contents of secondary metabolites. Czech Journal of Food Sciences, 28, 309–316. 10.17221/65/2010-CJFSSuche in Google Scholar

[9] Krofta, K. (2006). Spektrofotometrické stanovení alfa a beta hořkých kyselin (ASBC). Žatec, Czech Republic: Hop Research Institute. Suche in Google Scholar

[10] Naczk, M., & Shahidi, F. (2004). Extraction and analysis of phenolics in food. Journal of Chromatography A, 1054, 95–111. DOI: 10.1016/j.chroma.2004.08.059. 10.1016/S0021-9673(04)01409-8Suche in Google Scholar

[11] Patzak, J., Nesvadba, V., Henychová, A., & Krofta, K. (2010). Assessment of the genetic diversity of wild hops (Humulus lupulus L.) in Europe using chemical and molecular analyses. Biochemical Systematics and Ecology, 38, 136–145. DOI: 10.1016/j.bse.2009.12.023. http://dx.doi.org/10.1016/j.bse.2009.12.02310.1016/j.bse.2009.12.023Suche in Google Scholar

[12] Piendl, A., & Biendl, M. (2000). Physiological significance of polyphenols and hop bitters in beer. Brauwelt International, 18, 310–317. Suche in Google Scholar

[13] Rakotoarison, D. A., Gressier, B., Trotin, F., Brunet, C., Dine, T., Luyckx, M., Vasseur, J., Cazin, M., Cazin, J. C., & Pinkas, M. (1997). Antioxidant activities of polyphenolic extracts from flowers, in vitro callus and cell suspension cultures of Crataegus monogyna. Pharmazie, 52, 60–63. Suche in Google Scholar

[14] SHMÚ (2010). Written information on request about climatological conditions from the climatological station Piešťany. Bratislava, Slovakia: Slovak Hydrometeorological Institute (SHMÚ). Suche in Google Scholar

[15] Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158. Suche in Google Scholar

[16] Ürgeová, E., & Polívka, L. (2009). Secondary metabolites with antibacterial effects from leaves of different hop cultivars during vegetal periods. Nova Biotechnologica, 9, 327–332. Suche in Google Scholar

[17] Vašková, M. (2009). Stanovenie vybraných sekundárnych metabolitov s biocídnym úcinkom v rôznych odrodách chmelu obycajného pocas vegetácie. Rigorous thesis. University of SS. Cyril and Methodius, Trnava, Slovakia. Suche in Google Scholar

[18] Zanoli, P., & Zavatti, M. (2008). Pharmacognostic and pharmacological profile of Humulus lupulus L. Journal of Ethnopharmacology, 116, 383–396. DOI: 10.1016/j.jep.2008.01.011. http://dx.doi.org/10.1016/j.jep.2008.01.01110.1016/j.jep.2008.01.011Suche in Google Scholar PubMed

Published Online: 2012-12-27
Published in Print: 2013-3-1

© 2012 Institute of Chemistry, Slovak Academy of Sciences

Heruntergeladen am 27.11.2025 von https://www.degruyterbrill.com/document/doi/10.2478/s11696-012-0213-8/pdf
Button zum nach oben scrollen